Abstract
The aim of the present study is to evaluate the status of plasma essential trace element selenium (Se), manganese (Mn), copper (Cu), zinc (Zn), and iron (Fe) concentrations and the effect of these elements on oxidative status in patients with childhood asthma. Plasma Se, Mn, Cu, and Zn concentrations were determined by atomic absorption spectrophotometry (AAS) and Fe concentrations, malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined by the colorimetric method. The plasma MDA/TAC ratio was calculated as an index of oxidative status. Plasma albumin levels were measured to determine nutritional status. Plasma Fe concentrations, MDA levels and the MDA/TAC ratio were significantly higher (p<0.001, p<0.001, and p<0.01, respectively) and Se and Mn concentrations and TAC were lower (p<0.01, p<0.05, and p<0.01, respectively) in patients when compared to the healthy subjects. Plasma Zn, Cu, and albumin levels were not found to be significantly different in patients and controls (p>0.05). There were positive relationships between plasma MDA and Fe (r=0.545, p<0.001) and TAC and Se (r=0.485, p<0.021), and a negative correlation between TAC and MDA values (r= −0.337, p<0.031) in patients with childhood asthma. However, there was no correlation between these trace elements and albumin content in patient groups. These observations suggest that increased Fe and decreased Se concentrations in patients with childhood asthma may be responsible for the oxidant/antioxidant imbalance.
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J. Bounquest, P. K. Jeffery, W. W. Busse, et al., Asthma: from broncocontruction to airways inflammation and remodeling, Am. Rev. Respir. Dis. 145, 911–917 (2000).
N. M. Clark, R. W. Brown, E. Parker, et al., Childhood asthma, Environ Health Res. 107, 421–429 (1999).
P. J. Barnes, Reactive oxygen species and airflow inflammation, Free Radical Biol. Med. 9, 235–243 (1990).
W. MacNee, Oxidative strees and lung inflammation in airways disease, Eur. J. Pharm. 429, 195–207 (2001).
N. L. Misso, K. A. Powers, R. L. Gillon, et al., Reduced platelet glutathione peroxidase activity and plasma selenium concentration in atopic asthmatic patients, Clin. Exp. Allergy 26, 838–847 (1996).
L. J. Smith, M. Shamsuddin, P. H. Sporn, et al., Reduced superoxide dismutase in lung cells of patients with asthma, Free Radical Biol. Med. 22, 1301–1307 (1997).
A. Kocyigit, E. Ozcan, and S. Gür, Effects of tobacco smoking on plasma selenium, zinc, copper and iron concentrations and related antioxidative enzyme activities, Clin. Biochem. 34, 629–633 (2001).
B. Halliwell and J. M. Gutteridge, Role of free radicals and catalytic metal ions in human disease: an overview, Methods Enzymol. 186, 1–85 (1990).
J. M. Gutteridge, Lipid peroxidation and antioxidant as biomarkers of tissue damage, Clin. Chem. 41, 1819–1828 (1995).
W. MacNee, Oxidative stress and lung inflammation in airways disease, Eur. J. Pharm. 429, 195–207 (2001).
D. Milde, O. Novak, V. Stuka, et al., Plasma levels of selenium, manganese, copper, and iron in colorectal cancer patients, Biol. Trace. Element Res. 79, 107–114 (2001).
K. Yagi, Simple assay for the level of total lipid peroxides in serum or plasma, Methods Mol. Biol. 108, 101–106 (1998).
D. Koracevic, G. Koracevic, V. Djordjevic, et al., Method for measurements of antioxidant activity in human fluids, J. Clin. Pathol. 54, 356–361 (2001).
P. J. Barnes, ROS and airflow inflammation, Free Radical Biol. Med. 9, 235–243 (1990).
R. P. Bowler and J. D. Crapo, Oxidative stress in airways: is there a role for extracellular superoxide dismutase, Am. J. Respir. Crit. Care Med. 166, 38–43 (2002).
R. S. Kalathinkal, S. S. Kumar, and S. Rajaje, Excessive free radical generation in the blood of children suffering from asthma, Clin. Chim. Acta 305, 107–114 (2001).
J. M. Gutteridge, Lipid peroxidation and antioxidants as biomarkers of tissue damage, Clin. Chem. 41, 1819–1828 (1995).
I. Rahman and W. MacNee, Role of oxidant/antioxidant in smoking-induced lung diseases, Free Radical Biol. Med. 21, 669–682 (1996).
