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Horm Metab Res 1997; 29(6): 317-321
DOI: 10.1055/s-2007-979043
Symposium Reports

© Georg Thieme Verlag Stuttgart · New York

Does Regulatory Protein Play a Role in Glucokinase Localization?

F. B. Reitz, L. Pagliaro
  • Center for Bioengineering, University of Washington, Seattle WA, U.S.A.
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Publication History

1996

1997

Publication Date:
23 April 2007 (online)

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Abstract

The enzyme glucokinase has recently been found to be largely responsible for glucose homeostatic responses of both the liver and pancreas. The mechanism(s) of these responses remains unknown but recent studies suggest that the intracellular localization of glucokinase, controlled by glucokinase regulatory protein, may be important. This protein is known to bind to and inhibit glucokinase in a phosphofructose-sensitive manner, and we present evidence for the interaction of these proteins with F-actin. Glucokinase regulatory protein gelled F-actin, and gelation was specifically inhibited by glucokinase and the regulatory protein effectors fructose-1-phosphate (F1P) and fructose-6-phosphate (F6P). These results suggest that glucokinase regulatory protein may play a role in metabolism-sensitive glucokinase localization in vivo.

Key words

Glucokinase Regulatory Protein - Glucokinase - F-Actin - Enzyme Localization

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