Abstract
In this pilot study we changed several pre-analytical variables of bone marrow trephine biopsy processing with the task to achieve not only a preservation of morphology and antigens but also of nucleic acids. The changes involved employment of a newly established decalcification solution in conjunction with a short fixation time (2 h after receiving the specimens) and performance of decalcification at 37 °C. The comparison of the obtained results from three specimens with those of our routinely established protocol unequivocally revealed that the novel decalcification solution results in a superior preservation of nucleic acids, with only slight differences in preservation of morphology and cellular antigens. These encouraging results imply that this novel decalcification solution will allow a widely accepted standardization of bone marrow trephine biopsy processing.
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Acknowledgments
The expert technical assistance of Vera Arnemann, Erika Berg, Franziska Gocht, Hedwig Lammert, Anita Lehman, Stefanie Mende and Hans-Henning Müller is gratefully acknowledged.
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Anagnostopoulos, I., Lenze, D., Hummel, M., Dietel, M., Jöhrens, K. (2015). Bone Marrow Work-up: Report of a Pilot Study. In: Dietel, M., Wittekind, C., Bussolati, G., von Winterfeld, M. (eds) Pre-Analytics of Pathological Specimens in Oncology. Recent Results in Cancer Research, vol 199. Springer, Cham. https://doi.org/10.1007/978-3-319-13957-9_10
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DOI: https://doi.org/10.1007/978-3-319-13957-9_10
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