Folia Parasitologica 49[2] 153-159 (2002) | DOI: 10.14411/fp.2002.027

Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes

Jitka Pechová1, Gabriela Štěpánová1, Lubomír Kovář2, Jan Kopecký1
1 Institute of Parasitology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
2 Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic

Saliva-activated transmission of Borrelia afzelii Canica, Nato, du Merle, Mazie, Baranton et Postic, 1993 was demonstrated using salivary gland extract (SGE) from Ixodes ricinus (L., 1758) ticks and C3H mice. Injection of Borrelia spirochaetes together with SGE increased the level of bacteraemia and accelerated the appearance of bacteria in the urinary bladder, compared with the injection of spirochaetes alone. More I. ricinus nymphs became infected when feeding on mice inoculated with B. afzelii plus SGE. Analysis of cytokines produced by cells of draining lymph nodes from SGE-treated mice showed a suppression of proinflammatory cytokines IFN-γ, IL-6 and GM-CSF following a transient upregulation in comparison with the control mice infected without SGE.

Keywords: Ixodes ricinus, salivary gland extract, Borrelia afzelii, transmission

Received: August 20, 2001; Accepted: November 15, 2001; Published: June 1, 2002  Show citation

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Pechová, J., Štěpánová, G., Kovář, L., & Kopecký, J. (2002). Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Folia Parasitologica, 49(2), 153-159. doi: 10.14411/fp.2002.027
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