OutcomeTime Course of Antioxidant Enzyme Activities in Liver Transplant Recipients
Section snippets
Materials and methods
The 13 adult patients (43 to 57 years old) included 11 men and 2 women. Written informed consent was obtained from the patients’ relatives. The study protocol was approved by the local Clinical Research (Ethics) Committee. Blood samples containing EDTA as anticoagulant were obtained pre- and postsurgical intervention before transplant (T0), and 1, 6, and 12 hours, as well as 1, 2, 3, 5, and 7 days after liver transplantation. We determined total and specific SOD activity, CAT, GPX, and GR
Results
SOD activity was enhanced at 1 hour after transplantation relative to the value before transplantation (T0). The activity returned to pretransplant values at 1 day after the procedure, increasing again from day 2 (Table 1). The pattern of SOD activity before and after liver transplantation was followed by staining nondenaturing (native) polyacrylamide gels for SOD activity. The activity was mainly represented by Mn-SOD (75%) and Cu,Zn-SOD (25%), whereas Fe-SOD was not detected (Fig 1). CAT
Discussion
The results obtained herein confirmed the presence of an oxidative stress. The enhanced SOD activity represents the response of the organism toward superoxide radicals generated during and after liver transplantation. A consequence of this activity is the generation of hydrogen peroxide, which is decomposed by CAT activity. Lipid peroxidation is the principal damage caused by ROS. MDA, one of the final products in the peroxidation process, serves as an indication of the amount of generated ROS.
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2017, Redox BiologyCitation Excerpt :Related to this study, it has also been described that the levels of lipid peroxidation increase at 1 h post-operation and are maintained over basal levels up to 5 days following liver transplant. Lipid peroxidation in turn correlates with the upregulation of enzymatic antioxidants such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione reductase (GR) [69]. Furthermore, the level of lipid peroxidation also correlates with pre-operative hepatic injury markers such as bilirubin, AST, ALT, model of end-stage liver disease score (MELD score), International Normalized Ratio (INR) and with expression of pro-inflammatory cytokines [73].
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Supported by grants FIS 03/0968 and C03/03 from the Instituto Salud Carlos III (Ministery of Health, Spain).