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Erschienen in: Wiener Medizinische Wochenschrift 11-12/2017

20.06.2016 | original article

Role of multiplex PCR analysis in children with febrile seizures

verfasst von: M.S. Jelena Naric, M.D. Jürgen Rissland, M.D. Prof. Arne Simon, M.D. Martin Poryo, M.D. Prof. Ludwig Gortner, M.D. Associate Prof. Dr. Sascha Meyer

Erschienen in: Wiener Medizinische Wochenschrift | Ausgabe 11-12/2017

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Summary

Background

The aim of this study was to assess multiplex PCR analysis in detecting causative viruses in children with febrile seizures.

Methods

The study was a retrospective analysis comparing data from a pre-multiplex era (2009) with a period after the introduction of routine respiratory multiplex analysis (2010–2013) in children with febrile seizures.

Results

We included 200 children with febrile seizures (mean age: 29.5 ± 1.4.months; 104 male) in the study. In 2009, in 10 out of 49 (20 %) children, microbiology testing (bacterial/fungal) was positive compared with a rate of 74 out of 151 (49 %) children during 2010–2013 (p < 0.01). The rate of positive virological studies increased from 10 (20 %) in 2009 to 73 (48.3 %) in the period 2010–2013 (p < 0.01). Multiplex PCR analysis confirmed viral infections in 52 of 73 cases (71.2 %).

