J. Naric was responsible for data compilation, data analysis and writing of the manuscript. J. Rissland was responsible for data interpretation of virological results and drafting of the manuscript. A. Simon was responsible for data interpretation and drafting of the manuscript. M. Poryo was responsible for data analysis and writing of the manuscript. L. Gortner was responsible for data analysis and writing of the manuscript. S. Meyer was chief investigator. He was responsible for study/audit design, data interpretation and writing of the original manuscript. S. Meyer and J. Naric contributed equally to this work.
The aim of this study was to assess multiplex PCR analysis in detecting causative viruses in children with febrile seizures.
The study was a retrospective analysis comparing data from a pre-multiplex era (2009) with a period after the introduction of routine respiratory multiplex analysis (2010–2013) in children with febrile seizures.
We included 200 children with febrile seizures (mean age: 29.5 ± 1.4.months; 104 male) in the study. In 2009, in 10 out of 49 (20 %) children, microbiology testing (bacterial/fungal) was positive compared with a rate of 74 out of 151 (49 %) children during 2010–2013 (p < 0.01). The rate of positive virological studies increased from 10 (20 %) in 2009 to 73 (48.3 %) in the period 2010–2013 (p < 0.01). Multiplex PCR analysis confirmed viral infections in 52 of 73 cases (71.2 %).
Routine multiplex PCR analysis fosters the detection of respiratory viruses in children with febrile seizure. The precise role of multiplex analysis in the management of these children awaits further clarification.