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Thromb Haemost 1998; 79(02): 395-399
DOI: 10.1055/s-0037-1614998
Letters to the Editor
Schattauer GmbH

Nickel Enhances Collagen-induced Platelet Activation Acting by Increasing the Organization of the Cytoskeleton

Fabio M. Pulcinelli
1   From the Dept. of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy; Pharmacology Dept. and Sol Sherry Thrombosis Center, Temple University, Philadelphia, PA, USA
,
Silvia Sebastiani
1   From the Dept. of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy; Pharmacology Dept. and Sol Sherry Thrombosis Center, Temple University, Philadelphia, PA, USA
,
Marzia Pesciotti
1   From the Dept. of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy; Pharmacology Dept. and Sol Sherry Thrombosis Center, Temple University, Philadelphia, PA, USA
,
Pasquale Pignatelli
1   From the Dept. of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy; Pharmacology Dept. and Sol Sherry Thrombosis Center, Temple University, Philadelphia, PA, USA
,
Pier Paolo Gazzaniga
1   From the Dept. of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy; Pharmacology Dept. and Sol Sherry Thrombosis Center, Temple University, Philadelphia, PA, USA
,
James L. Daniel
1   From the Dept. of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy; Pharmacology Dept. and Sol Sherry Thrombosis Center, Temple University, Philadelphia, PA, USA
› Author Affiliations
Further Information

Publication History

Received 10 March 1997

Accepted after resubmission 23 September 1997

Publication Date:
08 December 2017 (online)

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Summary

In keratinocytes, osteoclasts and enterocytes, Ni2+ acts as an agonist working through selective activation of the polyvalent cation-sensing receptor.

We report here that while Ni2+ alone had no direct ability to induce platelet aggregation or secretion, Ni2+ pretreatment produced these responses when platelets were stimulated with subthreshold concentrations of collagen. In addition, pretreatment with Ni2+ significantly enhanced collagen-induced phospholipase C activation and calcium mobilization. Platelet adhesion to collagen was increased and the inhibition of collagen-induced adhesion normally seen after cytochalasin D treatment was significantly diminished.

When Ni2+ was added to platelets alone, tyrosine phosphorylation of p60src was increased. Moreover, Ni2+ enhanced the amount of protein, especially actin, found in the low-speed Triton X-100 insoluble cyto-skeleton. Our results indicate that nickel, possibly acting via a platelet cation sensing receptor analogous to that which has been described in other cell types, may cause a rapid tyrosine kinase-dependent cyto-skeleton reorganization leading to enhanced adhesion of platelets to collagen and increasing collagen-dependent responses.

 

