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Planta Med 2010; 76(4): 339-344
DOI: 10.1055/s-0029-1186144
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

The Effects of the Diterpenes Isolated from the Brazilian Brown Algae Dictyota pfaffii and Dictyota menstrualis against the Herpes Simplex Type-1 Replicative Cycle

Juliana L. Abrantes1 , Jussara Barbosa2 , Diana Cavalcanti2 , Renato C. Pereira2 , Carlos L. Frederico Fontes1 , Valeria L. Teixeira2 , Thiago L. Moreno Souza4 [*] , Izabel C. P. Paixão3 [*]
  • 1Laboratório de Estrutura e Regulação de Proteínas, Programa de Pós-Graduação em Química Biológica, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
  • 2Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brasil
  • 3Laboratório de Virologia Molecular, Departamento de Biologia Celular e Molecular, Programa de Pós-Graduação em Neuroimunologia, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, Brasil
  • 4Laboratório de Vírus Respiratórios e do Sarampo, Laboratório de Referência para Influenza e Viroses Exantemáticas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
Further Information

Publication History

received April 10, 2009 revised August 9, 2009

accepted August 19, 2009

Publication Date:
17 September 2009 (online)

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Abstract

We describe in this paper that the diterpenes 8,10,18-trihydroxy-2,6-dolabelladiene (1) and (6R)-6-hydroxydichotoma-4,14-diene-1,17-dial (2), isolated from the marine algae Dictyota pfaffii and D. menstrualis, respectively, inhibited HSV-1 infection in Vero cells. We initially observed that compounds 1 and 2 inhibited HSV-1 replication in a dose-dependent manner, resulting in EC50 values of 5.10 and 5.90 µM, respectively, for a multiplicity of infection (MOI) of 5. Moreover, the concentration required to inhibit HSV-1 replication was not cytotoxic, resulting in good selective index (SI) values. Next, we found that compound 1 sustained its anti-herpetic activity even when added to HSV-1-infected cells at 6 h after infection, while compound 2 sustained its activity for up to 3 h after infection, suggesting that these compounds inhibit initial events during HSV-1 replication. We also observed that both compounds were incapable of impairing HSV-1 adsorption and penetration. In addition, the tested molecules could decrease the contents of some HSV-1 early proteins, such as UL-8, RL-1, UL-12, UL-30 and UL-9. Our results suggest that the structures of compounds 1 and 2, Brazilian brown algae diterpenes, might be promising for future antiviral design.

Key words

Dictyotaceae - Dictyota menstrualis - Dictyota pfaffii - marine algae - diterpenes - antiviral - HSV‐1

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1 Both authors contributed equally to this paper.

BSc, MSc Juliana Lourenço Abrantes

Instituto de Bioquímica Médica
Cidade Universitária
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