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Erschienen in: Wiener Medizinische Wochenschrift 7-8/2015

01.04.2015 | main topic

Ancient pathogens in museal dry bone specimens: analysis of paleocytology and aDNA

verfasst von: Johanna Sophia Gaul, Eduard Winter, Asst.-Prof. Dr. Karl Grossschmidt

Erschienen in: Wiener Medizinische Wochenschrift | Ausgabe 7-8/2015

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Summary

Bone samples investigated in this study derive from the pathologic–anatomical collection of the Natural History Museum of Vienna. In order to explore the survival of treponemes and treponemal ancient DNA in museal dry bone specimens, we analyzed three individuals known to have been infected with Treponema pallidum pallidum. No reproducible evidence of surviving pathogen’s ancient DNA (aDNA) was obtained, despite the highly sensitive extraction and amplification techniques (TPP15 and arp). Additionally, decalcification fluid of bone sections was smear stained with May-Gruenwald-Giemsa. The slides were examined using direct light microscope and dark field illumination. Remnants of spirochetal structures were detectable in every smear. Our results demonstrate that aDNA is unlikely to survive, but spirochetal remains are stainable and thus detectable.
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Metadaten
Titel
Ancient pathogens in museal dry bone specimens: analysis of paleocytology and aDNA
verfasst von
Johanna Sophia Gaul
Eduard Winter
Asst.-Prof. Dr. Karl Grossschmidt
Publikationsdatum
01.04.2015
Verlag
Springer Vienna
Erschienen in
Wiener Medizinische Wochenschrift / Ausgabe 7-8/2015
Print ISSN: 0043-5341
Elektronische ISSN: 1563-258X
DOI
https://doi.org/10.1007/s10354-015-0357-6

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