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Erschienen in: European Surgery 2/2015

01.04.2015 | Original Article

The effects of sulforaphane in the rat model of experimental intestinal ischemia reperfusion

verfasst von: MD T. Talih, MD Assist. Prof. E. Arslan, MD Prof. E. Sozuer, MD Prof. A. Akcan, MD Assoc. Prof. H. Akyıldız, MD Assist. Prof. M. Akyüz, MD Prof. C. Yazıcı

Erschienen in: European Surgery | Ausgabe 2/2015

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Summary

Aim

The aim of this study is to investigate the effects of sulforaphane (SFN), which has been shown to protect such organs as the brain, kidneys, liver, and heart against the injury caused by ischemia reperfusion (I-R), on the reperfusion injury in the experimental intestinal I-R model.

Material and method

The study employed 30 Wistar albino rats, and there were three groups: the control group (n = 10), I-R group (n = 10), and SFN + I-R group (n = 10). The rats in the control group were sacrificed after a 3-h follow-up subsequent to the laparotomy. The I-R group was subjected to an hour of ischemia and 2 h of reperfusion afterward. The SFN + I-R group was administered a dose of 3 mg/kg SFN intraperitoneally. The rats were sacrificed after the ileum resection for measurement of tissue malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity and histopathological analysis.

Results

The I-R and SFN + I-R groups were histopathologically shown to have similar cell injuries. In the SFN + I-R group, the increase in GSH-Px activity was higher than SOD activity and both of the increases were statistically significant compared with the control group (p < 0.001). The SFN application did not yield a significant difference in the tissue MDA level compared with the control group.

Conclusion

The effect of SFN use on the I-R injury in the intestine did not show an apparent effect histopathologically, although there were statistically significant differences in biochemical parameters.
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Metadaten
Titel
The effects of sulforaphane in the rat model of experimental intestinal ischemia reperfusion
verfasst von
MD T. Talih
MD Assist. Prof. E. Arslan
MD Prof. E. Sozuer
MD Prof. A. Akcan
MD Assoc. Prof. H. Akyıldız
MD Assist. Prof. M. Akyüz
MD Prof. C. Yazıcı
Publikationsdatum
01.04.2015
Verlag
Springer Vienna
Erschienen in
European Surgery / Ausgabe 2/2015
Print ISSN: 1682-8631
Elektronische ISSN: 1682-4016
DOI
https://doi.org/10.1007/s10353-015-0307-y

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