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Planta Med 1984; 50(4): 316-319
DOI: 10.1055/s-2007-969719
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Cumarinderivate als Schutzstoffe gegen die zytotoxische und mutagene Wirkung von 5-Methoxypsoralen und UV-A in Chlamydomonas reinhardii

Protective effects of some coumarin derivatives on the cytotoxic and mutagenic activity of 5-methoxypsoralen and UV-A irradiation in Chlamydomonas reinhardii Oskar Schimmer
  • Institut für Botanik und Pharmazeutische Biologie der Universität Erlangen-Nürnberg, Schloßgarten 4, D-8520 Erlangen, Bundesrepublik Deutschland
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Publication History

1983

1984

Publication Date:
26 February 2007 (online)

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Abstract

The protective effects of scopoletin and related coumarin derivatives on the cytotoxicity and mutagenicity of 5-methoxypsoralen plus UV-A were investigated in arg- cells of Chlamydomonas reinhardii.

Mutagenicity was determined by the number of Arg+ revenants induced by the treatment. The mutation frequency induced by 5-methoxypsoralen plus UV-A was substantially inhibited in a dose dependent fashion by the coumarin derivatives. The cytotoxicity was also inhibited in the presence of scopoletin.

The mutagenicity of 5-methoxypsoralen (1 µg/ml ≙ 4.63 µm) plus UV-A (2 kJ/m2) on arg- cells was reduced to about 60% by 5,7-dimethoxycoumarin, 73% by scopoletin and 95% by umbelliferone at concentrations of 5 µM. All three coumarin derivatives showed a stronger but more uniformly inhibiton of mutagenicity at higher concentration levels (50-100 µM).

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