Summary
Acute alcohol administration has minimal effects on basal immune function. However, when the immune system is challenged, acute alcohol administration alters the immune system's response. In the first 3 h after infection or traumatic injury, the presence of alcohol is associated with a decreased inflammatory response. This defect lasts long after the alcohol is cleared. Conversely, by 48 h after traumatic injury, the presence of alcohol is associated with a heightened inflammatory response. Aside from its in vivo actions, systemic administration of alcohol also alters the ex vivo response of immune cells, resulting in a decreased production of multiple cytokines after stimulation by lipopolysaccharide, concanavilin A, zymosan, and CpG DNA. Here, we describe a standardized model of acute administration of ethanol to mice used to study both the invivo and ex vivo responses of immune cells to ethanol.
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Acknowledgments
The authors thank Luis Ramirez and Dr. Mashkoor A. Choudhry for thoughtful discussions. This work was supported by the National Institutes of Health R01 AA12034–04 (EJK), the Ralph and Marion C. Falk Foundation, and the Illinois Excellence in Academic Medicine Grant.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Plackett, T.P., Kovacs, E.J. (2008). Acute Models of Ethanol Exposure to Mice. In: Nagy, L.E. (eds) Alcohol. Methods in Molecular Biology™, vol 447. Humana Press. https://doi.org/10.1007/978-1-59745-242-7_1
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DOI: https://doi.org/10.1007/978-1-59745-242-7_1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-906-2
Online ISBN: 978-1-59745-242-7
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