Abstract
Tyrophagus putrescentiae and Dermatophagoides pteronyssinus are causative factors for the development of airway hypersensitivity. The main objective in this study was to identify the cross-reactive allergens between T. putrescentiae and D. pteronyssinus and investigate their sensitization in patients with allergic rhinitis. The prevalence of sensitization to mites was determined by skin prick tests and histamine release assays. Both immunoblot and ELISA inhibition assays were performed by using the recombinant allergens of T. putrescentiae and D. pteronyssinus. The cross-reactive allergens were identified by using IgE-binding inhibition analysis. The correlations of specific IgE between T. putrescentiae and D. pteronyssinus to group 2 and group 3 mite allergens were compared. A total of 117 allergic rhinitis patients, aged between 16 and 40 years old were recruited to be included in this study. The results showed that 70% (82/117) of allergic rhinitis subjects had skin test positive reactions to D. pteronyssinus or T. putrescentiae. Among these mite-sensitive subjects, there were 81 subjects (81/82) sensitive to D. pteronyssinus and 34 subjects (34/82) sensitive to T. putrescentiae. Among the T. putrescentiae hypersensitive subjects, 97% (33/34) were also sensitized to D. pteronyssinus. In the IgE-binding inhibition analysis, 59% (13/22) subjects had IgE-binding activity of T. putrescentiae that was completely absorbed by D. pteronyssinus, especially components with MW at 16 kDa. In ELISA inhibition testing, 69% of IgE-binding was inhibited by rTyr p 2, and 45% inhibited by rTyr p 3. The titers of IgE antibodies to rTyr p 2 and rDer p 2 were well correlated, but not rTyr p 3 and rDer p 3. In conclusion, most T. putrescentiae sensitized subjects were also sensitized to D. pteronyssinus in young adult allergic rhinitis patients. The complete absorption of IgE binding activity by D. pteronyssinus indicates that T. putrescentiae hypersensitivity might be due to the cross-reactivity, not dual-sensitization of D. pteronyssinus and T. putrescentiae. The IgE-binding titers of group 2 allergens were well correlated and the binding activity of Tyr p 2 could be absorbed by Der p 2, suggesting that group 2 allergens are the major cross-reactive allergen of D. pteronyssinus and T. putrescentiae.
Similar content being viewed by others
References
Simplicio EC, Silva DA, Braga IA, Sopelete MC, Sung SJ, Taketomi EA. Mite and pet allergen exposure in hotels in Uberlandia, Midwestern Brazil. Indoor Air. 2007;17:278–83.
Cates EC, Fattouh R, Johnson JR, Llop-Guevara A, Jordana M. Modeling responses to respiratory house dust mite exposure. Contrib Microbiol. 2007;14:42–67.
Murray CS, Woodcock A, Custovic A. The role of indoor allergen exposure in the development of sensitization and asthma. Curr Opin Allergy Clin Immunol. 2001;1:407–12.
Diette GB, McCormack MC, Hansel NN, Breysse PN, Matsui E. Environmental issues in managing asthma. Respir Care. 2008;53:602–15.
Bush RK. Indoor allergens, environmental avoidance, and allergic respiratory disease. Allergy Asthma Proc. 2008;29:575–9.
Arlian LG, Morgan MS. Biology, ecology, and prevalence of dust mites. Immunol Allergy Clin North Am. 2003;23:443–68.
Fernandez-Caldas E, Iraola V, Carnes J. Molecular and biochemical properties of storage mites (except Blomia species). Protein Pept Let. 2007;14:954–9.
Thomas WR, Smith WA, Hales B. The allergenic specificities of the house dust mite. Chang Gung Med J. 2004;27:563–9.
Yi FC, Shek LP, Cheong N, Chua KY, Lee B. Molecular cloning of Blomia tropicalis allergens—a major source of dust mite allergens in the tropics and subtropics. Inflamm Allergy Drug Targets. 2006;5:261–6.
Kondreddi PK, Elder BL, Morgan MS, Vyszenski-Moher DL, Arlian L. Importance of sensitization to Tyrophagus putrescentiae in the United States. Ann Allergy Asthma Immunol. 2006;96:124.
Vidal C, Boquete O, Gude F, Rey J, Meijide LM, Fernández-Merino MC, et al. High prevalence of storage mite sensitization in a general adult population. Allergy. 2004;59:401–5.
