Abstract
This chapter describes the application of diagnostic HLA typing for disease association and five methods used for specific HLA genotypes. The methods utilise a combination of polymerase chain reaction (PCR) amplification to detect sequence polymorphism by the presence or absence of amplification, nucleotide sequencing of the PCR product, and hybridisation of the PCR product with labelled probes. The probes are specific for sequence polymorphism associated with the genotype and are attached to either a Micro Bead or a Solid Phase. In addition, the detection of single nucleotide polymorphism(s) which “tag” for the genotype using a real-time PCR is described.
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Acknowledgements
The DR/DQ SSP, SBT, and micro bead hybridisation methods are a culmination of input by the molecular typing staff of the Transplantation and Immunogenetics Service of the Australian Red Cross Blood Service.
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Varney, M.D., Castley, A.S.L., Haimila, K., Saavalainen, P. (2012). Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_3
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DOI: https://doi.org/10.1007/978-1-61779-842-9_3
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