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01.10.2012 | Original Scientific Paper

Effects of everolimus on hepatic ischemia/reperfusion injury in an experimental rat model

verfasst von: Dr. B. G. Demirci, M.D., Prof. Dr. M. Cindoruk, U. T. Yilmaz, M. D. Demirag, I. I. Gonul, U. Demirci, O. Gulbahar, Prof. Dr. A. Dalgic

Erschienen in: European Surgery | Ausgabe 5/2012

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Summary

Background

The current study is the first to evaluate the protective effect of everolimus on biochemical and histopathological features with an experimental hepatic ischemia reperfusion (I/R) injury model.

Methods

Twenty-four Wistar Albino rats were randomized into four groups: Group 1 (sham group) was subjected to laparotomy. Group 2 (I/R group) was subjected to ischemia for 1 hour and reperfusion for 24 hours without treatment. Group 3 was treated with 1.5 mg/kg/day everolimus perorally for 7 days without performing I/R. Group 4 was treated with 1.5 mg/kg/day everolimus perorally for 7 days followed with performing I/R. Blood samples and liver tissues were obtained to assess serum aspartate aminotransferase (AST), alanin aminotransferase (ALT), albumin, tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6) and tissue malonyl dialdehyde (MDA), and superoxide dismutase (SOD) levels and histopathologic examination.

Results

Serum AST and ALT levels of I/R group were significantly higher than sham group. Serum transaminase levels were significantly decreased after treatment with everolimus (group 4) when compared with I/R group (group 2). TNFα and MDA levels were decreased; however, SOD levels were increased in group 4. The sham group and group 3 showed normal histological findings. Morphologic analysis showed reduced degrees of injury in group 4 than group 2 with regards to cholestasis, sinusoidal dilatation, congestion, hydropic degeneration, and subcapsular necrosis.

Conclusions

This is the first report as regards everolimus attenuates biochemical and histopathological alterations of hepatic I/R injury. Everolimus can be a choice for preventing I/R injury during liver transplantation surgery in the future besides its well-known immunosuppressive property.
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Metadaten
Titel
Effects of everolimus on hepatic ischemia/reperfusion injury in an experimental rat model
verfasst von
Dr. B. G. Demirci, M.D.
Prof. Dr. M. Cindoruk
U. T. Yilmaz
M. D. Demirag
I. I. Gonul
U. Demirci
O. Gulbahar
Prof. Dr. A. Dalgic
Publikationsdatum
01.10.2012
Verlag
Springer Vienna
Erschienen in
European Surgery / Ausgabe 5/2012
Print ISSN: 1682-8631
Elektronische ISSN: 1682-4016
DOI
https://doi.org/10.1007/s10353-012-0152-1

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