Conclusion

Routine multiplex PCR analysis fosters the detection of respiratory viruses in children with febrile seizure. The precise role of multiplex analysis in the management of these children awaits further clarification.
Literatur
2.
Zurück zum Zitat Subcommittee on Febrile Seizures. Febrile seizures: Guideline for a neurodiagnostic evaluation of the child with simple febrile seizure. Pediatrics. 2011;127:389. CrossRef Subcommittee on Febrile Seizures. Febrile seizures: Guideline for a neurodiagnostic evaluation of the child with simple febrile seizure. Pediatrics. 2011;127:389. CrossRef
3.
Zurück zum Zitat Mastrangelo M, Midulla F, Moretti C. Actual insights into the clinical management of febrile seizures. Eur J Pediatr. 2014;173:977–82. PubMed Mastrangelo M, Midulla F, Moretti C. Actual insights into the clinical management of febrile seizures. Eur J Pediatr. 2014;173:977–82. PubMed
4.
Zurück zum Zitat Shah PB, James S, Elayaraja S. EEG for children with complex febrile seizures. Cochrane Database Syst Rev. 2014;1:CD009196 doi:10.1002/14651858.CD009196.pub2. Shah PB, James S, Elayaraja S. EEG for children with complex febrile seizures. Cochrane Database Syst Rev. 2014;1:CD009196 doi:10.1002/14651858.CD009196.pub2.
5.
Zurück zum Zitat Pavlidou E, Hagel C, Panteliadis C. Febrile seizures: recent developments and unanswered questions. Childs Nerv Syst. 2013;29:2011–7. CrossRefPubMed Pavlidou E, Hagel C, Panteliadis C. Febrile seizures: recent developments and unanswered questions. Childs Nerv Syst. 2013;29:2011–7. CrossRefPubMed
6.
Zurück zum Zitat Berg AT, Jallon P, Preux PM. The epidemiology of seizure disorders in infancy and childhood: definitions and classifications. Handb Clin Neurol. 2013;111:391–8. CrossRefPubMed Berg AT, Jallon P, Preux PM. The epidemiology of seizure disorders in infancy and childhood: definitions and classifications. Handb Clin Neurol. 2013;111:391–8. CrossRefPubMed
7.
Zurück zum Zitat Patel AD, Vidaurre J. Complex febrile seizures: a practical guide to evaluation and treatment. J Child Neurol. 2013;28:762–7. CrossRefPubMed Patel AD, Vidaurre J. Complex febrile seizures: a practical guide to evaluation and treatment. J Child Neurol. 2013;28:762–7. CrossRefPubMed
8.
Zurück zum Zitat Sanghavi SK, Bullotta A, Husain S, Rinaldo CR. Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections. J Med Vir. 2012;84:162–9. CrossRef Sanghavi SK, Bullotta A, Husain S, Rinaldo CR. Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections. J Med Vir. 2012;84:162–9. CrossRef
9.
Zurück zum Zitat Schreckenberger PC, McAdam AJ. Point-Counterpoint: large multiplex PCR panels should be first-line tests for detection of respiratory and intestinal pathogens. J Clin Microbiol. 2015;53:3110–5. CrossRefPubMedPubMedCentral Schreckenberger PC, McAdam AJ. Point-Counterpoint: large multiplex PCR panels should be first-line tests for detection of respiratory and intestinal pathogens. J Clin Microbiol. 2015;53:3110–5. CrossRefPubMedPubMedCentral
10.
Zurück zum Zitat Krause JC, Panning M, Hengel H, Henneke P. The role of multiplex PCR in respiratory tract infections in children. Dtsch Arztebl Int. 2014;111:639–45. PubMedPubMedCentral Krause JC, Panning M, Hengel H, Henneke P. The role of multiplex PCR in respiratory tract infections in children. Dtsch Arztebl Int. 2014;111:639–45. PubMedPubMedCentral
11.
Zurück zum Zitat Sakthivel SK, Whitaker B, Lu X, Oliveira DBL, Stockman LJ, Kamili S, Oberste MS, Erdmann DD. Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses. J Virol Methods. 2012;185(2):259–66. CrossRefPubMed Sakthivel SK, Whitaker B, Lu X, Oliveira DBL, Stockman LJ, Kamili S, Oberste MS, Erdmann DD. Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses. J Virol Methods. 2012;185(2):259–66. CrossRefPubMed
12.
Zurück zum Zitat Anderson TP, Werno AM, Barrat K, Mahagamasekera P, Murdoch DR, Jennings LC. Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens. J Virol Methods. 2013;191:118–21. CrossRefPubMed Anderson TP, Werno AM, Barrat K, Mahagamasekera P, Murdoch DR, Jennings LC. Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens. J Virol Methods. 2013;191:118–21. CrossRefPubMed
13.
Zurück zum Zitat Bierbaum S, Forster J, Berner R, Rücker G, Rhode G, Neumann-Haefelin D, Panning M. (CAPNETZ study group). Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10. Arch Virol. 2014;159:669–76. CrossRefPubMed Bierbaum S, Forster J, Berner R, Rücker G, Rhode G, Neumann-Haefelin D, Panning M. (CAPNETZ study group). Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10. Arch Virol. 2014;159:669–76. CrossRefPubMed
14.
Zurück zum Zitat García-Arroyo L, Prim N, Martí N, Roig MC, Navarro F, Rabella N. Benefits and drawbacks of molecular techniques for diagnosis of viral respiratory infections. Experience with two multiplex PCR assays. J Med Virol. 2016;88:45–50. CrossRefPubMed García-Arroyo L, Prim N, Martí N, Roig MC, Navarro F, Rabella N. Benefits and drawbacks of molecular techniques for diagnosis of viral respiratory infections. Experience with two multiplex PCR assays. J Med Virol. 2016;88:45–50. CrossRefPubMed
15.
Zurück zum Zitat Oluwabusi T, Sood SK. Update on the management of simple febrile seizures: emphasis on minimal intervention. Curr Opin Pediatr. 2012;24:259–65. CrossRefPubMed Oluwabusi T, Sood SK. Update on the management of simple febrile seizures: emphasis on minimal intervention. Curr Opin Pediatr. 2012;24:259–65. CrossRefPubMed
16.
Zurück zum Zitat Wishaupt JO, Russcher A, Smeets LC, Versteegh FGA, Hartwig NG. Clinical impact of RT-PCR for pediatric acute respiratory infections: a controlled clinical trial. Pediatrics. 2011;128:e1113–1120. CrossRefPubMed Wishaupt JO, Russcher A, Smeets LC, Versteegh FGA, Hartwig NG. Clinical impact of RT-PCR for pediatric acute respiratory infections: a controlled clinical trial. Pediatrics. 2011;128:e1113–1120. CrossRefPubMed
17.
Zurück zum Zitat Hyun DY, Hersh AL, Namtu K, Palazzi DL, Maples HD, Newland JG, Saiman L. Antimicrobial stewardship in pediatrics: how every pediatrician can be a steward. JAMA Pediatr. 2013;167:859–66. CrossRefPubMed Hyun DY, Hersh AL, Namtu K, Palazzi DL, Maples HD, Newland JG, Saiman L. Antimicrobial stewardship in pediatrics: how every pediatrician can be a steward. JAMA Pediatr. 2013;167:859–66. CrossRefPubMed
Metadaten
Titel
Role of multiplex PCR analysis in children with febrile seizures
verfasst von
M.S. Jelena Naric
M.D. Jürgen Rissland
M.D. Prof. Arne Simon
M.D. Martin Poryo
M.D. Prof. Ludwig Gortner
M.D. Associate Prof. Dr. Sascha Meyer
Publikationsdatum
20.06.2016
Verlag
Springer Vienna
Erschienen in
Wiener Medizinische Wochenschrift / Ausgabe 11-12/2017
Print ISSN: 0043-5341
Elektronische ISSN: 1563-258X
DOI
https://doi.org/10.1007/s10354-016-0462-1

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