01.04.2016 | Original Article
Poly(ADP-ribose) Polymerase inhibition improves wound healing in the colonic anastomoses of rats
Erschienen in: European Surgery | Ausgabe 2/2016Einloggen, um Zugang zu erhalten
Colonic anastomosis is a widely used surgical procedure and leads to morbidity and mortality with anastomotic leaks. It has been shown that PARP inhibition has beneficial effects against various pathological conditions. In this study, we aimed to evaluate the possible effects of 3-aminobenzamide (3-AB), a PARP-1 inhibitor, on experimental wound healing and tensile strength in the colonic anastomosis of rats.
A total of 14 Sprague-Dawley rats were divided into two random groups of equal number: Control and 3-AB groups. Control group was given standard left colon resection and end-to-end anastomosis; 3-AB group was given colonic resection + anastomosis + received 3-AB. 3-AB group received 20 mg/kg/day 3-AB intraperitoneally following surgical procedure and continued once a day for consecutive 4 days. A relaparatomy was given on the 5th day to evaluate malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), interleukin (IL)-10, IL-6, tumor necrosis factor-alpha (TNF-α), hydroxy (OH)-proline levels and anastomotic burst pressure.
The burst pressure values and the tissue OH-proline levels increased in the treatment group significantly compared with controls. Additionally, when compared the control group, tissue MDA, MPO, TNF-α and IL-10 levels were markedly decreased while tissue SOD and GSH-Px activities were statistically increased in the treatment group (p < 0.05).
Our data showed that 3-AB medication after colonic surgery increased the mechanical tissue strength of anastomotic line in rats. Consequently, these findings suggest that it would be possible to improve the anastomotic wound healing by using PARP inhibitors, and decrease the anastomotic complication rates.