Abstract
The prevalence of celiac disease (CD) was determined in healthy blood donors and in high-risk groups of adults (a total of 1835 adults—randomly selected 1312 healthy blood donors, 102 patients with primary osteoporosis, 58 patients with autoimmune diseases and 365 infertile women). It was calculated on the basis of a two-step serologic screening method—in the first step IgA and IgG antigliadin antibodies (AGA) and IgA anti-γ-glutamyltransferase (‘transglutaminase’) antibodies (ATG) were estimated, in the second step sera positive for IgA AGA and/or IgA ATG were examined for antiendomysial IgA (AEA) antibodies. Immunoenzymic assay (ELISA) was used for determining of AGA and ATG antibodies; immunoflurescence method, performed on human umbilical cord tissue, was used for assaying of AEA antibodies. Total serum IgA level in only IgG AGA positive subjects was measured by routine turbidimetric method. 0.45% of healthy blood donors, 0.98% of osteoporotic patients, 2.7% of patients suffering from autoimmune disease and 1.13% of women with infertility considered as immunologically mediated were found to be positive in both steps of serologic screening (AGA and/or ATG and antiendomysium positive). The presumed high prevalence of seropositivity for CD in apparently healthy Czech adult population was confirmed. In the high-risk groups, the prevalence of seropositivity for CD was approximately 2–4 times higher than in healthy blood donors. The real prevalence of CD in the tested groups, however, can be estimated after performing small intestinal biopsy in the seropositive patients.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ascher H., Hahn-Zoric M., Hanson L.A., Kilander A.F., Nilsson L.A., Tlaskalová H.: Value of the serological markers for clinical diagnosis and population studies of celiac disease.Scand. J. Gastroenterol. 31, 61–67 (1996).
Ashabani A., Abushofa U., Abusrevill S, Abdelazez M., Tučková L., Tlaskalová-Hogenová H.: The prevalence of celiac disease in Libyan children with insulin dependent diabetes mellitus.Diabetes Metab. Res. Rev., in press (2002).
Carlsson A.K., Axelsson I.E.M., Borulf S.K., Bredberg A.C.A., Ivarsson S.A.: Serological screening for celiac disease in healthy 2.5-year-old children in Sweden.Pediatrics 107, 42–45 (2001).
Catassi C., Fabini E., Rätsch I.M., Rossni M., Bordicchia F., Coppa G.V., Giorgi P.L.: Celiac disease in the year 2000; exploring the iceberg.Lancet 343, 200–203 (1994).
Catassi C., Fabiani E., Rätsch I.M., Coppa G.V., Giorgi P.L., Pierdomenico R., Alessandrini S., Iwanejko G., Domenici R., Mei E., Miano A., Marani M., Bottaro G., Spina M., Dotti M., Montanelli A., Barbato M., Viola F., Lazzari R., Vallini M., Guariso G., Plebani M., Cataldo F., Traveso G., Ughi C., Chiravalotti G., Baldassarre M., Scarcella P., Bascietto F., Ceglie L., Valenti A., Paolucci P., Caradonna M., Bravi E., Ventura A.: The celiac iceberg in Italy. A multicentre antigliadin antibodies screening for celiac disease in school-age subjects.Acta Paediatr. 412 (Suppl.), 29–35 (1996).
Collin P., Reunala T., Pukkala E., Laippala P., Keyrilainen O., Pasternack A.: Celiac disease—associated disorders and survival.Gut 35, 1213–1218 (1994).
Cronin C.C., Shanahan F.: Insulin dependent diabetes mellitus and celiac disease.Lancet 349, 1096 (1997).
De Vitis I., Ghirlanda G., Gasbarrini G.: Prevalence of celiac disease in type 1 diabetes: a multicenter study.Acta Paediatr. 412 (Suppl.), 56–57 (1996).
Dickey W., Hughes D.F., McMillan S.A.: Reliance on serum endomysial antibody testing underestimates the true prevalence of celiac disease by one fifth.Scand. J. Gastroenterol. 35, 181–183 (2000).
Dieterich W., Ehnis T., Bauer M., Donner P., Volta U., Riecken E., Schuppan D.: Identification of tissue transglutaminase as the autoantigen of celiac disease.Nat. Med. 3, 797–801 (1997).
Fasano A., Catassi C.: Current approaches to diagnosis and treatment of celiac disease: an evdving spectrum.Gastroenterology 120, 636–651 (2001).
Feighery C.: Fortnigthly review: celiac disease.Brit. Med. J. 24, 236–239 (1999).
Gandolfi L., Pratesi R., Cordoba J.C.M., Tauil P.L., Gasparin M., Catassi C.: Prevalence of celiac disease among blood donors in Brazil.Am. J. Gastroenterol. 95, 689–692 (2000).
Holmes G.T.K.T., Prior P., Lane M.R., Pope D., Allan R.N.: Malignancy in celiac disease. Effect of gluten-free diet.Gut 3, 333–338 (1989).
