Molecular profiling leading to personalized cancer treatment

Excerpt

Personalized medicine and molecular profiling represent inseparable terms. Only with the broad applicability and low-level access to genomics and next generation sequencing techniques has it been possible to implement precision medicine in the daily routine of medical oncology. In fact, the increasing feasibility of not solely tissue biopsies but also less invasive liquid biopsies is currently again offering new opportunities. As of 2022, molecular tumor profiling on (archived) formalin-fixed, paraffin-embedded (FFPE) tissues using DNA/RNA sequencing techniques allows identification of a wide number of cancer growth-related mutations and fusions that may proceed to treatment planning. However, we had to learn in the past that even if a druggable target exists, it is not given that it (1) is a defined driver of the distinct disease and (2) is clinically significant in the specific setting. Consequently, molecular profiling requests not only the technical abilities to perform such testing but also deep and concise understanding of the target, corresponding drugs, and tumor biology. Furthermore, we need to distinguish clinical settings where no standard is available and next generation sequencing is used to identify potential further therapies, such as in the relapsed/refractory setting after exhaustion of evidenced-based therapies (with multiple successful examples previously published, e.g., the Austrian example set in the EXACT trial by Prager G et al. [ 1]), versus settings where molecular targeted treatments are essential to the standard treatment algorithm. For the latter, EGFR-driven therapies in lung cancer constitute one of the earliest successful applications and recently a number of novel targeted drugs were approved in lung cancer, including KRAS, MET, and RET inhibitors. Hence, for both the salvage setting and increasingly for approved standard treatments, molecular profiling is crucial for optimal patient management. …