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Planta Med 1998; 64(6): 541-545
DOI: 10.1055/s-2006-957510
Papers
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effect of Perilla frutescens and its Phenolic Constituents on Cultured Murine Mesangial Cell Proliferation

Toshiaki Makino1 , Takahiko Ono2 , Eri Muso2 , Gisho Honda1
  • 1Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
  • 2Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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Publication History

1997

1998

Publication Date:
01 February 2007 (online)

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Abstract

The inhibitory effects of Perilla frutescens and its phenolic constituents on cytokine-induced proliferation of murine cultured mesangial cells were investigated. DNA synthesis of mesangial cells stimulated by platelet derived growth factor (PDCF, 10ng/ml) or tumor necrosis factor (TNF)-α (lOO U/ml) was inhibited by the extract of P. frutescens (IC50 values, 3.3 and 1.4 µg/ml, respectively). The strength of the anti-proliferative activity was nearly equal in various chemotypes of P. frutescens. Caffeic acid, methyl caffeate, rosmarinic acid, and luteolin 7-O-glucuronide-6″-methyl ester were isolated as active constituents from the extract of the typical strain of P. frutescens, and their IC50 values for PDGF-induced mesangial cell proliferation were estimated as 26 µM, 2.6 µM, 1.8 µM, and 4.1 µM, respectively. We also compared the activities of related flavonoids previously isolated from P. frutescens, and luteolin had the highest anti-proliferative activity.

Key words

Perilla frutescens - Labiatae - mesangial cell - caffeic acid - rosmarinic acid - luteolin 7-O-glucuronide-6″-methyl ester

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