http://orcid.org/0000-0002-6480-3422
Figures
Abstract
The prevalence of maternal obesity and gestational diabetes mellitus (GDM) is increasing rapidly. Probiotics supplementation have been shown to improve metabolic health in humans. In our study, we aimed to evaluate the effects of probiotics supplementation on metabolic health and pregnancy complications in pregnant women. The literature search, data extraction and quality assessment were performed, and data were synthesized in accordance with standardized guidelines. Ten randomized clinical trials with eligible data were included in our meta-analysis. For pregnant women with GDM, we found negative correlations between probiotics supplementation and fasting serum insulin (OR -2.94, 95%CI [-5.69, -0.20], p = 0.04) and homoeostasis model assessment for insulin resistance (HOMA-IR) (OR -0.65, 95%CI [-1.18, -0.11], p = 0.02). There were no significant correlations between probiotics supplementation and lipid levels in women with GDM, including total cholesterol (OR -2.72, 95%CI [-17.18, 11.74], P = 0.71), high density lipoprotein cholesterol (HDL-c) (OR -0.29, 95%CI [-3.60, 3.03], P = 0.87), low density lipoprotein cholesterol (LDL-c) (OR -0.38, 95%CI [-18.54, 17.79], P = 0.97), or triglycerides (OR -12.83, 95%CI [-36.63, 10.97], P = 0.29). For healthy pregnant women, probiotics supplementation were negatively associated with fasting serum insulin (OR -3.76, 95%CI [-4.29, -3.23], P < 0.00001) and HOMA-IR (OR -0.57, 95%CI [-1.08, -0.06], p = 0.03). However, no significant correlations were observed between probiotics supplementation and fasting plasma glucose (FPG) (OR -2.02, 95%CI [-5.56, 1.52], p = 0.26). Thus, our study revealed that probiotics supplementation during pregnancy have beneficial effects on glucose metabolism, rather than lipid metabolism among pregnant women.
Citation: Zheng J, Feng Q, Zheng S, Xiao X (2018) The effects of probiotics supplementation on metabolic health in pregnant women: An evidence based meta-analysis. PLoS ONE 13(5): e0197771. https://doi.org/10.1371/journal.pone.0197771
Editor: Cheryl S. Rosenfeld, University of Missouri Columbia, UNITED STATES
Received: January 9, 2018; Accepted: May 8, 2018; Published: May 21, 2018
Copyright: © 2018 Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This study was sponsored by the National Key Research and Development Program of China (2017YFC1309603, 2016YFA0101002), and the National Natural Science Foundation of China (No. 81570715); Beijing Municipal Natural Science Foundation (No. 7184252), the Fund for Fostering Young Scholars of Peking University Health Science Center (No. BMU2017PY008), China Scholarship Council of the Ministry of Education (No. 201506210378), Postgraduate Student Innovation Fund in Peking Union Medical College (No. 2013-1002-33). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
The prevalence of obesity and type 2 diabetes mellitus (T2DM) in adulthood is increasing dramatically [1]. However, the pathogenesis of diabetes mellitus has not been fully demonstrated. Increasing evidence shows that maternal environment, especially metabolic status during pregnancy, is a critical element determining the development of metabolic diseases, such as obesity and T2DM in offspring [2–4]. It is estimated that one in six births is affected by gestational diabetes mellitus (GDM) in 2017 [1]. About 50% women with childbearing ages and 20–25% pregnant women in Europe were overweight and obese [5]. Obese pregnant women are at higher risks of developing GDM, that can affect metabolic health of mothers and newborns.
Obese pregnant women and women with GDM are associated with higher risks of maternal and fetal morbidity and mortality [6]. For the mothers, it can increase the susceptibility of undergoing a caesarian section, preeclampsia and the development of T2DM postpartum [6–8]. For their fetuses, it increases the risks of macrosomia, polyhydramnios, shoulder dystocia, preterm birth and neonatal hypoglycemia [6–8]. Offspring of women with GDM may be more likely to develop obesity, insulin resistance and T2DM in adulthood [9]. In addition, maternal obesity is associated with neonatal adiposity and obesity in childhood. The programming effect was known as “Developmental Origins of Health and Disease (DOHaD)” hypothesis, and it has now been widely accepted [10, 11]. Therefore, obese pregnant women and women with GDM not only increase the incidecne of adverse pregnancy outcomes, but also have long-term effects on metabolic health of mothers and their offspring.
