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Effects of different cooking methods on health-promoting compounds of broccoli

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Abstract

The effects of five domestic cooking methods, including steaming, microwaving, boiling, stir-frying, and stir-frying followed by boiling (stir-frying/boiling), on the nutrients and health-promoting compounds of broccoli were investigated. The results show that all cooking treatments, except steaming, caused significant losses of chlorophyll and vitamin C and significant decreases of total soluble proteins and soluble sugars. Total aliphatic and indole glucosinolates were significantly modified by all cooking treatments but not by steaming. In general, the steaming led to the lowest loss of total glucosinolates, while stir-frying and stir-frying/boiling presented the highest loss. Stir-frying and stir-frying/boiling, the two most popular methods for most homemade dishes in China, cause great losses of chlorophyll, soluble protein, soluble sugar, vitamin C, and glucosinolates, but the steaming method appears the best in retention of the nutrients in cooking broccoli.

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References

  • Adebooye, O.C., Vijayalakshmi, R., Singh, V., 2008. Peroxidase activity, chlorophylls and antioxidant profile of two leaf vegetables (Solanum nigrum L. and Amaranthus cruentus L.) under six pretreatment methods before cooking. Int. J. Food Sci. Technol., 43(1):173–178.

    Article  CAS  Google Scholar 

  • AOAC (Association of Official Analytical Chemists), 1990. Official Method 985.33. Vitamin C (Reduced Ascorbic Acid) in Ready-to-feed Milk-based Infant Formula 2,6-Dichloroindophenol Titrimetric Method. In: Official Methods of Analysis. AOAC International, Washington DC, p.1108–1109.

    Google Scholar 

  • Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem., 72(1–2):248–254. [doi:10.1016/0003-2697(76)90527-3]

    Article  PubMed  CAS  Google Scholar 

  • Cieslik, E., Leszczynska, T., Filipiak-Florkiewicz, A., Sikora, E., Pisulewski, P.M., 2007. Effects of some technological processes on glucosinolate contents in cruciferous vegetables. Food Chem., 105(3):976–981. [doi:10.1016/j.foodchem.2007.04.047]

    Article  CAS  Google Scholar 

  • Ciska, E., Kozlowska, H., 2001. The effect of cooking on the glucosinolates content in white cabbage. Eur. Food Res. Technol., 212(5):582–587. [doi:10.1007/s002170100293]

    Article  CAS  Google Scholar 

  • Gliszczyńska-Świgło, A., Ciska, E., Pawlak-Lemanska, K., Chmielewski, J., Borkowski, T., Tyrakowska, B., 2006. Changes in the content of health-promoting compounds and antioxidant activity of broccoli after domestic processing. Food Addit. Contam., 23(11):1088–1098. [doi:10.1080/02652030600887594]

    Article  PubMed  CAS  Google Scholar 

  • Haughn, G.W., Davin, L., Giblin, M., Underhill, E.W., 1991. Biochemical genetics of plant secondary metabolites in Arabidopsis thaliana: the glucosinolates. Plant Physiol. 97(1):217–226. [doi:10.1104/pp.97.1.217]

    Article  PubMed  CAS  Google Scholar 

  • Hudson, D.E., Dalal, A., Lachance, P.A., 1985. Retention of vitamins in fresh and frozen broccoli prepared by different cooking methods. J. Food Qual., 8(1):45–50. [doi:10.1111/j.1745-4557.1985.tb00832.x]

    Article  CAS  Google Scholar 

  • Jia, C.G., Xu, C.J., Wei, J., Yuan, J., Yuan, G.F., Wang, B.L., Wang, Q.M., 2009. Effect of modified atmosphere packaging on visual quality and glucosinolates of broccoli florets. Food Chem., 114(1):28–37. [doi:10.1016/j.foodchem.2008.09.009]

    Article  CAS  Google Scholar 

  • Lee, S.K., Kader, A.A., 2000. Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biol. Technol., 20(3):207–220. [doi:10.1016/S0925-5214(00)00133-2]

    Article  CAS  Google Scholar 

  • Lin, C.H., Chang, C.Y., 2005. Textural change and antioxidant properties of broccoli under different cooking treatments. Food Chem., 90(1–2):9–15. [doi:10.1016/j.foodchem.2004.02.053]

