Adrenaline-Induced Chronic Ocular Hypertension in Adult Rabbits

 

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Zusammenfassung

Hintergrund: Beschreibung eines neuen Tiermodells zum besseren Verständnis der pathophysiologischen Mechanismen beim Glaukom. Material und Methoden: Von 40 weißen Riesenkaninchen wurden 30 Tiere behandelt, 10 Tiere dienten als Kontrollgruppe. Die Kaninchen wurden 3 Monate lang wiederholt mit Boli von Adrenalin-Hydrochlorid (0,1 mL einer 0,1 % Lösung) behandelt, welche ihnen in die Ohrvenen injiziert wurden. Die Kontrollgruppe erhielt statt Adrenalin physiologische Kochsalzlösung. Gemessen wurden der Augendruck (IOD) und die Abflussrate des Kammerwassers bei Beginn, während und nach der Behandlung (nach 4 – 6 Monaten, 7 – 9 Monaten und 10 – 12 Monaten). Ergebnisse: Im Vergleich zur Kontrollgruppe zeigten die mit Adrenalin behandelten Tiere während der Behandlung eine signifikante Steigerung des Augendrucks um 25 % und 12 Monate nach der Behandlung um 57 %. Ferner zeigte der Vergleich, dass die Abflussrate des Kammerwassers bei den behandelten Tieren um 16,5 % während der Behandlung zunahm und dann kontinuierlich um 60 % nach der Behandlung sank. Schlussfolgerungen: Das vorliegende Tiermodell dürfte für künftige Untersuchungen des Pathomechanismus beim Glaukom wertvoll sein.

Abstract

Background: The aim of this study was to develop a new animal model to enhance our understanding of the biological pathomechanisms involved in glaucoma. Materials and Methods: Forty white giant rabbits were divided into a treated (N = 30) and a control group (N = 10). Boli of adrenaline hydrochloride (0.1 mL 0.1 % solution) were repeatedly injected into the veins of the ears of the rabbits and physiological saline in the control group, respectively, for three months. Intraocular pressure (IOP) and outflow facility of the aqueous humour were measured prior to, during and after treatment (4 – 6 months, 7 – 9 months, 10 – 12 months). Results: In comparison to the control group, the adrenaline-treated group showed a significant increase in IOP both during treatment (25 %) and 12 months after treatment (57 %). Comparative analysis further showed that the aqueous humour outflow facility of the treated group increased by 16.5 % during the treatment, and showed a continuous decrease of 60 % after treatment. Conclusion: This rabbit model could be useful for further investigations of the pathomechanisms involved in glaucoma.

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Prof. Dr. Peter Meyer

Augenklinik, Universitätsspital Basel

Mittlere Straße 91

4031 Basel

Switzerland

Email: meyerpe@uhbs.ch