Abstract
The phospholipases A2 (PLA2s) comprise a growing enzyme superfamily1. PLA2s hydrolyze ester linked acyl groups at thesn-2position of membrane phospholipids. This activity liberates free fatty acid and lysophospholipid. In eukaryotic cells thesn-2position of membrane phospholipids is enriched with arachidonic acid and therefore the PLA2s are considered to be the first step in the regulated production of the eicosanoids. While eicosanoid production is of major importance in normal and pathological states the functions of individual PLA2s in normal and pathophysiological states is not known.
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References
E.A. Dennis, The growing phospholipase A2superfamily of signal transduction enzymes, Trends Biochem.Sci., 22: 1–2 (1997).
J.D. Clark, L.L. Lin, R.W. Kriz, C.S. Ramesha, L.A. Sultzman, A.Y. Lin, N. Milona, and J.L. Knopf, A novel arachidonic acid-selective cytosolic PLA2contains a Cat+- dependent translocation domain with homology to PKC and GAP, Cell, 65: 1043–51 (1991).
L.L. Lin, M. Wartmann, A.Y. Lin, J.L. Knopf, A. Seth, and R.J. Davis, cPLA2 is phosphorylated and activated by MAP kinase, Cell, 72: 269–78 (1993).
R.M. Kramer, E.F. Roberts, S.L. Um, A.G. Borsch-Haubold, S.P. Watson, M.J. Fisher, and J.A. Jakubowski, p38 mitogen-activated protein kinase phosphorylates cytosolic phospholipase A2 (cPLA2) in thrombin-stimulated platelets. Evidence that proline-directed phosphorylation is not required for mobilization of arachidonic acid by cPLA2, J Biol Chem, 271: 27723–9 (1996).
J.V. Bonventre, Z. Huang, M.R. Taheri, E. O’Leary, E. Li, M.A. Moskowitz, and A. Sapirstein, Reduced fertility and postischaemic brain injury in mice deficient in cytosolic phospholipase A2Nature, 390: 622–5 (1997).
N. Uozumi, K. Kume, T. Nagase, N. Nakatani, S. Ishii, F. Tashiro, Y. Komagata, K. Maki, K. Ikuta, Y. Ouchi, J. Miyazaki, and T. Shimizu, Role of cytosolic phospholipase A2in allergic response and parturition, Nature, 390: 618–22 (1997).
J. Pfeilschifter, Mesangial cells orchestrate inflammation in the renal glomerulus, NIPS, 9: 271–6 (1994).
S.C. Gad, B.J. Dunn, D.W. Dobbs, C. Reilly, and R.D. Walsh, Development and validation of an altemative dermal sensitization test: the mouse ear swelling test (MEST), Toxicol Appl Pharmacol, 84: 93–114 (1986).
R.P. Carlson, L. O’Neill-Davis, J. Chang, and A.J. Lewis, Modulation of mouse ear edema by cyclooxygenase and lipoxygenase inhibitors and other pharmacologic agents, Agents Actions, 17: 197–204 (1985).
X.S. Chen, J.R. Sheller, E.N. Johnson, and C.D. Funk, Role of leukotrienes revealed by targeted disruption of the 5- lipoxygenase gene, Nature, 372: 179–82 (1994).
R. Langenbach, S.G. Morham, H.F. Tiano, C.D. Loftin, B.I. Ghanayem, P.C. Chulada, J.F. Mahler, C.A. Lee, E.H. Goulding, K.D. Kluckman, and et al., Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration, Cell, 83: 483–92 (1995).
S.G. Morham, R. Langenbach, C.D. Loftin, H.F. Tiano, N. Vouloumanos, J.C. Jennette, J.F. Mahler, K.D. Kluckman, A. Ledford, C.A. Lee, and et al., Prostaglandin synthase 2 gene disruption causes severe renal pathology in the mouse, Cell, 83: 473–82 (1995).
H. Lim, B.C. Paria, S.K. Das, J.E. Dinchuk, R. Langenbach, J.M. Trzaskos, and S.K. Dey, Multiple female reproductive failures in cyclooxygenase 2-deficient mice, Cell, 91: 197–208 (1997).
C.R. Kennedy, Y. Zhang, S. Brandon, Y. Guan, K. Coffee, C.D. Funk, M.A. Magnuson, J.A. Oates, M.D. Breyer, and R.M. Breyer, Salt-sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor, Nat Med, 5: 217–20 (1999).
K. Abe, K. Kogure, H. Yamamoto, M. Imazawa, and K. Miyamoto, Mechanism of arachidonic acid liberation during ischemia in gerbil cerebral cortex, J Neurochem, 48: 503–9 (1987).
N.G. Bazan, Jr., Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brain, Biochim Biophys Acta, 218: 1–10 (1970).
P. Klivenyi, M.F. Beal, R.J. Ferrante, O.A. Andreassen, M. Wermer, M.R. Chin, and J.V. Bonventre, Mice deficient in group IV cytosolic phospholipase A2are resistant to MPTP neurotoxicity, J Neurochem, 71: 2634–7 (1998).
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Bonventre, J.V., Sapirstein, A. (2002). Group IV Cytosolic Phospholipase A2(Pla2) Function:Insights from the Knockout Mouse. In: Honn, K.V., Marnett, L.J., Nigam, S., Dennis, E., Serhan, C. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 5. Advances in Experimental Medicine and Biology, vol 507. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0193-0_5
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DOI: https://doi.org/10.1007/978-1-4615-0193-0_5
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