Abstract
The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target “detector” cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of “heterologous immunity” from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.
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References
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D’Orsogna, L.J., van der Meer-Prins, E.M.W., Zoet, Y.M., Roelen, D.L., Doxiadis, I.I.N., Claas, F.H.J. (2012). Detection of Allo-HLA Cross-Reactivity by Virus-specific Memory T-Cell Clones Using Single HLA-Transfected K562 Cells. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_19
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DOI: https://doi.org/10.1007/978-1-61779-842-9_19
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-841-2
Online ISBN: 978-1-61779-842-9
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