Abstract
Thymidine kinase is an enzyme in the pyrimidine salvage pathway that, with ATP as co-substrate, catalyzes the phosphorylation of deoxythymidine to deoxythymidine monophosphate (dTMP) which is subsequently converted to dTTP and utilized for DNA synthesis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
D.L. Coppock and A.B. Pardee, Control of thymidine kinase mRNA during cell cycle. Molecular and cellular biology. 7:2925 (1987).
B. Munch-Petersen and G. Tyrsted, Thymidine kinase isoenzymes in human acute and chronic lymphatic leukemia. Leukemia Res. 10:637 (1986).
J.L. Sherley and T.J. Kelly, Regulation of human thymidine kinase during the cell cycle. J. Biol. Chem. 263:375 (1988b).
B. Munch-Petersen, Differences in the kinetic properties of thymidine kinase isoenzymes in unstimulated and phytohemagglutinin-stimulated human lymphocytes. Mol. Cell. Biochem. 64:173 (1984).
M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quanties of protein utilizing the principle of protein-dye binding. Anal.Biochem. 72:248 (1976).
P. Chomczynski and N. Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156 (1987).
G. Gilliand, S. Perrin and F. Bunn, in: PCR Protocols. A guide to methods and applications, pp 60–69. Ed. by Innis M. A, Gelfand D. H., Sninsky J. J & White T. J. Academic Press, INC, San Diego ET (1990).
K.E. Lipson and R. Baserga, Transcriptional activity of the human thymidine kinase gene determined by a method using the Polymerase chain reaction and a intron-specific probe. Proc. natl. Acad. Sci. USA 86:9774, (1989)
P.H. Ellims, M.B. Van der Weyden, G. Medley, Thymidine kinase isoenzymes in human malignant lymphoma. Cancer Res. 41:691 (1981)
Z-F. Chang and D-Y. Huang, The regulation of thymidine kinase in HL-60 human promyelo leukemia cells. J. Biol. Chem. 268:1266 (1993)
M.G. Kauffman and T.J. Kelly, Cell cycle regulation of thymidine kinase: residues near the carboxyl terminus are essential for the specific degradation of the enzyme at mitosis. Mol. Cell. Biol. 11:2538 (1991)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kristensen, T., Jensen, H.K., Munch-Petersen, B. (1995). Quantitation of TK1 mRNA in Patients with Chronic Lymphatic Leukemia. In: Sahota, A., Taylor, M.W. (eds) Purine and Pyrimidine Metabolism in Man VIII. Advances in Experimental Medicine and Biology, vol 370. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2584-4_6
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2584-4_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6105-3
Online ISBN: 978-1-4615-2584-4
eBook Packages: Springer Book Archive