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14.11.2019 | review Open Access

Role of CAR-T cell therapy in B-cell acute lymphoblastic leukemia

Zeitschrift:
memo - Magazine of European Medical Oncology
Autor:
Univ. Prof. Dr. Hildegard T. Greinix
Wichtige Hinweise

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Abbreviations
A
Adults
ALL
Acute lymphoblastic leukemia
ASTCT
American Society of Transplantation and Cellular Therapy
BM
Bone marrow
BU
Busulfan
C
Children
CAR
Chimeric antigen receptor
CCR
Continuous complete remission
CD19
Cluster of differentiation 19
CR
Complete remission
CRh
Complete remission with partial hematologic recovery
CRi
Complete remission with incomplete regeneration
CRS
Cytokine release syndrome
CSF
Cerebrospinal fluid
CY
Cyclophosphamide
EFS
Event-free survival
EMA
European Medicines Agency
FDA
Food and Drug Administration
Flu
Fludarabine
GvHD
Graft-versus-host disease
HSCT
Hematopoietic stem cell transplantation
ICANS
Immune-cell associated neurotoxicity syndrome
ICE
Immune effector cell-associated encephalopathy
IFN
Interferon
IL
Interleukin
InO
Inotuzumab ozogamicin
LV
Lentivirus
MHC
Major histocompatibility complex
MRD
Minimal residual disease
NC
No change
NR
No response
ORR
Overall response rate
OS
Overall survival
PB
Peripheral blood
PCR
Polymerase chain reaction
PD
Progressive disease
PR
Partial remission
RV
Retrovirus
scFv
Single-chain variable antibody fragment
SD
Stable disease
SOC
Standard of care
TALEN
Transcription activator-like effector nuclease
TCR
T cell receptor
TNF
Tumor necrosis factor
TRUCK
T cells redirected for universal cytokine-mediated killing
TSLPR
Thymic stromal lymphopoietin receptor
UCART19
Universal CD19-CAR-T cell
Take home message
CD19-CAR‑T cells are of enormous benefit for patients with relapsed/refractory ALL.
Further improvements in CAR‑T cell constructs may allow longer term remissions without additional HSCT.

Introduction

First-line chemotherapy for patients with B‑cell acute lymphoblastic leukemia (B-ALL) younger than 65 years is intensive chemotherapy, e.g., Hoelzer protocol or hyper-CVAD (1). Allogeneic hematopoietic stem cell transplantation (HSCT) is indicated in B‑ALL for patients with a second complete remission (CR), after failure of first-line chemotherapy or upfront in first CR in B‑ALL with unfavorable prognostic factors, including complex karyotype and positive minimal residual disease (MRD) [1]. Relapsed or refractory B‑ALL is associated with a dismal prognosis with a cure rate of less than 10% [1]. CR rates with standard chemotherapy regimens are 30–40% in first relapse and 20–25% in second relapse [1]. Only 10–30% of adult patients with relapsed B‑ALL proceed to HSCT, which is the only curative therapy in this situation. Novel therapeutic options including blinatumomab and inotuzumab ozogamicin (InO) have resulted in higher response rates and longer survival (OS) than conventional chemotherapy [1]. Response to these agents enables patients to undergo HSCT as has been recently reported by Marks and colleagues where 101 of 236 patients (43%) with relapsed/refractory B‑ALL given InO, an anti-CD22 antibody conjugated to calicheamicin, proceeded to HSCT [2]. Of note, for patients with no previous HSCT who went directly to transplant after achieving remission to InO, the 2‑year survival probability was 46%. In a prospective randomized phase III study more patients with relapsed/refractory B‑ALL treated with blinatumomab, a bispecific T cell engager targeted to CD19 and CD3, compared to standard-of-care chemotherapy (SOC) achieved CR, CR with partial (CRh), or incomplete (CRi) hematologic recovery and MRD-negativity [3]. Blinatumomab prolonged OS with a median OS of 7.7 months versus 4.0 months for SOC, respectively. Patients achieving CR, CRh, or CRi after therapy with blinatumomab reportedly achieved durable responses and promising OS rates with or without subsequent HSCT [4]. Due to study limitations it is currently unclear whether patients responding to blinatumomab and obtaining MRD-negativity should undergo HSCT.
A novel cellular immunotherapeutic approach involves the genetic modification of T cells to express a chimeric antigen receptor (CAR) thereby redirecting their specificity through a mechanism independent of major histocompatibility complex (MHC) to target specific tumor antigens [5]. CARs are engineered fusion proteins combining an extracellular epitope-specific binding domain (most commonly an antibody-derived single-chain variable fragment, scFv), a hinge and transmembrane domain and signaling domains of the T cell receptor (TCR) (mostly consisting of the CD3ζ chain). Current second-generation CAR‑T constructs are combined with additional costimulatory domains such as CD28, 4‑1BB, and OX40. This enables a strong antigen-specific T cell activation without the need of TCR-MHC interactions. By their integrative capacity into the host genome, expression of CAR transgenic construct can persist independent of cell division.