    Article  CAS  Google Scholar 

  • Liu, X.Q., Li, Y.H., 2000. Epidemiological and nutritional research on prevention of cardiovascular disease in China. Br. J. Nutr., 84(6):S199–S203. [doi:10.1079/096582197388699]

    Article  PubMed  CAS  Google Scholar 

  • López-Berenguer, C., Carvajal, M., Moreno, D.A., Garcia-Viguera, C., 2007. Effects of microwave cooking conditions on bioactive compounds present in broccoli inflorescences. J. Agric. Food Chem., 55(24):10001–10007. [doi:10.1021/jf071680t]

    Article  PubMed  CAS  Google Scholar 

  • Masrizal, M.A., Giraud, D.W., Driskell, J.A., 1997. Retention of vitamin C, iron, and beta-carotene in vegetables prepared using different cooking methods. J. Food Qual., 20(5):403–418. [doi:10.1111/j.1745-4557.1997.tb00483.x]

    Article  CAS  Google Scholar 

  • Matusheski, N.V., Swarup, R., Juvik, J.A., Mithen, R., Bennett, M, Jeffery, E.H., 2006. Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane. J. Agric. Food Chem., 54(6):2069–2076. [doi:10.1021/jf0525277]

    Article  PubMed  CAS  Google Scholar 

  • Miglio, C., Chiavaro, E., Visconti, A., Fogliano, V., Pellegrini, N., 2008. Effects of different cooking methods on nutritional and physicochemical characteristics of selected vegetables. J. Agric. Food Chem., 56(1):139–147. [doi:10.1021/jf072304b]

    Article  PubMed  CAS  Google Scholar 

  • Nisha, P., Singhal, R.S., Pandit, A.B., 2004. A study on the degradation kinetics of visual green colour in spinach (Spinacea oleracea L.) and the effect of salt therein. J. Food Eng., 64(1):135–142. [doi:10.1016/j.jfoodeng.2003.09.021]

    Article  Google Scholar 

  • Oerlemans, K., Barrett, D.M., Suades, C.B., Verkerk, R., Dekker, M., 2006. Thermal degradation of glucosinolates in red cabbage. Food Chem., 95(1):19–29. [doi:10.1016/j.foodchem.2004.12.013]

    Article  CAS  Google Scholar 

  • Roe, J.H., 1955. The determination of sugar in blood and spinal fluid with anthrone reagent. J. Biol. Chem., 212:335–343.

    PubMed  CAS  Google Scholar 

  • Rosa, E.A.S., Heaney, R.K., 1993. The effect of cooking and processing on the glucosinolate content: studies on 4 varieties of Portuguese cabbage and hybrid white cabbage. J. Sci. Food Agric., 62(3):259–265. [doi:10.1002/jsfa.27406 20309]

    Article  CAS  Google Scholar 

  • Rungapamestry, V., Duncan, A.J., Fuller, Z., Ratcliffe, B., 2006. Changes in glucosinolate concentrations, myrosinase activity, and production of metabolites of glucosinolates in cabbage (Brassica oleracea var. capitata) cooked for different durations. J. Agric. Food Chem., 54(20):7628–7634. [doi:10.1021/jf0607314]

    Article  PubMed  CAS  Google Scholar 

  • Rungapamestry, V., Duncan, A.J., Fuller, Z., Ratcliffe, B., 2007. Effect of cooking Brassica vegetables on the subsequent hydrolysis and metabolic fate of glucosinolates. Proceed. Nutr. Soc., 66(1):69–81. [doi:10.1017/S0029665107005319]

    Article  CAS  Google Scholar 

  • Schreiner, M.C., Peters, P.J., Krumbein, A.B., 2006. Glucosinolates in mixed-packaged mini broccoli and mini cauliflower under modified atmosphere. J. Agric. Food Chem., 54(6):2218–2222. [doi:10.1021/jf0525636]

    Article  PubMed  CAS  Google Scholar 

  • Serrano, M., Martinez-Romero, D., Guillen, F., Castillo, S., Valero, D., 2006. Maintenance of broccoli quality and functional properties during cold storage as affected by modified atmosphere packaging. Postharvest Biol. Technol., 39(1):61–68. [doi:10.1016/j.postharvbio.2005.08.004]

