What you will learn
After finishing this chapter, you will have a basic overview of the individual experimental work steps regarding the NGS library preparation workflow, the connection between the scientific or clinical question and the choice of the corresponding library preparation. For this purpose, the technical and molecular biological relevance of the individual work steps is briefly described.
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Acknowledgements
We are grateful to Dr. Ines Böhme (Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–Nürnberg, Erlangen, Germany) for reviewing this chapter and Alexander Oliver Matthies (Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–Nürnberg, Erlangen, Germany) for critically reading this text.
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Kappelmann-Fenzl, M. (2021). Library Construction for NGS. In: Kappelmann-Fenzl, M. (eds) Next Generation Sequencing and Data Analysis. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-62490-3_3
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DOI: https://doi.org/10.1007/978-3-030-62490-3_3
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