P. Aisen, G. Cohen, and J. O. Kang, Iron toxicosis, Int. Rev. Exp. Pathol. 31, 1–46 (1990).
J. M. Gutteridge and B. Halliwell, Free radicals and antioxidants in the year 2000. A historical look to the future, Ann NY Acad. Sci. 899, 136–147 (2000).
K. L. Maier, How the lung deals with antioxidants, Eur. Respir. J. 6, 334–336 (1993).
J. Stone, L. Hinks, R. Beasley, et al., Reduced selenium status of patients with asthma, Clin. Sci. 77, 495–500 (1989).
A. Flatt, N. Pearce, C. Thomson, et al., Reduced selenium in asthmatic subjects in New Zealand, Thorax 45, 95–99 (1990).
L. Hasselmark, R. Malmgren, O. Zeterstrom, et al., Selenium supplementation in intrinsic asthma, Allergy 48, 30–36 (1993).
R. Moreno-Reyes, C. Suetens, F. Mathieu, et al., Kashin-Beck osteoathropathy in rural Tibet in relation to selenium and iodine status, N. Engl. J. Med. 339, 1112–1120 (1998).
ISAAC Steering Committee, Worldwide variation in prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and atopic eczema: ISSAC, Lancet 351, 1225–1232 (1997).
A. Southar, A. Seaton, and K. Brown, Bronchial reactivity and dietary antioxidants, Thorax 52, 166–170 (1997).
J. E. Albina, C. D. Mills, W. L. Henry, et al., Temporal expression of different pathways of l-arginine metabolism in healing wounds, J. Immunol. 144, 3877–3880 (1994).
P. J. Barnes, Nitric oxide and asthma, Res. Immunol. 146, 698–702 (1995).
K. Ashutosh, Nitric oxide and asthma: a review, Curr. Opin. Pulmon. Med. 6, 21–25 (2000).
F. H. Gou, S. A. Comhair, S. Zheng, et al., Molecular mechanisms of increased NO (NO) in asthma: evidence for transcriptional and posttranslational regulation of NO synthesis, J. Immunol. 164, 5870–5980 (2000).
A. Bast, G. Haenen, and C. Doelman, Oxidants and antioxidants, Am. J. Med. 91, 2–13 (1991).
M. S. el-Kholy, M. A. Gas Allah, S. el-Shimi, et al., Zinc and copper status in children with bronchial asthma and atopic dermatitis, J. Egypt. Public Health. Assoc. 65, 657–681 (1990).
F. Akinkube and S. Ette, Role of zinc, copper and ascorbic acid in some common clinical pediatric problems, J. Trop. Paediatr. 33, 337–342 (1987).
H. Vural, K. Uzun, E. Uz, et al., Concentrations of copper, zinc and various elements in serum of patients with bronchial asthma, J. Trace. Element Med. Biol. 14, 88–91 (2000).
J. L. Malo, A. Cartier, and J. Dolovich, Occupational asthma due to zinc, Eur. Respir. J. 6, 447–450 (1993).
J. M. Freitas and R. Meneghini, Iron and its sensitive balance in the cell, Mutat. Res. 475, 153–159 (2001).
T. Nunoshiba, F. Obata, A. C. Boss, et al., Role of iron and superoxide for generation of hydroxyl radical, oxidative DNA lesions, and mutagenesis in Escherichia coli, J. Biol. Chem. 274, 34,832–34,837 (1999).
L. S. Greene, Asthma and oxidant stress: nutritional, environmental, and genetic risk factors, J. Am. Coll. Nutr. 14, 317–324 (1995).
J. J. Bullen, H. J. Rogers, and E. Griffiths, Role of iron in bacterial infection, Curr. Topics Microbiol. Immunol. 80, 1–35 (1978).
E. D. Weinberg, Iron and susceptibility to infectious disease, Science 184, 952–956 (1974).
P. Nafstad, P. Magnus, and J. J. Jaakkola, Early respiratory infections and childhood asthma, Paediatrics 106, 38–43 (2000).
L. C. von Hertzen, Puzzling associations between childhood infections and the later occurrence of asthma and atopy, Ann. Med. 32, 397–400 (2000).
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Kocyigit, A., Armutcu, F., Gurel, A. et al. Alterations in plasma essential trace elements selenium, manganese, zinc, copper, and iron concentrations and the possible role of these elements on oxidative status in patients with childhood asthma. Biol Trace Elem Res 97, 31–41 (2004). https://doi.org/10.1385/BTER:97:1:31
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DOI: https://doi.org/10.1385/BTER:97:1:31