Download PDF
Thromb Haemost 1982; 47(02): 154-156
DOI: 10.1055/s-0038-1657152
Original Article
Schattauer GmbH Stuttgart

Synthesis of Thromboplastin Protein by a Murine Macrophage-Like Cell Line

T Lyberg
The Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Oslo, Norway
,
Ø Hetland
The Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Oslo, Norway
,
H Prydz
The Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received 17 November 1981

Accepted 01 March 1982

Publication Date:
13 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

The established murine macrophage cell line J-774.1 responds to endotoxin with synthesis of thromboplastin apoprotein. The response develops in the absence of added lymphocytes and there is no increased responsiveness in cocultures of J-774.1 cells and BALB/c lymphocytes. J-774.1 cells therefore do not depend on lymphocyte cooperation for their response to endotoxin.

Key words

Endotoxin - J-774.1 cells - Lymphocytes - Thromboplastin - TPA
 

  • References

  • 1 Rivers RPA, Hathaway WE, Weston WL. The endotoxin-induced coagulant activity of human monocytes. Br J Haematol 1975; 30: 311-316
    CrossrefPubMedGoogle Scholar
  • 2 Prydz H, Allison AC. Tissue thromboplastin activity of isolated human monocytes. Thromb Haemostas 1978; 39: 582-591
    PubMedGoogle Scholar
  • 3 Hiller E, Saal JG, Rietmüller G. Procoagulant activity of activated monocytes. Haemostasis 1977; 6: 347-350
    PubMedGoogle Scholar
  • 4 Van Ginkel CJW, Thørig L, Thompson J, Oh JIH, van Aken WG. Enhancement of generation of monocyte tissue thromboplastin by bacterial phagocytosis: possible pathway for fibrin formation on infected vegetations in bacterial endocarditis. Infect Immun 1979; 25: 388-395
    PubMedGoogle Scholar
  • 5 Prydz H. Thromboplastin activity of murine macrophages and human monocytes. Proceedings I. Intern. Workshop on the Heterogeneity of Mononuclear Phagocytes, Baden/Vienna. 1980
    PubMedGoogle Scholar
  • 6 Amlie E, Lyberg T, Kaplun A, Hetland Ø, Prydz H. Thromboplastin activity of mouse peritoneal macrophages. Thromb Res. in press
    PubMedGoogle Scholar
  • 7 Prydz H, Lyberg T. Tissue thromboplastin – Molecular and cellular biology. In Protides of the Biological Fluids. Peeters H. (ed). 28 241-244 Pergamon Press; 1980
    Google Scholar
  • 8 Bjørklid E, Storm E, Prydz H. The protein component of human brain thromboplastin. Biochem Biophys Res Commun 1973; 55: 969-976
    CrossrefPubMedGoogle Scholar
  • 9 Bjørklid E, Storm E. Purification and some properties of the protein component of tissue thromboplastin from human brain. Biochem J 1977; 165: 89-96
    CrossrefPubMedGoogle Scholar
  • 10 Edwards RL, Rickies FR, Bobrove AM. Mononuclear cell tissue factor: Cell of origin and requirements for activation. Blood 1979; 54: 359-370
    PubMedGoogle Scholar
  • 11 Levy GA, Edgington TS. Lymphocyte cooperation is required for amplification of macrophage procoagulant activity. J Exp Med 1980; 151: 1232-1244
    CrossrefPubMedGoogle Scholar
  • 12 Østerud B, Lindahl U, Seljelid R. Macrophages produce blood coagulation factors. FEBS Letters 1980; 120: 41-43
    CrossrefPubMedGoogle Scholar
  • 13 Hjort PF. Intermediate reactions in the coagulation of blood with tissue thromboplastin. Scand J Clin Lab Invest. 1957 9: suppl. 27.
    PubMedGoogle Scholar
  • 14 Stormorken H. Species differences of clotting factors in ox, dog, horse and man. Thromboplastin and proconvertin. Acta Physiol Scand 1957; 41: 301-324
    CrossrefPubMedGoogle Scholar
  • 15 Bøyum A. Isolation of mononuclear cells and granulocytes from human blood. J Clin Lab Invest. 1968 21: suppl. 97.
    PubMedGoogle Scholar
  • 16 Little C, Aurebekk B, Otnaess AB. Purification by affinity chromatography of Phospholipase C from Bacillus cereus. FEBS Letters 1975; 2: 175-179
    PubMedGoogle Scholar
  • 17 Hetland Ø, Prydz H. Phospholipase C from Bacillus cereus has sphingomyelinase activity. Scand J Clin Lab Invest. in press
    PubMedGoogle Scholar
  • 18 Markwell MAK, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87: 206-210
    CrossrefPubMedGoogle Scholar
  • 19 Prydz H, Lyberg T, Deteix P, Allison AC. In vitro stimulation of tissue thromboplastin (Factor III) activity in human monocytes by immune complexes and lectins. Thromb Res 1979; 15: 465-474
    CrossrefPubMedGoogle Scholar
  • 20 Ralph P, Prichard J, Cohn M. Reticulum cell sarcoma: an effector cell in antibody-dependent, cell-mediated immunity. J Immunol 1975; 114: 898-905
    PubMedGoogle Scholar
  • 21 Kaplan G, Mørland B. Properties of murine monocytic tumor cell-line J-774 in vitro. I. Morphology and endocytosis. Exp Cell Res 1978; 115: 53-61
    CrossrefPubMedGoogle Scholar