Abstract
Carcinoma of the human uterine cervix has been associated with several infectious agents1–5 including papillomaviruses6,7. Papillomavirus group-specific antigen (GSA)8,9 and viral particles10 have been demonstrated in human condylomata acuminata (CA) and flat warts of the uterine cervix. Cell alterations consisting of nuclear enlargement, hyperchromasia, irregularity, binucleation and cytoplasmic clearing (koilocytosis) are often interpreted as mild to moderate dysplasia11. Present evidence that human papillomavirus (HPV) is responsible for the development of these lesions relies on the association of GSA7 and virus particles8 in the affected tissue, fulfilling the first two of Koch's postulates12. Direct proof of an aetiological relationship, however, requires induction of the CA change in normal, human uterine cervix after exposure to papillomavirus. Infecting human subjects with HPV is ethically unacceptable and no satisfactory alternative systems have been defined. Also, human cell cultures do not support growth or transformation by HPV. Here we report the first demonstration of the morphological transformation of human tissues with a human papillomavirus under controlled, experimental conditions. ‘Transformation’ is used here in its literal sense to refer to a heritable morphological alteration in the.appearance of the cells. The use of this term does not indicate that the changes described are neoplastic, but they are identical to the dysplastic changes found in biopsies of uterine cervical CA. Our results demonstrate the direct involvement of CA virus in dysplastic change of human cervical tissue and indicate that the experimental system described may be useful in elucidating the contribution of human papillomaviruses to the pathogenesis of human cervical cancer.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kessler, I. I. Cancer Res. 36, 783–791 (1973).
Nahmias, A., Naib, Z. M. & Josey, W. Cancer Res. 34, 1111–1117 (1974).
Schechter, J. et al. J. Am. med. Ass. 248, 2134–2142 (1982).
Singer, A., Reid, R. & Coppelson, M. A. Am. J. Obstet. Gynec. 126, 110–116 (1976).
Heins, H. C., Dennis, E. J. & Pratt-Thomas, H. R. Am. J. Obstet. Gynec. 76, 726–735 (1958).
zur-Hausen, H. Curr. Topics Microbiol. Immun. 78, 1–30 (1977).
Pfister, H. Rev. Physiol. Biochem. Pharmac. 99, 111–181 (1984).
Jenson, A. B. et al. J. natn. Cancer Inst. 64, 495–500 (1980).
Kurman, R. J., Jenson, A. B. & Lancaster, W. D. Am. J. surg. Path. 7, 39–52 (1983).
Della Torre, G., Pilotti, S., De Palo, G. & Rilke, F. Tumori 64, 549–553 (1978).
Meisels, A. & Fortin, R. Acta cytol. 20, 505–509 (1976).
Joklik, W. K., Willett, H. P. & Amos, D. B. Zinsser Microbiology 17th edn, 6 (Appleton-Century-Crofts, New York, 1980).
Kreider, J. W., Bartlett, G. L. & Sharkey, F. E. Cancer Res. 39, 273–276 (1979).
Duff, R. & Rapp, F. J. Virol. 8, 469–477 (1971).
Gissman, L. et al. Proc. natn. Acad. Sci. U.S.A. 80, 560–563 (1983).
Bosshart, M. et al. EMBO J. 3, 1151–1157 (1984).
Durst, M., Gissman, L., Ikenberg, H. & zur Hausen, H. Proc. natn. Acad. Sci. U.S.A. 80, 3812–3815 (1983).
Mandel, M. & Higa, A. J. molec. Biol. 53, 154–162 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kreider, J., Howett, M., Wolfe, S. et al. Morphological transformation in vivo of human uterine cervix with papillomavirus from condylomata acuminata. Nature 317, 639–641 (1985). https://doi.org/10.1038/317639a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/317639a0
This article is cited by
-
The importance of HPV vaccination today
memo - Magazine of European Medical Oncology (2014)
-
Contemporary Theories of Cervical Carcinogenesis: The Virus, the Host, and the Stem Cell
Modern Pathology (2000)
-
Multiplicity of uses of monoclonal antibodies that define papillomavirus linear immunodominant epitopes
Immunologic Research (1997)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.