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A Novel Combined Imaging/Morphometrical Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis

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Cell Imaging Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 931))

Abstract

Nerve Morphometry is one tool employed in the clinical assessment of peripheral sural nerve pathological abnormalities. A new method is presented in this chapter incorporating an unbiased approach to quantitative sural nerve evaluation. Using conventional epoxy embedded nerves processed for electron microscopy, confocal microscopy, and interactive digital assessment, this method produces a rigorous, accurate reproducible record for use in clinical diagnosis.

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Acknowledgement

The project described in this chapter was supported by Award Number 1S10RR019246 from the National Center for Research Resources (to DJT) for purchase of the Zeiss 510 META confocal scanning laser microscope.

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Authors and Affiliations

  1. Department of Pathology and Microscopy Imaging Center, College of Medicine, University of Vermont, Burlington, VT, USA

    Michele A. vonTurkovich, Marilyn P. Wadsworth & Douglas J. Taatjes

  2. Department of Pathology, College of Medicine, University of Vermont, Burlington, VT, USA

    William W. Pendlebury

Authors
  1. Michele A. vonTurkovich
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  2. Marilyn P. Wadsworth
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  3. William W. Pendlebury
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  4. Douglas J. Taatjes
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Corresponding author

Correspondence to Douglas J. Taatjes .

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Editors and Affiliations

  1. College of Medicine, Professor, Department of Pathology, University of Vermont, Beaumont Ave 89, Burlington, 05405-1742, Vermont, USA

    Douglas J. Taatjes

  2. , Abt. Zell- und Molekularpathologie, Universität Zürich, Schmelzbergstr. 12, Zürich, 8091, Switzerland

    Jürgen Roth

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© 2012 Springer Science+Business Media, LLC

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vonTurkovich, M.A., Wadsworth, M.P., Pendlebury, W.W., Taatjes, D.J. (2012). A Novel Combined Imaging/Morphometrical Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis. In: Taatjes, D., Roth, J. (eds) Cell Imaging Techniques. Methods in Molecular Biology, vol 931. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-056-4_19

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  • DOI: https://doi.org/10.1007/978-1-62703-056-4_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-055-7

  • Online ISBN: 978-1-62703-056-4

  • eBook Packages: Springer Protocols

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