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High FLT3 expression and IL10 (G1082A) polymorphism in poor overall survival in calla acute lymphoblastic leukemia

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Abstract

Patients with acute lymphoblastic leukemia presenting the immunophenotypic marker CD10+ (calla), usually has treatment profile good. The FLT3 molecular marker is listed as a prognostic factor, an important leukaemogenic marker in acute leukemias, also the polymorphism (G1082A) of the IL10 interleukin can to present pleiotropic effects in many diseases and could is associated to development of ALL. However, the FLT3 expression is variability among patients with calla-ALL. The aim of this study was to determine the FLT3 expression, to associate with the genotypes and allelic of G1082A (IL10) in 50 patients with calla-ALL and assess the overall survival at 98 months follow-up. The expression was assessed by quantitative real time PCR (RT-PCR), the G1082A polymorphism was identified by allele-specific PCR and for immunophenotypic classification was used specific markers of B lineage-calla. We observed that patients who died showed higher FLT3 expression (p = 0.005), worse survival (p = 0.0137) and the IL10G allele may favor the survival, because the IL10 GG and IL10 GA genotypes showed a low FLT3 expression (p < 0.05).

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Acknowledgments

We thank the FACEPE (Foundation for assistance to science and technology of the state of Pernambuco), UNIPECLIN (Clinical Research Unit) and CAPES (Coordination for Improvement in Higher Education). We thank the support of Dr. Ednalva Pereira Leite (CEONHPE) and Dr. Vera Lúcia Lins de Morais (CEONHPE) for clinical competence.

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Authors and Affiliations

  1. Pediatric Hematology Oncology Center (CEONHPE/UPE), Recife, PE, Brazil

    Dayse Maria Vasconcelos de Deus & Maria Tereza Cartaxo Muniz

  2. Federal Rural University of Pernambuco (UFRPE), Recife, PE, Brazil

    Paulo Roberto Eleutério de Souza

  3. University of Pernambuco (UPE), Recife, PE, Brazil

    Maria Tereza Cartaxo Muniz

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  1. Dayse Maria Vasconcelos de Deus
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  2. Paulo Roberto Eleutério de Souza
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  3. Maria Tereza Cartaxo Muniz
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Correspondence to Dayse Maria Vasconcelos de Deus.

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de Deus, D.M.V., de Souza, P.R.E. & Muniz, M.T.C. High FLT3 expression and IL10 (G1082A) polymorphism in poor overall survival in calla acute lymphoblastic leukemia. Mol Biol Rep 40, 1609–1613 (2013). https://doi.org/10.1007/s11033-012-2209-4

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  • DOI: https://doi.org/10.1007/s11033-012-2209-4

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