Abstract
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification of this enzyme facilitates studies of its structure and mechanism and allows elucidation of native features of both wild-type- and human disease-related forms.
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References
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Ziebarth, T.D., Kaguni, L.S. (2009). Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase. In: Stuart, J.A. (eds) Mitochondrial DNA. Methods in Molecular Biology™, vol 554. Humana Press. https://doi.org/10.1007/978-1-59745-521-3_8
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DOI: https://doi.org/10.1007/978-1-59745-521-3_8
Publisher Name: Humana Press
Print ISBN: 978-1-934115-60-2
Online ISBN: 978-1-59745-521-3
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