  • References

  • 1 Filvaroff E, Calautti E, Reiss M, Dotto GP. Functional evidence for an extracellular calcium receptor mechanism triggering tyrosine kinase activation associated with mouse keratinocyte differentiation. J Biol Chem 1994; 269: 21735-40.
    PubMedGoogle Scholar
  • 2 Shankar VS, Huang CLH, Adebanjo O, Simon B, Alam ASMT, Moonga BS, Pazianas M, Scott RH, Zaidi M. Effect of membrane potential on surface Ca2+ receptor activation in rat osteoclasts. J Cell Physiol 1995; 162: 1-8.
    CrossrefPubMedGoogle Scholar
  • 3 Shankar VS, Bax CMR, Bax BE, Alam ASMT, Pazianas M, Huang CLH, Zaidi M. Activation of the Ca2+ receptor on the osteoclasts by Ni2+ elicits cytosolic Ca2+ signals: Evidence for receptor activation and inactivation, intracellular Ca2+ redistribution and divalent cation modulation. J Cell Physiol 1993; 155: 120-9.
    CrossrefPubMedGoogle Scholar
  • 4 Brown EM, Gamba G, Riccardi D, Lombardi M, Butters R, Kifor O, Sun A, Hediger MH, Lytton J, Hebert SC. Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid. Nature 1993; 366: 575-80.
    CrossrefPubMedGoogle Scholar
  • 5 Riccardi D, Park JI, Lee WS, Gamba G, Brown EM, Hebert SC. Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor. Proc Natl Acad Sci USA 1995; 92: 131-5.
    CrossrefPubMedGoogle Scholar
  • 6 Sage SO, Reast R, Rink TJ. ADP evokes biphasic Ca2+ influx in fura-2-loaded human platelets. Biochem J 1990; 265: 675-80.
    CrossrefPubMedGoogle Scholar
  • 7 Mahaut-Smith MP, Sage SO, Rink TJ. Receptor-activated single channels in intact human platelets. J Biol Chem 1990; 265: 10479-83.
    PubMedGoogle Scholar
  • 8 Azula FJ, Alonso R, Marino A, Trueba M, Macarulla JM. Ni2+ impairs thrombin-induced signal transduction by acting on the agonist and/or receptor in human platelets. Am J Physiol 1993; 265: C1681-C8.
    CrossrefPubMedGoogle Scholar
  • 9 Aster RH, Jandel JH. Platelet sequestration in man. I. Methods. J Clin Invest 1964; 43: 834-55.
    CrossrefPubMedGoogle Scholar
  • 10 Born GVR. The aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962; 194: 927-9.
    PubMedGoogle Scholar
  • 11 Costa JJ, Murphy DL. Platelet 5-HT uptake and release stopped rapidly by formaldehyde. Nature 1975; 255: 405-7.
    CrossrefPubMedGoogle Scholar
  • 12 Holmsen H, Setkowsky-Dangelmaier CA. Adenine nucleotide metabolism of blood platelets. X. Formaldehyde stops centrifugation-induced secretion after A23187-stimulation and causes breakdown of metabolic ATP. Biochem Biophys Acta 1977; 497: 46-61.
    CrossrefPubMedGoogle Scholar
  • 13 Pulcinelli FM, Gazzaniga PP, Salganicoff L. Use of Zn-pyrophosphatase in the high-performance liquid chromatography analysis of cell extracts containing 32P-labelled inositol phosphates. J Chromatogr 1992; 575: 51-5.
    CrossrefPubMedGoogle Scholar
  • 14 Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 1985; 260: 3440-5.
    PubMedGoogle Scholar
  • 15 Smith JB, Dangelmaier C. Determination of platelet adhesion to collagen and the associated formation of phosphatidic acid and calcium mobilization. Anal Biochem 1990; 187: 173-8.
    CrossrefPubMedGoogle Scholar
  • 16 Smith JB, Dangelmaier C, Selak MA, Daniel J. Facile platelet adhesion to collagen requires metabolic energy and actin polimerization and evokes intracellular free calcium mobilization. J Cell Biochem 1991; 47: 54-61.
    CrossrefPubMedGoogle Scholar
  • 17 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
    CrossrefPubMedGoogle Scholar
  • 18 Smith JB, Selak MA, Dangelmaier C. Cytosolic calcium as a second messenger for collagen-induced platelet responses. Biochem J 1992; 288: 925-9.
    CrossrefPubMedGoogle Scholar
  • 19 Santoro SA. Identification of a 160,000 dalton platelet membrane protein that mediates the initial divalent cation-dependent adhesion of platelets to collagen. Cell 1986; 46: 913-20.
    CrossrefPubMedGoogle Scholar
  • 20 Niewenhuis HK, Akkerman JWN, Houdijk WPM, Sixma JJ. Human blood platelets showing no response to collagen fail to express surface glycoprotein Ia. Nature 1985; 318: 470-2.
    CrossrefPubMedGoogle Scholar
  • 21 Kunicki T, Nugent DJ, Staats SJ, Orchekowski RP, Wayner EA, Carter WG. The human fibroblast class II extracellular matrix receptor mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex. J Biol Chem 1988; 263: 4516-9.
    PubMedGoogle Scholar
  • 22 Daniel JL, Dangelmaier C, Smith JB. Evidence for a role for tyrosine phosphorylation of phospholipase C 2 in collagen-induced platelet cytosolic calcium mobilization. Biochem J 1994; 302: 617-22.
    CrossrefPubMedGoogle Scholar
  • 23 Blake RA, Schieven GL, Watson SP. Collagen stimulates tyrosine phosphorylation of phospholipase C- 2 but not phospholipase C-gamma1 in human platelets. FEBS Lett, 1994; 353: 212-6.
    CrossrefPubMedGoogle Scholar
  • 24 Keely PJ, Parise LV. The (2) (1) integrin is a necessary co-receptor for collagen-induced activation of syk and the subsequent phosphorylation of phospholipase C- 2 in platelets. J Biol Chem 1996; 271: 26668-76.
    CrossrefPubMedGoogle Scholar
  • 25 Arai M, Yamamoto N, Moroi M, Akamatsu N, Fukatake K, Tanoue K. Platelets with 10% of the normal amount of glycoprotein VI have an impaired response to collagen that results in a mild bleeding tendency. Br J Haematol 1995; 89: 124-30.
    CrossrefPubMedGoogle Scholar
  • 26 Ichinohe T, Takayama H, Ezumi Y, Arai M, Yamamoto N, Takahashi H, Okuma M. Collagen-stimulated activation of syk but not c-src is severely compromised in human platelets lacking membrane glycoprotein VI. J Biol Chem 1996; 272: 63-8.
    PubMedGoogle Scholar
  • 27 Gibbins J, Asselin J, Farnsdale R, Barnes M, Law C-L, Watson SP. Tyrosine phosphorylation of the Fc Receptor -chain in collagen-stimulated platelets. J Biol Chem 1996; 271: 18096-9.
    PubMedGoogle Scholar
  • 28 Kaplan KB, Sedlow JR, Morgan DO, Varmud HE. c-Src enhances the spreading of src-/- fibroblasts on fibronectin by a kinase-independent mechanism. Genes Dev 1995; 9: 1505-17.
    CrossrefPubMedGoogle Scholar
  • 29 Fox JEB, Lipfer L, Clark EA, Reynolds CC, Austin CD, Brugge JS. On the role of the platelet membrane skeleton in mediating signal transduction. J Biol Chem 1993; 268: 25973-84.
    PubMedGoogle Scholar
  • 30 Shankar VS, Bax CMR, Alam ASMT, Bax BE, Huang CL, Zaidi M. The osteoclast Ca2+ receptor is highly sensitive to activation by transition metal cations. Biochem Biophys Research Commun 1992; 187: 913-8.
    CrossrefPubMedGoogle Scholar
  • 31 Daniel JL, Dangelmaier C, Smith JB. Evidence that adhesion of electrically permeabilized platelets to collagen is mediated by guanine nucleotide regulatory proteins. Biochem J 1992; 286: 701-5.
    CrossrefPubMedGoogle Scholar