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Erschienen in: Wiener klinische Wochenschrift 17-18/2023

19.04.2023 | original article

CircRNA circSLIT2 is a novel diagnostic and prognostic biomarker for gastric cancer

verfasst von: Longyue Wang, Shuaishuai Xiao, Yiming Zheng, Zefeng Gao

Erschienen in: Wiener klinische Wochenschrift | Ausgabe 17-18/2023

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Summary

Background

Many diseases can mimic the symptoms of gastric cancer (GC). Therefore, misdiagnosis of GC is common. Our preliminary sequencing analysis revealed the altered expression of circSLIT2 in GC. In this study, we further explored the role of circSLIT2 in GC.

Methods

The research subjects included GC patients, patients with irritable bowel syndrome (IBS), patients with gastric ulcer (GU), patients with gastric tuberculosis (GT), patients with Crohn’s disease (CD), and healthy controls (HC). Accumulation of circSLIT2 RNA in both tissue and plasma samples was determined with RT-qPCR. The diagnostic and prognostic values of circSLIT2 for GC were explored by performing ROC and survival curve analysis. The χ2-test was applied for association analysis.

Results

Increased circSLIT2 RNA accumulation was observed in GC tissues compared to non-tumor tissues. Compared to the HC group, increased plasma circSLIT2 RNA accumulation was only observed in the GC group, but not in the IBS, GU, GT, and CD groups. Plasma circSLIT2 showed a positive correlation with circSLIT2 in GC tissues but not circSLIT2 in non-tumor tissues. Using increased plasma circSLIT2 as a biomarker, GC patients were effectively separated from other disease groups and the HC group. Survival curve analysis revealed that most patients who died during the 5‑year follow-up had high levels of circSLIT2 accumulation in GC tissues and plasma. CircSLIT2 in plasma and GC tissue was only closely associated with distant tumor metastases, but not other clinical factors.

Conclusion

Increased circSLIT2 accumulation may serve as a novel diagnostic and prognostic biomarker for GC.
Literatur
11.
Zurück zum Zitat Danzi JT, Farmer RG, Sullivan BH Jr., Rankin GB. Endoscopic features of gastroduodenal Crohn’s disease. Gastroenterology. 1976;70(1):9–13.CrossRefPubMed Danzi JT, Farmer RG, Sullivan BH Jr., Rankin GB. Endoscopic features of gastroduodenal Crohn’s disease. Gastroenterology. 1976;70(1):9–13.CrossRefPubMed
17.
Zurück zum Zitat Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 ΔΔCT method[J]. methods 25(4):402–408CrossRefPubMed Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 ΔΔCT method[J]. methods 25(4):402–408CrossRefPubMed
Metadaten
Titel
CircRNA circSLIT2 is a novel diagnostic and prognostic biomarker for gastric cancer
verfasst von
Longyue Wang
Shuaishuai Xiao
Yiming Zheng
Zefeng Gao
Publikationsdatum
19.04.2023
Verlag
Springer Vienna
Erschienen in
Wiener klinische Wochenschrift / Ausgabe 17-18/2023
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI
https://doi.org/10.1007/s00508-023-02155-x

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