Abstract
Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.
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Semeins, C.M., Bakker, A.D., Klein-Nulend, J. (2012). Isolation of Primary Avian Osteocytes. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_4
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DOI: https://doi.org/10.1007/978-1-61779-415-5_4
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