Park JW, Ko SH, Yong TS, Ree HI, Jeoung BJ, Hong CS. Cross-reactivity of Tyrophagus putrescentiae with Dermatophagoides farinae and Dermatophagoides pteronyssinus in urban areas. Ann Allergy Asthma Immunol. 1999;83:533–9.
Liao EC, Hsu EL, Tsai JJ, Ho CM. Immunologic characterization and allergenicity of recombinant Tyr p 3 allergen from the storage mite Tyrophagus putrescentiae. Int Arch Allergy Immunol. 2009;150:15–24.
Tsai JJ, Ho CY, Wang SR. Relationship between nasal resistance and airway hyperreactivity following nasal provocation with Dermatophagoides pteronyssinus in allergic rhinitis. Int Arch Allergy Immunol. 1995;106:286–90.
Kam KL, Hsieh KH. Comparison of three in vitro assays for serum IgE with skin testing in asthmatic children. Ann Allergy. 1994;73:329–36.
Thomas WR, Smith WA, Hales BJ, Mills KL, O'Brien RM. Characterization and immunobiology of house dust mite allergens. Int Arch Allergy Immunol. 2002;129:1–18.
Henszel L, Kuzna-Grygiel W. House dust mites in the etiology of allergic diseases. Ann Acad Med Stetin. 2006;52:123–7.
Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR. Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. Int Arch Allergy Immunol. 1998;115:144–9.
Arlian LG, Geis DP, Vyszenski-Moher DL, Bernstein IL, Gallagher JS. Antigenic and allergenic properties of the storage mite Tyrophagus putrescentiae. Int Arch Allergy Immunol. 1984;74:166–71.
Eriksson TL, Johansson E, Whitley P, Schmidt M, Elsayed S, van Hage-Hamsten M. Cloning and characterisation of a group II allergen from the dust mite Tyrophagus putrescentiae. Eur J Biochem. 1998;251:443–7.
Liao EC, Ho CM, Tsai JJ. The prevalence of Tyrophagus putrescentiae hypersensitivity in subjects over 70 years of age in a veterans' nursing home in Taiwan. Int Arch Allergy Immunol. 2010;152:368–77.
Smith WA, Chua KY, Kuo MC, Rogers BL, Thomas WR. Cloning and sequencing of the Dermatophagoides pteronyssinus group III allergen, Der p III. Clin Exp Allergy. 1994;24:220–8.
Tsai JJ, Shen HD, Chua KY. Purification of group 2 Dermatophagoides pteronyssinus allergen and prevalence of its specific IgE in asthmatics. Int Arch Allergy Immunol. 2000;121:205–10.
Cui Y, Zhou P, Peng J, Peng M, Zhou Y, Lin Y, et al. Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli. Braz J Med Biol Res. 2008;41:380–8.
Wu CC, Liao EC, Lee MF, Tsai JJ. Augmentation of regulatory T cells in allergic individuals by recombinant Der f 2 peptide with fungal immunomodulatory peptide fve. Ann Allergy Asthma Immunol. 2009;102:216–22.
Yoshimura T, Kaneuchi T, Miura T, Kimura M. Kinetic analysis of the fluorescence reaction of histamine with orthophthalaldehyde. Anal Biochem. 1987;164:132–7.
Johansson E, Johansson SG, Van Hage-Hamsten M. Allergenic characterization of Acarus siro and Tyrophagus putrescentiae and their cross-reactivity with Lepidoglyphus destructor and Dermatophagoides pteronyssinus. Clin Exp Allergy. 1994;24:743–51.
Acknowledgements
This study was supported by grant (TCVGH-977310C) from Taichung Veterans General Hospital and grant (NSC97-2320-B010-008-MY3) from the National Science Council of Taiwan. The authors sincerely appreciate the assistance of the TCVGHAGE research group and Biostatistics Task Force of Taichung Veterans General Hospital, Taichung, Taiwan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liao, EC., Ho, CM., Lin, MY. et al. Dermatophagoides pteronyssinus and Tyrophagus putrescentiae Allergy in Allergic Rhinitis Caused by Cross-reactivity Not Dual-Sensitization. J Clin Immunol 30, 830–839 (2010). https://doi.org/10.1007/s10875-010-9446-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10875-010-9446-x