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01.09.2013 | original article

Expression of β-catenin and cyclin D1 in Merkel cell carcinomas of the head and neck

verfasst von: Asst Prof Claudia Lill, MD, PD, Sven Schneider, MD, Bahil Ghanim, MD, Markus Brunner, MD, PD, Prof Gregor Heiduschka, MD, Prof Robert Loewe, MD, Prof Dietmar Thurnher, MD

Erschienen in: Wiener klinische Wochenschrift | Ausgabe 17-18/2013

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Summary

Background

Merkel cell carcinomas (MCC) are very aggressive tumors of the sun-exposed skin with a high potential to metastasize. Little is known about the genesis of MCC and very few prognostic markers have been detected so far. The Wnt pathway protein β-catenin and the cell cycle protein cyclin D1 are two promotors of tumor growth and are expressed in a variety of malignant neoplasms such as lymphomas, thyroid, breast cancer, and many others.

Patients and Methods

Tissue samples of 27 patients with MCC were immunohistochemically stained for β-catenin and cyclin D1 and correlated with overall survival of patients. In addition, western blot analysis was carried out in the two MCC cell lines MCC-13 and MCC-26.

Results

β-catenin showed a cytoplasmatic expression of 10–30 % in 11 samples and an expression lower than 10 % in eight samples. Nuclear staining was visible in two samples. None of the 27 samples expressed cyclin D1.

Conclusion

Neither cyclin D1 nor β-catenin was expressed in a statistically significant manner, concluding that the development of MCCs is independent of β-catenin and cyclin D1 expression and these proteins are not suitable as prognostic markers. We could describe the expression pattern of cyclin D1 for the first time.

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Titel: Expression of β-catenin and cyclin D1 in Merkel cell carcinomas of the head and neck
verfasst von: Asst Prof Claudia Lill, MD, PD
Sven Schneider, MD
Bahil Ghanim, MD
Markus Brunner, MD, PD
Prof Gregor Heiduschka, MD
Prof Robert Loewe, MD
Prof Dietmar Thurnher, MD
Publikationsdatum: 01.09.2013
Verlag: Springer Vienna
Erschienen in: Wiener klinische Wochenschrift / Ausgabe 17-18/2013
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI: https://doi.org/10.1007/s00508-013-0406-3

Springer Medizin Österreich

Summary

Background

Patients and Methods

Results

Conclusion

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