Abstract
Purine metabolism is a key metabolic process after partial hepatectomy in the synthesis of DNA and RNA, and in the energy metabolism for hepatic regeneration1. However it is not evaluated in clinical situations due to the difficulty in measuring changes of purine levels in the liver itself. In order to get a basic understanding of purine metabolism in clinical hepatectomy cases, blood samples are used for analysis in this study. Also, the liver is the main nondietary source of purines in mammalian cells2 and changes in blood level may affect purine metabolism in various tissues.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
M. Itakura, T. Maeda, M. Tsuchiya, and K. Yamashita, Increased rate of de novo purine synthesis and its mechanism in regenerating liver, Am J Physiol. 251:G585 (1986).
A.W. Murry, The biological significance of purine salvage, Ann Rev Biochem 40: 811 (1971).
V. Stocchi, L. Cucchiarine, F. Canestrari, M. Piacentini, and G. Fornani, A very fast ion-pair reversed-phase HPLC method for the separation of the most significant nucleotides and their degradation products in human red blood cells, Anal Biochem. 167:181 (1987).
T. Yamamoto, Y. Moriwaki, S. Takahashi, T. Hada, and K. Higashino, Separation of hypoxanthine and xanthine from pyrazinamide and its metabolites in plasma and urine by high-performance liquid chromatography, J. Chromatogr. 382:270 (1986).
P. Kabasakalia, S. Kalliney, and A. Westcott, Enzymatic blood glucose determination by colorimetry of N, N-diethylailine-4-aminoantipyrine, Clin Chem. 20:606 (1974).
M. Usami, K. Furuchi, M. Ogino, J. Kotani, A. Iso, K. Oh, I. Yasuda, E. Sou, T. Kanamaru, H. Kasahara, Y. Saitoh, H. Yokoyama, and T. Yumisashi, Changes in purine and pyrimidine metabolism after partial hepatectomy in rats (in Japanese), Jap J Surg Metab Nutr. 27:35 (1993).
T. Kurokawa, T. Nonami, K. Kuroe, M. Satake, A. Harada, A. Nakano, and H. Takagi, Nucleotide metabolism in remnant rat liver after major hepatic resection (in Japanese), J Jap Surg Soc. 9:994 (1990).
S.H. Giler, O. Sperling, S. Brosh, I. Urca, and A. De Vries, Serum xanthine oxidase in jaundice, Clin Chim Acta. 63:37 (1975).
S. Ogoshi, M. Iwasa, T. Yonezawa, and T. Tamiya, Effect of nucleotide and nucleoside mixture on rats given total parenteral nutrition after 70% hepatectomy, J. Parenter Enterai Nutr. 9:339 (1985).
H. Ohyanagi, S. Nishimatu, Y. Kanbara, M. Usami, and Y. Saitoh, Effect of nucleosides and a nucleotide on DNA and RNA syntheses by the salvage and de novo pathway in primary monolayer cultures of hepatocytes and hepatoma cells, J Parenter Enterai Nutr. 13:51 (1989).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media New York
About this chapter
Cite this chapter
Usami, M. et al. (1995). The Effect of Partial Hepatectomy on Blood Purine Levels in Rats and Patients. In: Sahota, A., Taylor, M.W. (eds) Purine and Pyrimidine Metabolism in Man VIII. Advances in Experimental Medicine and Biology, vol 370. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2584-4_3
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2584-4_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6105-3
Online ISBN: 978-1-4615-2584-4
eBook Packages: Springer Book Archive