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Aurora A Kinase as a diagnostic urinary marker for urothelial bladder cancer

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Abstract

Objective

To evaluate urinary Aurora A Kinase (AURKA) mRNA expression as a diagnostic biomarker for urothelial bladder cancer (UBC).

Methods

One hundred and eighty-eight urine samples from patients with UBC (n = 122) and controls with hematuria (n = 66) were investigated. AURKA expression was quantified using real-time PCR and compared with voided urinary cytology. Associations with stage and grade were assessed. The area under curve was used to quantify the predictive accuracy (PA).

Results

The sensitivity and the specificity of AURKA for UBC were 83.6 and 65.2 %, respectively (PA = 74.4 %). Among those with detectable AURKA, the quantity of expression was similar in cases and controls. Compared with Ta, tumors staged T1 and T2 showed a 9.31-fold and 4.78-fold increased AURKA expression (p = 0.034), respectively. Further, high-grade tumors showed 5.33-fold higher expression levels than low-grade tumors (p = 0.031). AURKA and urinary cytology showed similar overall PA for UBC detection (74.4 vs. 72.1 %, p = 0.588). For low-grade tumors, AURKA was more accurate (72.5 vs. 59.0 %, p = 0.004), while cytology was more accurate for high-grade lesions (76.8 vs. 89.1 %, p = 0.011).

Conclusions

In patients with hematuria, AURKA is associated with the presence and grade of UBC, suggesting a role as diagnostic and prognostic biomarker. As AURKA is more accurate in low-grade tumors but less accurate in high-grade tumors than urinary cytology, both could be complementary in detecting UBC.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

The study was approved by the local ethics committee and was therefore performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

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Authors and Affiliations

  1. Department of Urology, Vienna General Hospital, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria

    Michela de Martino, Shahrokh F. Shariat, Sebastian L. Hofbauer, Ilaria Lucca, Christopher Taus & Tobias Klatte

  2. Clinical Institute of Pathology, Vienna General Hospital, Medical University of Vienna, Vienna, Austria

    Helene G. Wiener, Andrea Haitel & Martin Susani

Authors
  1. Michela de Martino
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  2. Shahrokh F. Shariat
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  3. Sebastian L. Hofbauer
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  4. Ilaria Lucca
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  6. Helene G. Wiener
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  9. Tobias Klatte
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Correspondence to Tobias Klatte.

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de Martino, M., Shariat, S.F., Hofbauer, S.L. et al. Aurora A Kinase as a diagnostic urinary marker for urothelial bladder cancer. World J Urol 33, 105–110 (2015). https://doi.org/10.1007/s00345-014-1267-8

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  • DOI: https://doi.org/10.1007/s00345-014-1267-8

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