Abstract
Tear lipocalins (TL) are novel members of the lipocalin family, a group of proteins that are capable of binding and transporting small hydrophobic molecules.1,2 Most lipocalins, such as retinol binding protein and pheromaxeine bind specific ligands. However,TL are capable of binding a broad array of lipid molecules.3 Cholesterol, fatty acids, phospholipids, and glycolipids are bound to TL in tears. TL are promiscuous and have been shown to bind retinol, synthetic analogs of cholesterol, fatty acids, fatty alcohols, phospholipids, glycolipids, and even environmental contaminants such as dioctyl phthalate.1,3
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References
Redl B, Holzfeind P, Lottspeich F. cDNA cloning and sequencing reveals human tear prealbumin to be a member of the lipophilic-ligand carrier protein superfamily. J Biol Chem. 1992; 267: 20282–20287.
Delaire A, Lassagne H, Gachon AMF. New members of the lipocalin family in human tear fluid. Exp Eye Res. 1992;55:645–647.
Glasgow BJ, Abduragimov AR, Farahbakhsh Z, Faull KF, Hubbell WL. Tear lipocalins bind a broad array of lipid ligands. Curr Eye Res. 1995; 14: 363–372.
Flower DR. The lipocalin protein family: Structure and function. Biochem J. 1996; 318: 1–14.
Glasgow BJ, Abduragimov AR, Yusifov TN, Faull KF, Hubbell WL, Horwitz J. Characterization of the disulfide motif in the major isoform of tear lipocalins. ARVO Abstracts. Invest Ophthalmol Vs Sci. 1996; 37: S849.
Glasgow BJ. Tissue expression of lipocalins in human lacrimal and von Ebner’s glands: Colocalization with lysozyme. Graefes Arch Clin Exp Ophthalmol. 1995; 233: 513–522.
Bozimowski D, Artiss JD, Zak B. The variable reagent blank: Protein determination as a model. J Clin Chem Clin Biochem. 1985; 23: 683–689.
Elman GL. Tissue sulfhydryl group. Arch Biochem Biophy. 1959;82:70–77.
Heller J, Horwitz J. Conformational changes following interaction between retinol isomers and human retinol-binding protein and between the retinol-binding protein and prealbumin. J Biol Chem. 1973; 248: 6308–6316.
Hubbard R, Brown PK, Bounds D. Methodology of vitamin A and visual pigments. Methods Enzymol. 1971; 18: 628–629.
Glatz JFC, Veerkamp JH. A radiochemical procedure for the assay of fatty acid binding by proteins. Anal Biochem. 1983; 132: 89–95.
Vork MM, Glatz JFC, Surtel DAM, van der Vusse GF. Assay of the binding of fatty acids by proteins: Evaluation of the Lipidex 1000 procedure. Mol Cell Biochem. 1990; 98: 111–117.
Lassagne H, Gachon AMF. Cloning of a human lacrimal lipocalin secreted in tears. Exp Eye Res. 1993; 56: 605–609.
Glasgow BJ, Heinzmann C, Kojis T, Sparkes RS, Mohandas T, Bateman JB. Assignment of tear lipocalin gene to human chromosome 9q34–9qter. Curr Eye Res. 1993; 12: 1019–1023.
Bläker M, Kock K, Ahlers C, Buck F, Schmale H. Molecular cloning of human von Ebner’s gland protein, a member of the lipocalin superfamily high expressed in lingual salivary glands. Biochim Biophys Acta. 1993; 1172: 131–137.
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Glasgow, B.J., Abduragimov, A.R., Yusifov, T.N., Gasymov, O.K. (1998). Studies of Ligand Binding and CD Analysis with Apo- and Holo-Tear Lipocalins. In: Sullivan, D.A., Dartt, D.A., Meneray, M.A. (eds) Lacrimal Gland, Tear Film, and Dry Eye Syndromes 2. Advances in Experimental Medicine and Biology, vol 438. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5359-5_14
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DOI: https://doi.org/10.1007/978-1-4615-5359-5_14
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