nach oben

Erschienen in:

01.01.2017 | original article

Cilastatin does not affect Carba NP test performance for detection of carbapenemase production in Enterobacteriaceae

verfasst von: Dr. Rainer Hartl, Sarah Widhalm, Dr. Heidrun Kerschner, Univ. Prof. Petra Apfalter, Dr. Rainer Gattringer

Erschienen in: Wiener klinische Wochenschrift | Ausgabe 1-2/2017

Einloggen, um Zugang zu erhalten

Summary

The Carba NP test is a simple confirmation method for carbapenemase-producing Enterobacteriaceae (CPE) but reagents have to be freshly prepared as imipenem sodium salt is unstable. We evaluated the Carba NP test performance based on a commercially available 10-fold cheaper drug formulation containing cilastatin against 217 CPE and 78 non-CPE isolates with reduced meropenem susceptibility. Specificity and sensitivity were 100 % and 98.6 %, respectively and 3 false negative results of blaVIM-1-producing Proteus mirabilis were reproducible with the RAPIDEC® Carba NP test. Cilastatin does not disturb test performance provided that the imipenem drug quantity is doubled.

Vorheriger Artikel Determinants of maternal vitamin D concentrations in Slovenia

Nächster Artikel Pretransplantation monitoring of HLA antibodies by complement dependent cytotoxicity and Luminex-based assays

Nordmann P, Dortet L, Poirel L. Carbapenem resistance in enterobacteriaceae: Here is the storm! Trends Mol Med. 2012;18:263–72.CrossRefPubMed

Grundmann H, Livermore DM, Giske CG, et al. Carbapenem-non-susceptible enterobacteriaceae in Europe: conclusions from a meeting of national experts. Euro Surveill. 2010;15(46):pii=19711.

Tacconelli E, Cataldo MA, Dancer SJ, et al. Escmid guidelines for the management of the infection control measures to reduce transmission of multidrug-resistant gram-negative bacteria in hospitalized patients. Clin Microbiol Infect. 2014;20(Suppl):1–55.CrossRefPubMed

Nordmann P, Poirel L. The difficult-to-control spread of carbapenemase producers among enterobacteriaceae worldwide. Clin Microbiol Infect. 2014;20:821–30.CrossRefPubMed

Nordmann P, Poirel L, Dortet L. Rapid detection of carbapenemase-producing enterobacteriaceae. Emerg Infect Dis. 2012;18:1503–7.CrossRefPubMedPubMedCentral

Tijet N, Boyd D, Patel SN, Mulvey MR, Melano RG. Evaluation of the carba np test for rapid detection of carbapenemase-producing enterobacteriaceae and pseudomonas aeruginosa. Antimicrob Agents Chemother. 2013;57:4578–80.CrossRefPubMedPubMedCentral

Osterblad M, Hakanen AJ, Jalava J. Evaluation of the carba np test for carbapenemase detection. Antimicrob Agents Chemother. 2014;58:7553–6.CrossRefPubMedPubMedCentral

Dortet L, Brechard L, Poirel L, Nordmann P. Impact of the isolation medium for detection of carbapenemase-producing enterobacteriaceae using an updated version of the carba np test. J Med Microbiol. 2014;63:772–6.CrossRefPubMed

Giske CG, Gezelius L, Samuelsen O, Warner M, Sundsfjord A, Woodford N. A sensitive and specific phenotypic assay for detection of metallo-beta-lactamases and kpc in klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clin Microbiol Infect. 2011;17:552–6.CrossRefPubMed

10.

Naas T, Cuzon G, Bogaerts P, Glupczynski Y, Nordmann P. Evaluation of a DNA microarray (check-mdr ct102) for rapid detection of tem, shv, and ctx-m extended-spectrum beta-lactamases and of kpc, oxa-48, vim, imp, and ndm-1 carbapenemases. J Clin Microbiol. 2011;49:1608–13.CrossRefPubMedPubMedCentral

11.

Dallenne C, Da Costa A, Decre D, Favier C, Arlet G. Development of a set of multiplex pcr assays for the detection of genes encoding important beta-lactamases in enterobacteriaceae. J Antimicrob Chemother. 2010;65:490–5.CrossRefPubMed

12.

Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex pcr for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis. 2011;70:119–23.CrossRefPubMed

13.

Vasoo S, Cunningham SA, Kohner PC, et al. Comparison of a novel, rapid chromogenic biochemical assay, the carba np test, with the modified hodge test for detection of carbapenemase-producing gram-negative bacilli. J Clin Microbiol. 2013;51:3097–101.CrossRefPubMedPubMedCentral

14.

Keynan S, Hooper NM, Felici A, Amicosante G, Turner AJ. The renal membrane dipeptidase (dehydropeptidase i) inhibitor, cilastatin, inhibits the bacterial metallo-beta-lactamase enzyme cpha. Antimicrob Agents Chemother. 1995;39:1629–31.CrossRefPubMedPubMedCentral

15.

Adler A, Assous MV, Paikin S, et al. Emergence of vim-producing aeromonas caviae in israeli hospitals. J Antimicrob Chemother. 2014;69(5):1211–4.CrossRefPubMed

16.

Dortet L, Poirel L, Nordmann P. Rapid detection of carbapenemase-producing pseudomonas spp. J Clin Microbiol. 2012;50:3773–6.CrossRefPubMedPubMedCentral

17.

Dortet L, Poirel L, Errera C, Nordmann P. Carbacineto np test for rapid detection of carbapenemase-producing acinetobacter spp. J Clin Microbiol. 2014;52:2359–64.CrossRefPubMedPubMedCentral

Titel: Cilastatin does not affect Carba NP test performance for detection of carbapenemase production in Enterobacteriaceae
verfasst von: Dr. Rainer Hartl
Sarah Widhalm
Dr. Heidrun Kerschner
Univ. Prof. Petra Apfalter
Dr. Rainer Gattringer
Publikationsdatum: 01.01.2017
Verlag: Springer Vienna
Erschienen in: Wiener klinische Wochenschrift / Ausgabe 1-2/2017
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI: https://doi.org/10.1007/s00508-016-1106-6

Springer Medizin Österreich

Summary

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1-2/2017

Epidermolysis bullosa House Austria and Epidermolysis bullosa clinical network

Vaccine hesitancy in Austria

Glycemia management in a Slovenian general hospital

The medical and scientific responsibility of pollen information services

Diagnostic accuracy of the 14C-urea breath test in Helicobacter pylori infections: a meta-analysis

Veranstaltungen