Summary
Dendritic cells (DCs) play a key role in the initiation of effective immune responses against infectious agents because they are unique in their ability to provide antigen-specific activation of naïve T cells. To do this, they must acquire antigen and migrate to spleen or lymph node to present the antigen to T cells in association with costimulatory molecules and cytokines. Murine models of chronic EtOH exposure have been developed for dissecting the mechanisms by which EtOH alters immune cell functions. This chapter details methods for assessing DC functions in such models. Methods are presented for 1) the identification and isolation of various DC subsets from spleen, epidermis, and lung, 2) measurement of LC migration out of epidermis and DC migration into peripheral and peribronchial lymph nodes, and 3) measurement of alloantigen presentation in vitro as well as transgenic T-cell activation in vitro and in vivo.
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References
1. Shortman, K. (2002) Mouse and human dendritic cell subtypes. Nat. Rev. Immunol. 2, 151–161.
2. Randolph, G. J., Angeli, V., and Swartz, M. A. (2005) Dendritic-cell trafficking to lymph nodes through lymphatic vessels. Nat. Rev. Immunol. 5, 617–628.
3. de Jong, E. C., Smits, H. H., and Kapsenberg, M. L. (2005) Dendritic cell-mediated T cell polarization. Spring. Sem. Immunol. 26, 289–307.
4. Valladeau, J., Saeland, S. (2005) Cutaneous dendritic cells. Seminars in Immunology 17, 273–283.
5. Steinman, R. M., Hawiger, D., Nussenzweig, M. C. (2003) Tolerogenic dendritic cells. Ann. Rev. Immunol. 21, 685–711.
6. Bagasra, O., Howeedy, A., Kajdacsy-Balla, A. (1988) Macrophage function in chronic experimental alcoholism. Immunol. 65, 405–409.
7. Castro, A., Lefkowitz, D. L., Lefkowitz, S. S. (1993) The effects of alcohol on murine macrophage function. Life Sci. 52, 1585–1593.
8. Mikszta, J., Waltenbaugh, C., Kim, B. S. (1995) Impaired antigen presentation by splenocytes of ethanol-consuming C57BL/6 mice. Alcohol 12, 265–271.
9. Waltenbaugh, C., Peterson, J. D. (1997) Ethanol impairs the induction of delayed hypersensitivity in C57BL/6 mice. Alcohol 14, 149–153.
10. Wang, K., Busker-Mannie, A. E., Hoeft, J., Vasquez, K., Miller, S. D., Melvold, R. W., Waltenbaugh, C. (2001) Prolonged Hya-disparate skin graft survival in ethanol-consuming mice: correlation with impaired delayed hypersensitivity. Alcohol. Clin. Exp. Res. 25, 1542–1548.
11. Zisman, D. A., Strieter, R. M., Kunkel, S. L., Tsai, W. C., Wilkowski, J. M., Bucknell, K. A., Standiford, T. J. (1998) Ethanol feeding impairs innate immunity and alters the expression of Th1- and Th2-phenotype cytokines in murine Klebsiella pneumonia. Alcohol. Clin. Exp. Res. 22, 621–627.
12. Peterson, J. D., Vasquez, K., Waltenbaugh, C. (1998) Interleukin-12 therapy restores cell-mediated immunity in ethanol-consuming mice. Alcohol. Clin. Exp. Res. 22, 245–251.
13. Waltenbaugh, C., Vasquez, K., Peterson, J. D. (1998) Alcohol consumption alters antigen-specific Th1 responses: mechanisms of deficit and repair. Alcohol. Clin. Exp. Res. 22, 220S-223S.
14. Cumberbatch, M., Dearman, R. J., Kimber, I. (1996) Constitutive and inducible expression of interleukin-6 by Langerhans cells and lymph node dendritic cells. Immunol. 87, 513–518.
15. Jerrells, T. R., Sibley, D. (1995) Effects of ethanol on cellular immunity to facultative intracellular bacteria. Alcohol. Clin. Exp. Res. 19, 11–16.
16. Standiford, T. J., Danforth, J. M. (1997) Ethanol feeding inhibits proinflammatory cytokine expression from murine alveolar macrophages ex vivo. Alcohol. Clin. Exp. Res. 21, 1212–1217.
17. D'Souza, N. B., Nelson, S., Summer, W. R., Deaciuc, I. V. (1996) Alcohol modulates alveolar macrophage tumor necrosis factor-alpha, superoxide anion, and nitric oxide secretion in the rat. Alcohol. Clin. Exp. Res. 21, 156–163.
18. Lau, A. H., Abe, M., Thomson, A. W. (2006) Ethanol affects the generation, cosignaling molecule expression, and function of plasmacytoid and myeloid dendritic cell subsets in vitro and in vivo. J. Leukoc. Biol. 79, 941–953.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Legge, K.L., Schlueter, A.J. (2008). Dendritic Cells in Chronic In Vivo Ethanol Exposure Models. In: Nagy, L.E. (eds) Alcohol. Methods in Molecular Biology™, vol 447. Humana Press. https://doi.org/10.1007/978-1-59745-242-7_16
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DOI: https://doi.org/10.1007/978-1-59745-242-7_16
Publisher Name: Humana Press
Print ISBN: 978-1-58829-906-2
Online ISBN: 978-1-59745-242-7
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