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Fungal-Grade Reagents and Materials for Molecular Analysis

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Human Fungal Pathogen Identification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1508))

Abstract

Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.

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Acknowledgement

The work was supported by the European Union, FP7-HEALTH-2013-INNOVATION-1, project ID 602125: Optimized Diagnostics for Improved Treatment Stratification in Invasive Fungal Diseases (FUNGITECT).

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Authors and Affiliations

  1. Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, 28359, Germany

    Michael G. Lorenz, Michael Lustig & Marina Linow

Authors
  1. Michael G. Lorenz
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  2. Michael Lustig
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  3. Marina Linow
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Corresponding author

Correspondence to Michael G. Lorenz .

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Editors and Affiliations

  1. Children’s Cancer Research Institute (CCRI), St.Anna Kinderkrebsforschung, Vienna, Austria

    Thomas Lion

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Lorenz, M.G., Lustig, M., Linow, M. (2017). Fungal-Grade Reagents and Materials for Molecular Analysis. In: Lion, T. (eds) Human Fungal Pathogen Identification. Methods in Molecular Biology, vol 1508. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6515-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6515-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6513-7

  • Online ISBN: 978-1-4939-6515-1

  • eBook Packages: Springer Protocols

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