Clinical results with CAR-T cells in relapsed and refractory B-ALL

Most of the targeting immunotherapies involving CAR‑T cells in B‑ALL are against the B cell surface protein CD19 and CAR‑T cell products may vary depending on the institutional design, doses, T cell activation and transduction methods. Table 1 summarizes the results of CAR‑T cell studies in patients with relapsed and refractory B‑ALL.
Table 1
Clinical results of CAR‑T cell therapy in relapsed and refractory ALL
Author
No. patients
Gene transfer
Co-stim. domain
Lymphodepl. chemo
CAR‑T cell doses
Outcome
Brentjens (2011) [27]
1
Gamma RV
CD28
CY
1.4 × 108
N/A
Brentjens (2013) [27]
5 A
Gamma RV
CD28
CY
1.4–3.2 × 108
5 CR
Cruz (2013) [27]
8
RV
CD28
1.9 × 107–1.13 × 108
1 CR
2 CCR
1 PR
3 PD
1 SD
Grupp (2013) [28]
2 C
LV
CD28
1.4 × 106–1.2 × 107/kg
2 CR
Davila (2014) [27]
16 A
Gamma RV
CD28
CY
3 × 106/kg
15 CR
Maude (2014) [8]
30
(25C/5A)
LV
4‑1BB
CY/Flu or CY/VP or other
0.76–17.36 × 106/kg
27 CR
3 NR
(19 CCR)
Dai (2015) [27]
9
LV
4‑1BB
C‑MOAD
3 × 106–1 × 107/kg
6 CR
3 PR
Lee (2015) [17]
21 C+A
Gamma RV
CD28
CY/Flu
1 × 106 vs 3 × 106/kg
14 CR
4 PD
3 SD
Park (2015) [29]
33 A
N/A
N/A
N/A
N/A
91% CR
Gardner (2016) [27]
9
N/A
N/A
Chemo
2 × 106–1 × 107/kg
7 CR
2 Relapse
Turtle (2016) [10]
30 A
LV
CD28
CY/Flu
2 × 105–2 × 107/kg
29 CR (27 MRD)
Zhu (2016) [27]
2
LV
4‑1BB
CY/Flu
1 × 106/kg
2 CR
Callahan (2017) [30]
59
LV + RV
N/A
Chemo
N/A
55 CR
20 Relapse
Chen (2017) [27]
6
LV
CD28
CY/Flu
3.8 × 107–4.1 × 108/kg
5 CR (MRD)
1 NR
4 Relapse
Gardner (2017) [11]
45 C+A
LV
4‑1BB
CY, CY/Flu
5 × 105–1 × 107/kg
40 CR (MRD)
3 NC
18 Relapse
Pan (2017) [27]
51
LV
4‑1BB
CY/Flu
5 × 103–1.4 × 107/kg
45 CR (9 MRD)
3 NR
3 died
2 Relapse
Wang (2017) [27]
6
N/A
N/A
CY/BU/Flu
1.2–8.5 × 106/kg
3 CR
1 died
2 NR
2 Relapse
Fry (2018) [27]
21
LV
4‑1BB
CY/Flu
3 × 105–3 × 106/kg
12 CR (9 MRD)
8 relapse
Maude (2018) [6]
75 C+A
LV
4‑1BB
CY/Flu
0.2–5.4 × 106/kg
45 CR+16 CRi (61 MRD)
22 Relapse
Park (2018) [9]
53 A
Gamma RV
CD28
CY/Flu
N/A
44 CR (32 MRD)
25 Relapse
Park (2018) [27]
2
LV
4‑1BB
CY/Flu
4.6 × 106/kg
1 CR
Wei (2018) [31]
22
LV
4‑1BB
CY/Flu
3–10 × 107/kg
20 CR
2 SD
1 died
8 Relapse
Weng (2018) [32]
5
LV
CD28
CY/Flu
5 × 104–1 × 106/kg
3 CR
2 Relapse
N/A Not applicable, No. number, co-stim co-stimulatory, lymphodepl lymphodepleting, chemo chemotherapy, A adults, C children, RV retrovirus, LV lentivirus, CY cyclophosphamide, VP etoposide, Flu Fludarabine, BU busulfan, CR complete remission, CCR continuous complete remission, PR partial remission, PD progressive disease, SD stable disease, NR no response, NC no change, MRD minimal residual disease negative
