Abstract
The predominant signalling pathway by which hormones and neurotransmitters control the secretion of lacrimal fluids and proteins involves an elevation of cytoplasmic calcium. We have investigated this signalling system in mouse lacrimal acinar cells activated through their muscarinic cholinergic receptors. These receptors initiate signalling by activation of a phospholipase C enzyme that degrades specifically a minor membrane phospholipid, phosphatidylinositol bisphosphate.1,2 The resulting products are 1,2—diacylglycerol (DG) and inositol 1,4,5—trisphosphate (IP3); both play important roles in determining the extent and duration of lacrimal calcium signalling.
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References
Berridge MJ. Inositol trisphosphate and calcium signalling. Nature. 1993; 361: 315–325.
Putney JW Jr., Bird GSJ. The inositol phosphate-calcium signalling system in non-excitable cells. Endocr Rev. 1993; 14: 610–631.
Putney JW Jr. A model for receptor-regulated calcium entry. Cell Calcium. 1986; 7: 1–12.
Putney JW Jr. Capacitative calcium entry revisited. Cell Calcium. 1990; 11: 611–624.
Kwan CY, Takemura H, Obie JF, Thastrup O, Putney JW Jr. Effects of methacholine, thapsigargin and Lai+ on plasmalemmal and intracellular Ca2+ transport in lacrimal acinar cells. Am J Physiol. 1990;258:C1006-C1015.
Bird GSJ, Putney JW, Jr. Effect of inositol 1,3,4,5-tetrakisphosphate on inositol trisphosphate-activated Ca2+ signalling in mouse lacrimal acinar cells. J Biol Chem. 1996; 271: 6766–6770.
Bird GSJ,,Obie JF, Putney JW Jr. Functional homogeneity of the non-mitochondrial Ca2+-pool in intact mouse lacrimal acinar cells. JBiol Chem. 1992; 267: 18382–18386.
Jacob R. Calcium oscillations in electrically non-excitable cells. Biochim Biophys Acta. 1990; 1052: 427–438.
Gray PTA. Oscillations of free cytosolic calcium evoked by cholinergie and catecholaminergic agonists in rat parotid acinar cells. J Physiol. (Lond). 1988; 406: 35–53.
Yule DI, Gallacher DV. Oscillations of cytosolic calcium in single pancreatic acinar cells stimulated by acetylcholine. FEBS Lett. 1988; 239: 358–362.
Tsunoda Y, Stuenkel EL, Williams JA. Oscillatory mode of calcium signalling in rat pancreatic acinar cells. Am J Physiol. 1990; 258: C147 - C155.
Sage SO, Adams DJ, Van Breemen C. Synchronized oscillations in cytoplasmic free calcium concentration in confluent bradykinin-stimulated bovine pulmonary artery endothelial cell monolayers. J Biol Chem. 1989; 264: 6–9.
Bird GSJ, Rossier MF, Obie JF, Putney JW Jr. Sinusoidal oscillations in intracellular calcium due to negative feedback by protein kinase C. J Biol Chem. 1993; 268: 8425–8428.
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© 1998 Springer Science+Business Media New York
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Putney, J.W., Huang, Y., Bird, G.S.J. (1998). Calcium Signalling in Lacrimal Acinar Cells. In: Sullivan, D.A., Dartt, D.A., Meneray, M.A. (eds) Lacrimal Gland, Tear Film, and Dry Eye Syndromes 2. Advances in Experimental Medicine and Biology, vol 438. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5359-5_16
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DOI: https://doi.org/10.1007/978-1-4615-5359-5_16
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