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DOI: 10.1055/s-0029-1186144
© Georg Thieme Verlag KG Stuttgart · New York
The Effects of the Diterpenes Isolated from the Brazilian Brown Algae Dictyota pfaffii and Dictyota menstrualis against the Herpes Simplex Type-1 Replicative Cycle
Publication History
received April 10, 2009
revised August 9, 2009
accepted August 19, 2009
Publication Date:
17 September 2009 (online)
Abstract
We describe in this paper that the diterpenes 8,10,18-trihydroxy-2,6-dolabelladiene (1) and (6R)-6-hydroxydichotoma-4,14-diene-1,17-dial (2), isolated from the marine algae Dictyota pfaffii and D. menstrualis, respectively, inhibited HSV-1 infection in Vero cells. We initially observed that compounds 1 and 2 inhibited HSV-1 replication in a dose-dependent manner, resulting in EC50 values of 5.10 and 5.90 µM, respectively, for a multiplicity of infection (MOI) of 5. Moreover, the concentration required to inhibit HSV-1 replication was not cytotoxic, resulting in good selective index (SI) values. Next, we found that compound 1 sustained its anti-herpetic activity even when added to HSV-1-infected cells at 6 h after infection, while compound 2 sustained its activity for up to 3 h after infection, suggesting that these compounds inhibit initial events during HSV-1 replication. We also observed that both compounds were incapable of impairing HSV-1 adsorption and penetration. In addition, the tested molecules could decrease the contents of some HSV-1 early proteins, such as UL-8, RL-1, UL-12, UL-30 and UL-9. Our results suggest that the structures of compounds 1 and 2, Brazilian brown algae diterpenes, might be promising for future antiviral design.
Key words
Dictyotaceae - Dictyota menstrualis - Dictyota pfaffii - marine algae - diterpenes - antiviral - HSV‐1
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1 Both authors contributed equally to this paper.
BSc, MSc Juliana Lourenço Abrantes
Instituto de Bioquímica Médica
Cidade Universitária
Ilha do Fundão
Centro de Ciências da Saúde (CCS)
Bloco H, segundo andar, sala 26
Rua Cesar Pernetta 400
CEP 21941–590, Rio de Janeiro
Brasil
Phone: + 55 21 25 62 67 84
Fax: + 55 21 25 62 67 84
Email: abrantes@bioqmed.ufrj.br