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Modulatory effects of the human heat shock protein 70 on DNA vaccination

  • Original Paper
  • Published:
Journal of Biomedical Science

Abstract

DNA vaccination with the plasmid expressing Japanese encephalitis virus (JEV) nonstructural protein 1 (pJNS1) has been shown to induce effective immunity against JEV infection. To further increase the efficacy of pJNS1 DNA vaccination, we coinjected pJNS1 with a plasmid that expresses heat shock protein 70.1 (pHSP70.1) into mice. We found that coinjection of pHSP70.1 enhanced both T cell proliferation and cytotoxic effects, but not the antibody response to JEV. Moreover, mice immunized with both pHSP70.1 and pJNS1 were resistant to lethal challenges of JEV, indicating that the protective immunity against JEV is not decreased, in spite of the low antibody titer via the immunization of pHSP70.1. Since DNA vaccination administered by pJNS1 did not elicit strong cellular immunity in our previous study, the administration of pHSP70.1 apparently could be used as an adjuvant to enhance cell-mediated immunity in this model system. Thus, coadministration of pHSP70.1 DNA with plasmid DNA encoding tumor- or virus-specific antigens might be very useful in the treatment of cancers and other infectious diseases.

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Authors and Affiliations

  1. Immunology Research Center and Department of Microbiology and Immunology, National Yang-Ming University School of Medicine, Taiwan, Republic of China

    Wei-Hsu Chen & Shie-Liang Hsieh MD, PhD

  2. Institute of Biomedical Sciences, Academia Sinica, Taiwan, Republic of China

    Yi-Ling Lin

  3. Department of Microbiology and Immunology, National Defense Medical School, Taipei, Taiwan, Republic of China

    Ching-Len Liao

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  1. Wei-Hsu Chen
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  2. Yi-Ling Lin
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  4. Shie-Liang Hsieh MD, PhD
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Chen, WH., Lin, YL., Liao, CL. et al. Modulatory effects of the human heat shock protein 70 on DNA vaccination. J Biomed Sci 7, 412–419 (2000). https://doi.org/10.1007/BF02255816

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  • DOI: https://doi.org/10.1007/BF02255816

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