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Isolation of Nucleic Acids for Fungal Diagnosis

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Human Fungal Pathogen Identification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1508))

Abstract

PCR can aid in the diagnosis of invasive fungal disease (IFD). While the large number of “in-house” methodologies drives technological diversity, providing robustness, they make it difficult to identify optimal strategies, limiting standardization, and widespread acceptance. No matter how efficient, PCR utility will be limited by the quality of extracted nucleic acid. This chapter highlights benefits and limitations affecting the nucleic acid extraction process, before focusing on recent recommendations that through multicenter evaluation have provided optimal and standardized methodology.

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Authors and Affiliations

  1. Public Health Wales Microbiology Cardiff, University Hospital of Wales, Heath Park, Cardiff, CF14 4XW, UK

    P. Lewis White & Rosemary A. Barnes

  2. Department of Infection and Immunity, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK

    Rosemary A. Barnes

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  1. P. Lewis White
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Correspondence to P. Lewis White .

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  1. Children’s Cancer Research Institute (CCRI), St.Anna Kinderkrebsforschung, Vienna, Austria

    Thomas Lion

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White, P.L., Barnes, R.A. (2017). Isolation of Nucleic Acids for Fungal Diagnosis. In: Lion, T. (eds) Human Fungal Pathogen Identification. Methods in Molecular Biology, vol 1508. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6515-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6515-1_12

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