    CAS  Google Scholar 

  • Sikora, E., Cieslik, E., Leszczynska, T., Filipiak-Florkiewicz, A., Pisulewski, P.M., 2008. The antioxidant activity of selected cruciferous vegetables subjected to aquathermal processing. Food Chem., 107(1):55–59. [doi:10.1016/j. foodchem.2007.07.023]

    Article  CAS  Google Scholar 

  • Song, L.J., Thornalley, P.J., 2007. Effect of storage, processing and cooking on glucosinolate content of Brassica vegetables. Food Chem. Toxicol., 45(2):216–224. [doi:10.1016/j.fct.2006.07.021]

    Article  PubMed  CAS  Google Scholar 

  • Traka, M., Mithen, R., 2009. Glucosinolates, isothiocyanates and human health. Phytochem. Rev., 8(1):269–282. [doi:10.1007/s11101-008-9103-7]

    Article  CAS  Google Scholar 

  • Turkmen, N., Poyrazoglu, E.S., Sari, F., Velioglu, Y.S., 2006. Effects of cooking methods on chlorophylls, pheophytins and colour of selected green vegetables. Int. J. Food Sci. Technol., 41(3):281–288. [doi:10.1111/j.1365-2621.2005.01061.x]

    Article  CAS  Google Scholar 

  • Vallejo, F., Tomas-Barberan, F.A., Garcia-Viguera, C., 2002. Glucosinolates and vitamin C content in edible parts of broccoli florets after domestic cooking. Eur. Food Res. Technol., 215(4):310–316. [doi:10.1007/s00217-002-0560-8]

    Article  CAS  Google Scholar 

  • Verkerk, R., Dekker, M., 2004. Glucosinolates and myrosinase activity in red cabbage (Brassica oleracea L. var. Capitata f. rubra DC.) after various microwave treatments. J. Agric. Food Chem., 52(24):7318–7323. [doi:10.1021/jf0493268]

    Article  PubMed  CAS  Google Scholar 

  • Verkerk, R., Schreiner, M., Krumbein, A., Ciska, E., Holst, B., Rowland, I., Schrijver, R.D., Hansen, M., Gerhäuser, C., Mithen, R., Dekker, M., 2008. Glucosinolates in Brassica vegetables: the influence of the food supply chain on intake, bioavailability and human health. Mol. Nutr. Food Res., online. [doi:10.1002/mnfr.200800065]

  • Volden, J., Borge, G.I.A., Bengtsson, G.B., Hansen, M., Thygesen, I.E., Wicklund, T., 2008a. Effect of thermal treatment on glucosinolates and antioxidant-related parameters in red cabbage (Brassica oleracea L. ssp. capitata f. rubra). Food Chem., 109(3):595–605. [doi:10.1016/j.foodchem.2008.01.010]

    Article  CAS  Google Scholar 

  • Volden, J., Wicklund, T., Verkerk, R., Dekker, M., 2008b. Kinetics of changes in glucosinolate concentrations during long-term cooking of white cabbage (Brassica oleracea L. ssp. capitata f. alba). J. Agric. Food Chem., 56(6):2068–2073. [doi:10.1021/jf0731999]

    Article  PubMed  CAS  Google Scholar 

  • Zhang, D.L., Hamauzu, Y., 2004. Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chem., 88(4):503–509. [doi:10.1016/j.foodchem.2004.01.065]

    Article  CAS  Google Scholar 

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Correspondence to Qiao-mei Wang.

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Project supported by the High-Tech R&D Program (863) of China (No. 2008AA10Z111), the National Natural Science Foundation of China (No. 30320974), the Fok Ying Tong Education Foundation of China (No. 104034), and the Program for New Century Excellent Talents in the University of China (No. NCET-05-0516)

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Yuan, Gf., Sun, B., Yuan, J. et al. Effects of different cooking methods on health-promoting compounds of broccoli. J. Zhejiang Univ. Sci. B 10, 580–588 (2009). https://doi.org/10.1631/jzus.B0920051

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  • DOI: https://doi.org/10.1631/jzus.B0920051

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