Lifestyle interventions, including diet and exercise have been demonstrated to prevent against maternal obesity and GDM. However, it has proven to be challenging, with low compliance and limited efficacy [12]. In recent years, gut microbiota is shown to be associated with obesity and several metabolic diseases [13]. It is related with developmental biology, including both prenatal and postnatal growth [14]. Probiotics supplementation have been shown to regulate microbiota dysbiosis, and probiotics are emerging as an effective intervention to improve whole body health in humans, and even in term and preterm infants [15, 16]. However, its roles in regulating metabolic health during pregnancy remain unclear, especially in obese pregnant women and women with GDM. Therefore, our study was aimed to determine the effects of probiotics supplementation on metabolic health in pregnant women, including healthy pregnant women, obese pregnant women and women with GDM.
Materials and methods
PRISMA guideline and PICO principle
This systematic review has been registered in PROSPERO titled as “The effects of probiotics supplementation on glucose metabolic health in pregnancy women” (register number: CRD42017060312). The review was conducted and reported in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guideline [17] and PICO principle, including P-Population: pregnant women, including healthy pregnant women, obese pregnant women and women with GDM; I-Intervention: probiotics supplementation; C-Comparison: metabolic health involving probiotics supplementation as an intervention during pregnancy, as compared with placebo; O-Outcome: changes in metabolic parameters and pregnancy outcomes with probiotics supplementation.
All the literature search, data extraction and quality assessment were performed independently and in duplicate by two authors (QYF and SZ) using a standardized approach. Any disagreements were documented and resolved by discussion between data collectors along with the principal investigator (JZ and XHX).
Data sources, search strategy, and selection criteria
Pubmed, Embase, Cochrane Library and Clinicaltrials.gov databases were systematically screened for relevant studies. All literatures were published updated to February 2017 and the language was limited to English. The main search terms were a combination of MESH terms and text words for probiotics, pregnancy and metabolic parameters, with the following terms: “probiotics” OR “bacteria” AND “pregnancy” OR “gestation” OR “parturition” AND “glucose” OR “insulin” OR “HbA1c” OR “glycosylated hemoglobin A1c” OR “glycemic control” OR “metabolism”. All the articles, including conference abstracts were reviewed. All the literatures were reviewed and additional relevant references quoted in searched articles were also screened. All the literatures were managed by Endnote X7 software.
All studies about changes in metabolic parameters before and after probiotics supplementation in pregnant women were screened. In our study, only randomized clinical trials (RCTs) were included, since RCT provides relatively strong evidence for the efficacy of clinical trials [18]. The studies that met the following criteria were included: (1) studies about the effects of probiotics on pregnancy outcomes; (2) measured glucose and lipid metabolism parameters; (3) sufficient data for evaluation. Studies were excluded based on the following criteria: (1) studies in which clinical outcomes of pregnancy could not be ascertained; (2) observational studies; (3) preclinical studies; (4) reviews or conference abstracts or case reports or editorials or book chapters.
Data extraction
Two authors (JZ and QYF) independently screened the title and abstract of each article for the relevance of subjects, quality of clinical trial and eligibility for inclusion. The following information was collected from each included study using a standardized protocol and reporting form: first author, year of publication, country, study design, subjects, probiotic interventions (including probiotics species, probiotics counts measured by colony-forming unit (cfu)), intervention duration, sample size, mean age, primary outcome and secondary outcomes that passed the two rounds of screening. The detailed information was shown in Table 1.
Assessment of study quality
Two authors (QYF and SZ) independently screened the included articles and assessed the study quality [19]. Ten criteria are generally used to assess the sufficiency of reporting, including: (1) Was the randomization method appropriate? (2) Was the allocation sequence concealed? (3) Were the participants blind to the intervention? (4) Were the outcome assessors blind to the intervention? (5) Was the outcome measurement performed in the same manner? (6) Were similarly trained individuals administering the intervention across groups? (7) Were all the withdrawals described? (8) Were all originally randomized participants analyzed in the groups they were assigned to? (9) Was clustering at the group level accounted for in the analyses? (10) Were the groups similar at baseline?
For each of these criteria, it indicated as a “Yes” judged as fulfilling the criterion, or indicated as a “No” for not fulfilling it, or indicated as “Not Reported (NR)” due to insufficient information. If the article has included a complete description regarding the process and outcome of each criterion, it can be designated as “Yes”. If the investigators would be unable to replicate the process based on unclear information, due to insufficient information, it was designated as a “NR” for that criterion. A complete lack of reporting or an erroneous method was marked as “No”. The evaluation of risk-of-bias criteria for each study was shown in Table 2.
Statistical analysis
RevMan 5.3 software was used for this meta-analysis. The Cochran's Q test, and I2 test were all performed to judge the heterogeneity among the studies. Heterogeneity was also considered to be significant at P<0.1 for the Q statistic. I2 values of 25%, 50% and 75% corresponded to low, moderate and high levels of heterogeneity, respectively [20]. The selection of fixed-effects model or random-effects model was depended on the size of the heterogeneity among the included studies [21]. Sensitivity analysis was performed by successively excluding the low quality studies to assess the stability of the outcomes [22]. Pooled odds ratios (ORs) were reported with 95% confidence intervals (CIs), and a two-tailed P < 0.05 was considered statistically significant for all analyses.
