Patient factors affecting culture of Helicobacter pylori isolated from gastric mucosal specimens
Section snippets
INTRODUCTION
A number of methods are used to detect H. pylori in endoscopic gastric mucosal specimens. One of them is culture. Since culture is costly and time-consuming procedure, requiring an experienced practitioner, not many microbiology laboratories in Poland perform it. For clinical purposes, the routine diagnosis of H. pylori infection of the stomach involves one or two simpler and cheaper tests (Campylobacter-like organisms test (CLO-test), urea breath test, stool antigen test, histology) [1].
MATERIALS AND METHODS
The study was conducted on a group of 265 patients of both genders, aged 19–79 years, hospitalized in the Department of Internal Medicine and Gastroenterology of the District Hospital due to abdominal complaints. Their final diagnosis was dyspepsia (221 patients), duodenal ulcer (32 patients), and gastric ulcer (12 patients). Exclusion criteria were as follows: taking antibiotics within the last 30 days, drinking alcohol within the last 7 days, and smoking cigarettes in the morning hours prior
RESULTS
The culture of H. pylori was performed in 265 patients, yielding 134 positive and 131 negative outcomes. In 5 culture positive patients, the result of the culture was in total inconsistency with CLO-test and histology. In 13 patients with positive culture, the inconsistency was partial – in 6 patients histology was negative and CLO-test positive whereas in 7 patients CLO-test was negative and histology positive (Tab. 1). In 15 culture negative patients, the culture result was in total
DISCUSSION
In the current study, total consistency of CLO-test and histological examination with positive and negative culture results was noted at 86.7% and 78.0%, respectively. Assuming that positive results in at least 2 tests prove the presence of H. pylori infection, all the positive culture group subjects except one had at least 2 other positive diagnostic tests. In only one patient, the positive result was confirmed by one accessory test, i.e. serological examination. Since culture was not the only
ACKNOWLEDGEMENTS
The study was supported by Medical University of Bialystok, grants no 3-22476 F, 3-22458 F, and 3-22981 F
REFERENCES (16)
- et al.
Methods to detect Helicobacter pylori: from culture to molecular biology
Helicobacter
(2007 Nov) - et al.
Current concept in the management of Helicobacter pylori: the Maastricht III Consensus Report
Gut
(2007 Jun) - et al.
Culturing Helicobacter pylori from clinical specimens: review of microbiologic methods
J Pediat Gastroenterol Nutr
(2003 May) - et al.
Rapid urease test in the management of Campylobacter pyloridis-associated gastritis
Am J Gastroenterol
(1987 Mar) The Sydney System: histological division
J Gastroenterol Hepatol
(1991 May-Jun)- et al.
Smoking habit and gastritis histology
Adv Med Sci
(2007) - et al.
Urease-positive bacteria other than Helicobacter pylori in human gastric juice and mucosa
Am J Gastroenterol
(2006 Aug) - et al.
The HOMER Study: the effect of increasing the dose of metronidazole when given with omeprazole and amoxicillin to cure Helicobacter pylori infection
Helicobacter
(2000 Dec)
Cited by (21)
Sixth Chinese National Consensus Report on Management of Helicobacter pylori Infection (Treatment Excluded)
2023, Chinese Journal of GastroenterologyFocusing on Helicobacter pylori infection in the elderly
2023, Frontiers in Cellular and Infection MicrobiologyAdvances on diagnosis of Helicobacter pylori infections
2023, Critical Reviews in MicrobiologyDetection of Helicobacter Pylori from Stomach Biopsies: A Rapid Culture Method
2022, Medical Forum MonthlyHelicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance
2022, Clinical Microbiology Reviews