In a phase 2 multicenter study, 107 patients with relapsed or refractory B‑ALL were screened, 92 were enrolled and 75 (70%) received a single infusion of tisagenlecleucel, a CD19-CAR‑T cell product [6]. Among evaluable patients, the overall response rate (ORR) at 3 months was 81% with all responding patients negative for MRD as assessed by flow cytometry. In an intent-to-treat analysis of all enrolled 92 patients including subjects who discontinued study participation before tisagenlecleucel infusion, the ORR was 66%. The rates of event-free survival (EFS) and OS were 73 and 90% at 6 months and 50 and 76% at 12 months, respectively. The median duration of persistence of tisagenlecleucel in blood was 168 (range, 20–617) days and the median duration of remission was not reached at the time of reporting. Based on these results tisagenlecleucel was recently approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for refractory or relapsed B‑cell precursor ALL in children and young adults <25 years old.
Using quantitative polymerase chain reaction (PCR) to quantify levels of tisagenlecleucel, responding patients (n = 62/79) of two studies in pediatric B‑ALL had an approximately 2‑fold higher tisagenlecleucel expansion in peripheral blood (PB) than nonresponders with persistence measurable beyond 2 years in responding patients [7]. Clinical responses were observed across the entire dose range evaluated with no relationship between cell dose and safety. Tisagenlecleucel persistence was significantly associated with durable remission [8].
Park and colleagues reported long-term follow-up results of a single-center phase I clinical trial with CD19-CAR‑T cells in 53 adult patients with relapsed or refractory B‑ALL [9]. CR was observed in 83% of patients including 63% free of MRD. At a median follow-up of 29 (range, 1–65) months, median EFS was 6.1 months and median OS was 12.9 months, respectively. Patients with a low disease burden defined as less than 5% bone marrow (BM) blasts before therapy had longer remission duration and survival with a median EFS of 10.6 months and a median OS of 20.1 months. All 9 patients with detectable residual disease relapsed, while among 32 patients without MRD after CAR‑T cell therapy, 16 patients (50%) relapsed.
Among 30 adult patients with B‑ALL treated with a defined composition of CD4+ and CD8+ CAR‑T cells, 29 patients achieved a CR including 27 MRD CR [10]. Gardner and colleagues administered a CAR‑T cell product of defined CD4/CD8 composition to 45 children and young adults with relapsed or refractory B‑ALL resulting in MRD CR in 40 (89%) patients [11]. The estimated 12-month EFS was 50.8% and the estimated 12-month OS 69.5% with a median follow-up of 9.6 months. Eighteen of the 40 patients with MRD CR experienced relapse including 7 with loss of cell-surface detection of CD19. Median time from CAR‑T cell infusion to relapse was 5.98 months.

