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Medical ozone therapy decreases postoperative uterine adhesion formation in rats

  • General Gynecology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Various studies have been performed to find out novel treatment strategies to prevent postoperative adhesion formation. Ozone therapy (OT) is shown to reduce inflammation in several pathological conditions. Therefore, we aimed to evaluate the efficacy of OT in a rat model of experimental uterine adhesion (EUA).

Methods

Thirty female Wistar rats (200–250 g) were divided into three groups: sham, EUA and EUA+OT. EUA and EUA+OT groups were subjected to the postoperative adhesion procedure by bipolar coagulation on the uterine horns and corresponding pelvic sidewall parietal peritoneum. EUA+OT group received 0.7 mg/kg daily single dose for 3 days of ozone/oxygen mixture intraperitoneally after adhesion induction. All animals were killed on the 7th day and uterine adhesions were scored. Uterine tissues and peritoneal washing fluid were harvested for all analyses.

Results

Uterine malondialdehyde levels in the EUA group were significantly higher compared to the other groups. However, in the EUA group, uterine superoxide dismutase and glutathione peroxidase activities were lower than in other groups. Peritoneal fluid TNF-α levels were found to be significantly different for all groups (p < 0.001). Macroscopic total adhesion score was significantly higher in the EUA group compared to the other groups (p < 0.001). But, total score in the EUA+OT group was lower than in the EUA group (p = 0.006).

Conclusions

Medical OT prevents postoperative uterine adhesions by modulating TNF-α levels and oxidative/antioxidative status in an experimental uterine adhesion model.

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Author information

Authors and Affiliations

  1. Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey

    Bulent Uysal & Ahmet Korkmaz

  2. Department of Pediatric Surgery, Gulhane Military Medical Academy, Etlik, Ankara, 06018, Turkey

    Suzi Demirbag, Ahmet Guven & Ilhami Sürer

  3. Department of General Surgery, Elazig Military Hospital, Elazig, Turkey

    Yavuz Poyrazoglu

  4. Department of Clinical Biochemistry, Gulhane Military Medical Academy, Ankara, Turkey

    Tuncer Cayci

  5. Department of Obstetrics and Gynecology, Yeditepe University, Istanbul, Turkey

    Narter Yesildaglar

Authors
  1. Bulent Uysal
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  2. Suzi Demirbag
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  3. Yavuz Poyrazoglu
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Correspondence to Suzi Demirbag.

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Uysal, B., Demirbag, S., Poyrazoglu, Y. et al. Medical ozone therapy decreases postoperative uterine adhesion formation in rats. Arch Gynecol Obstet 286, 1201–1207 (2012). https://doi.org/10.1007/s00404-012-2435-y

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  • DOI: https://doi.org/10.1007/s00404-012-2435-y

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