Johnston S.D., Watson R.G.P., McMillan S.A.: Prevalence of celiac disease in Northern Ireland.Lancet 350, 1370 (1997).
Johnston S.D., Watson R.G.P., McMillan S.A., Sloan J., Love A.H.: Celiac disease detected by screening in not silent—simply unrecognized.Quart. J. Med. 91, 853–860 (1998).
Landiser B., Rossipal E., Pittschieler K.: Endomysium antibodies in celiac disease: an improved method.Gut 35, 581–591 (1994).
Logan R.F.A.: Epidemiology of celiac disease, pp. 192–194 in M.N. Marsh (Ed.):Celiac disease. Blackwell Scientific, Oxford (UK) 1992.
Marsh M.N.: Mucosal pathology in gluten sensitivity, pp. 136–191 in M.N. Marsh (Ed.):Celiac Disease. Blackwell Scientific, Oxford (UK) 1992.
Not T., Horvath K., Hill I.D.: Celiac disease risk in the USA: high prevalence of antiendomysial antibodies in healthy blood donors.Scand. J. Gastroenterol. 33, 494–498 (1998).
Riestra S., Fernandez E., Rodrigo L., Garcia S., Ocio G.: Prevalence of celiac disease in the general population of Northern Spain. Strategies of serological screening.Scand. J. Gastroenterol. 35, 398–402 (2000).
Sánchez D., Tučková L., Šebo P., Michalak M., Whelan A., Šterzl I., Jelínková L., Havrdová E., Imramovská M., Beneš Z., Krupičková S., Tlaskalová-Hogenová H.: Occurence of IgA and IgG autoantibodies to calreticulin in celiac disease and various autoimmune diseases.J. Autoimmun. 15, 441–449 (2000).
Stern M.: Comparative evaluation of serologic tests for celiac disease: a European initiative toward standardization.J. Ped. Gastroenterol. Nutr. 31, 513–519 (2000).
Stoyanov S., Tlaskalová-Hogenová H., Kocna P., Krištofová H., Frič P., Hekkens W.T.J.M.: Monoclonal antibodies reacting with gliadin as tools for assessing antigenic structure responsible for exacerbation of celiac disease.Immunol. Lett. 17, 335–338 (1988).
Šumník Z., Koloušková S., Cinek O., Kotalová R., Vavřinec J., Šnajderová M.: HLA-DQA1*05-DQB1*0201 positivity predisposes to celiac disease in Czech diabetic children.Acta Paediatr. 89, 1426–1430 (2000).
Tlaskalová-Hogenová H., Štěpánková R., Tučková L., Farré M.A., Funda D.P., Verdú E.F., Šinkora J., Hudcovič T., Řeháková Z., Cukrowska B., Kozáková H., Prokešová L.: Autoimmunity, immunodeficiency and mucosal infections: chronic intestinal inflammation as sensitive indicator of immunoregulatory defects in response to normal luminal microflora.Folia Microbiol. 43, 545–550 (1998).
Tlaskalová-Hogenová H., Tučková L., Pospíšil M., Štěpánková R.: Malignancy in celiac disease and dermatitis herpetiformis, pp. 105–110 in Y. Shoenfeld, M.E. Gershwin (Eds):Cancer and Autoimmunity. Elsevier Science Publishers, Amsterdam (The Netherlands) 2000.
Trevisiol C., Not T., Berti I., Buratti E., Citta A., Neri E., Torre G., Martelossi S., Tommasini A., Alu A., Barillari G., Facchini S., Ventura A.: Screening for celiac disease in healthy blood donors at two immuno-transfusion centers in North-East Italy.Ital. J. Gastroenterol. Hepatol. 31, 584–586 (1999).
Tučková L., Karská K., Walters J.R.F., Michalak M., Rossmann P., Krupičková S., Verdu E.F., Saalman R., Hanson L.A., Tlaskalová-Hogenova H.: Anti-gliadin antibodies in patients with celiac disease cross-react with enterocytes and human calreticulin.Clin. Immunol. Immunopathol. 85, 289–296 (1997).
Valdimirasson T., Toss G., Ross I., Lofman O., Strom M.: Bone mineral density in celiac disease.Scand. J. Gastoenterol. 29, 457–461 (1994).
Volta U., Molinaro N., de Franceschi I., Fratangelo D., Bianchi F.B.: IgA anti-endomysium antibodies on human umbilical cord tissue for celiac disease screening, save both money and monkeys.Diag. Dis. Sci. 40, 1920–1925 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vančíková, Z., Chlumecký, V., Sokol, D. et al. The serologic screening for celiac disease in the general population (blood donors) and in some high-risk groups of adults (patients with autoimmune diseases, osteoporosis and infertility) in the Czech Republic. Folia Microbiol 47, 753–758 (2002). https://doi.org/10.1007/BF02818684
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02818684