Side-effects of CAR-T cell therapy

The potent systemic immune activation responsible for the success of CAR‑T cells also drives novel life-threatening toxicities associated with immune effector cells including cytokine release syndrome (CRS) and immune-cell associated neurotoxicity syndrome (ICANS) [12].
CRS has been defined as a disorder characterized by fever, tachypnea, headache, tachycardia, hypotension, rash, arthralgia, myalgia, and/or hypoxia by the release of proinflammatory cytokines including interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), interleukin‑6 (IL-6), and interleukin-10 (IL-10) [13]. The first symptoms occur within hours up to 14 days after CAR‑T cell therapy. Reported incidence rates of CRS vary from 40–100% with severe forms in 20–30% of patients afflicted. Disease burden has most consistently been associated with CRS severity after CAR‑T cell therapy [13]. Whether strength of T cell activation and degree of T cell expansion as well as intensity of lymphodepletion prior to CAR‑T cell infusion have an impact on development of CRS remains controversial. Since pre-existing inflammation and endothelial activation seem to be associated with severity of CRS, CAR‑T cells should not be administered to patients with pre-existing inflammation and infection [14]. Recently, the American Society of Transplantation and Cellular Therapy (ASTCT) consensus grading has been published distinguishing CRS grades according to practitioner intervention [12].
The reported incidences of grade 3 to 4 ICANS after CAR‑T cell therapy in patients with B‑ALL are between 13% [6] and 42% [15] and could be influenced by the CAR design used. The earliest symptoms of ICANS are tremor, dysgraphia, mild difficulty with expressive speech, impaired attention, apraxia, and mild lethargy [12]. Expressive aphasia, starting as impaired naming of objects, hesitant speech, and verbal perseveration, may progress to global aphasia. Within hours or days severe neurotoxicity including seizures, diffuse cerebral edema, stupor, or even coma may occur. The ASTCT consensus recently recommended use of the Immune Effector Cell-Associated Encephalopathy (ICE) score for objective grading of ICANS [12].
The pathophysiology of ICANS is poorly understood. IL‑1 triggered activation of by-standing monocytes then producing IL‑6 und leading to systemic inflammation could be an important mechanism and blockade of IL‑1 with anakinra ameliorated ICANS’ symptoms in a xenograft mouse model [16]. Santomasso and colleagues reported a significant association of severe neurotoxicity with high pretreatment disease burden, higher peak CAR‑T cell expansion, and early and higher elevations of proinflammatory cytokines in PB [15]. Patients with severe neurotoxicity had evidence of blood–cerebrospinal fluid (CSF) barrier disruption and enrichment of proinflammatory cytokines in the CSF with disproportionately high levels of IL‑6, IL‑8, MCP1, and IP10 [15].
Management of ICANS is based on corticosteroids in escalating doses depending on the severity of disease and supportive care measures [13]. Patients unresponsive to steroids could benefit from siltuximab (anti-IL‑6 chimeric monoclonal antibody), anakinra (recombinant human IL‑1 receptor antagonist) or inhibition of GM-CSF using lenzilumab [13].

Novel developments for improving CAR-T cell therapy

Despite the high initial response rate with CD19-CAR‑T cells in B‑ALL, relapses occur in a significant fraction of patients [6, 8, 10, 18]. Relapse with CD19+ leukemia cells can be the result of short in vivo persistence of CAR‑T cells, either from intrinsic deficiencies of the T cell product or an immune response to the CAR scFv [10]. Strategies to improve CAR‑T cell efficacy focus on improved persistence of CAR‑T cells by combining CD4+ CAR‑T cells and CD8+ CAR‑T cells [10, 11], enriching for central memory T cells, naive and stem memory T cells or reducing the immunogenicity of the CAR construct applying humanized CARs [18]. Efficacy of CAR‑T cell immunotherapy can be improved by use of bispecific CARs targeting CD19 and CD22 [19] or CARs with multispecificity to overcome immune escape [20]. The thymic stromal lymphopoietin receptor (TSLPR) that is overexpressed on some B‑ALL cells could be a new target for developing novel CAR constructs. Furthermore, new designs of CAR‑T cells are being explored including universal CARs that can recognize multiple targets without the need to re-engineer T cells, T cells redirected for universal cytokine-mediated killing (TRUCKs), CARs and armored CAR‑T cells modified to express cytokines, ligands, or single-chain variable fragments to elicit an enhanced antitumor immune response through turning a suppressive tumor microenvironment into a proinflammatory one. In addition, combining CAR‑T cells with checkpoint blockade therapy may reinvigorate endogenous tumor-reactive T cells that have been suppressed by the tumor microenvironment [21].
Recently, Ruella and colleagues reported a unique case of a pediatric patient with B‑ALL who relapsed with CD19-negative disease after CAR‑T cell therapy due to introduction of anti-CD19 CAR into a single leukemic B cell blast clone during CAR‑T manufacturing [22]. This case demonstrates that more stringent CAR‑T cell manufacturing methods that remove all tumor cells from the genetically engineered product are necessary, especially for protocols that involve lentiviral vectors capable of transducing nondividing cells.

