Separation of Free and Antibody-Bound Labelled Hormone by Kaolin in HGH Radioimmunoassay

P. Franchimont; J. J. Legros; B. Deconinck; A. Brunetti

doi:10.1055/s-0028-1095137

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Horm Metab Res 1969; 1(5): 218-220
DOI: 10.1055/s-0028-1095137

Originals

Separation of Free and Antibody-Bound Labelled Hormone by Kaolin in HGH Radioimmunoassay[*]

P. Franchimont¹ , J. J. Legros¹ [**] , B. Deconinck² , A. Brunetti³

¹Institute of Medicine, Department of Clinical Medicine and Pathology, University of Liège
²Service de Pediatrie, Hôpital Calmette, University of Lille
³Istituto di Semeiotica Medica, University of Padova

Further Information

Publication History

Publication Date:
08 January 2009 (online)

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Abstract

A rapid and easy separation of free HGH* from antibody bound HGH* is obtained with kaolin. Counting is performed on the total incubation volume. The HGH* which fails to adsorb to kaolin is in large measure damaged both physicochemically and immunologically. The serum protein concentration of the incubation mixture has little effect on the adsorption of free HGH* to kaolin. However, a sufficiently high concentration is needed to ensure that all the HGH*-Ab complexes will remain in the supernatant solution.

Key words

Human Growth Hormone Radioimmunoassay - Separation by Kaolin - Absorption of HGH

1 This work was supported by Grant No. 1013, Fonds de la Recherche Scientifique Medicale.

2 Research fellow of F.N.R.S.

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