Allogeneic CAR-T cells

Currently, the approved CAR‑T cell products are from autologous T cells generated on a custom-made basis by a costly and lengthy production process with an inherent risk of production failure. For large scale clinical applications universal CAR‑T cells that can serve as “off-the-shelf” ready-to-use therapeutic agents would be of advantage. Recently, gene editing technologies including TALEN (transcription activator-like effector nuclease) and CRISPR/Cas9 are being used to improve CAR‑T cells and to generate universal third party CAR‑T cells [23]. These represent a totally new generation of CAR‑T cells capable of targeting multiple antigens and/or being delivered to multiple recipients without re-editing of T cells.
Qasim and colleagues used TALENs and a lentiviral vector to generate a universal CD19-CAR‑T cell (UCART19) with disrupted expression of both CD52 and the αβ TCR for treatment of 2 pediatric patients with relapsed B‑ALL [24]. Both patients achieved a CR within 28 days of CAR‑T cell therapy and then underwent successfully a second allogeneic HSCT with minimal evidence of graft-versus-host disease (GvHD). Currently, international multicenter off-the-shelf universal UCART19 trials are ongoing for CD19+ ALL.

CAR-T cells in the context of allogeneic HSCT

The available data demonstrate that CAR‑T cells are highly active in children and adults with refractory or relapsed B‑ALL resulting in a high complete remission rate. In a subset of patients MRD-negative remissions persist with no further treatment. However, follow-up times are short and relapse after CAR‑T cell therapy has remained a challenging problem. CAR‑T cell therapy could be applied to obtain remissions for patients in first relapse who are refractory or not eligible for other therapies to achieve remission prior to allogeneic HSCT. Shalabi and colleagues reported on 52 children and young adults given CD19 and 33 patients given CD22-CAR‑T cells for treatment of relapsed/refractory B‑ALL [25]. Fifty-one patients achieved a CR including 43 with MRD-negativity and 25 subsequently underwent allogeneic HSCT. The 24-month cumulative incidences of post HSCT relapses were 13.5 and 11.3% after CD19 and CD22-CAR‑T cell therapy without an increased risk of severe GvHD. Park and colleagues observed no difference in OS between patients who completed HSCT and those who did not when comparing outcomes of the 32 patients who achieved an MRD-negative CR following CAR‑T cell infusion [9]. In the ELIANA trial which included 75 patients with relapsed or refractory B‑ALL only 8 patients (9%) underwent allogeneic HSCT in remission after CAR‑T cell therapy [6]. In view of the small patient numbers and the many differences in study design and conduct, the question whether CAR‑T cells should be administered as bridging therapy to allogeneic HSCT or are sufficiently effective by themselves cannot be answered for certain. When patients have not undergone an allogeneic HSCT previously this follow-up therapy must at least be considered.
CAR‑T cells are clearly of benefit for treatment following relapse after allogeneic HSCT since in this patient population second transplant is associated with increased toxicity and low success rates [26]. In the ELIANA study, 61% of patients had relapsed/refractory B‑ALL after allogeneic HSCT [6]. T cells were successfully collected from patients after HSCT and high remission rates without GvHD have been reported [6, 9, 17]. Thus, previous HSCT does not impact outcomes after CD19-CAR‑T cell therapy.

Conclusions

During the last few years CAR‑T cells have been frequently used for bridging to allogeneic HSCT in patients with refractory or relapsed B‑ALL [25] since we currently are not able to distinguish those CAR‑T cell induced CRs that will persist without further therapy from those that are likely to be short lived. The choice to offer HSCT to patients following CAR‑T cell therapy relies on historical experience that transplantation has been the only curative option for patients with refractory or relapsed B‑ALL. Future improvements in CAR‑T cell constructs may allow longer term remissions without additional HSCT.

Conflict of interest

H.T. Greinix received honoraria as a speaker in scientific meetings from the companies Novartis and Celgene.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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