Results
Studies included and participant characteristics
Fig 1 summarizes the selection process of eligible studies. We identified 88 potentially eligible literatures and 68 were kept after removing duplicates. 44 articles were excluded due to preclinical studies, reviews or conference abstracts or case reports or editorials or book chapters or observational studies. 24 potential studies were further reviewed after reading the full article. Next, 14 articles were excluded based on the outcomes of interest, ongoing clinical trials and inaccessible for full paper. Finally, 10 RCTs with eligible data were included in the systematic review [23–32]. Of the ten studies, three studies were from the same study cohort [23–25], and two studies were from another study cohort [27, 31]. Of the ten RCTs, five studies were aimed to evaluate probiotics and its effects on metabolic health in healthy pregnant women [23–27]. Only one study was about the effect of probiotics on metabolic health in obese pregnant women [28]. The remaining four studies aimed to assess the effect of probiotics supplementation on metabolic health in women with GDM [29–32]. The enrollment sample size ranged from 60 to 256 subjects. The detailed characteristics of the studies are shown in Table 1, and the outcomes of each study are shown in Table 3.
RCT, randomized controlled trial; GDM, gestational diabetes mellitus.
Probiotics and its effectiveness in women with GDM
Four studies were included to assess the effect of probiotics supplementation on metabolic health in women with GDM [29–32]. For glucose metabolism, comprehensive integration and analyses revealed negative correlations between probiotics supplementation and fasting serum insulin (OR -2.94, 95%CI [-5.69, -0.20], p = 0.04) and homoeostasis model assessment for insulin resistance (HOMA-IR) (OR -0.65, 95%CI [-1.18, -0.11], p = 0.02). However, no significant correlations were observed between probiotics supplementation and fasting plasma glucose (FPG) (OR -3.24, 95%CI [-8.77, 2.30], p = 0.25), and quantitative insulin sensitivity check index (QUICKI) index (OR 0.01, 95%CI [0.00, 0.02], p = 0.05) (Table 4). Among the four studies, two studies assessed the effects of probiotics supplementation on lipid metabolism in women with GDM. However, there were no significant correlations between probiotics supplementation and lipid levels in women with GDM, including total cholesterol (OR -2.72, 95%CI [-17.18, 11.74], P = 0.71), high density lipoprotein cholesterol (HDL-c) (OR -0.29, 95%CI [-3.60, 3.03], P = 0.87), low density lipoprotein cholesterol (LDL-c) (OR -0.38, 95%CI [-18.54, 17.79], P = 0.97), or triglycerides (OR -12.83, 95%CI [-36.63, 10.97], P = 0.29) (Table 4). Among the four studies, two studies evaluated the effects of maternal probiotics supplementation on pregnancy outcomes. Lindsay et al. showed that there was no difference in neonatal anthropometry, assessed by absolute birth weight, birth weight centile, small or large for gestational age, macrosomia, head circumference or length [30]. Karamali et al. also found no difference in mean weight, height, head circumference, caesarean section rate or fetal macrosomia in newborns [31]. Overall, these results demonstrated that probiotic supplementations had beneficial effects on glycemic control, rather than lipid metabolism in women with GDM.
Probiotics and its effectiveness in healthy pregnant women
There are five RCTs about probiotics supplementation in healthy pregnant women and one research group had three publications on various metabolic endpoints of mothers. For glucose metabolism, comprehensive integration and analyses revealed significant correlations between probiotics supplementation and lower fasting serum insulin (OR -3.76, 95%CI [-4.29, -3.23], P < 0.00001) and HOMA-IR (OR -0.57, 95%CI [-1.08, -0.06], p = 0.03). However, no significant correlations were observed between probiotics supplementation and FPG (OR -2.02, 95%CI [-5.56, 1.52], p = 0.26) (Table 4). Only one study examined the infant health outcomes and found that there were no significant differences in prenatal or postnatal growth rates. This study supported the evidence that probiotic supplementation during pregnancy had beneficial effects on glucose metabolism in healthy pregnant women.
Probiotics and its effectiveness in obese pregnant women
One study was included to investigate the effect of a probiotics supplementation on metabolic health in obese pregnant women [28]. A total of 138 women were recruited in the study and randomly assigned to receive either a daily probiotic (Lactobacillus salivarius UCC118 (109 cfu)) or a placebo capsule from 24 to 28 weeks of gestation in addition to routine antenatal care. The primary outcomes were maternal FPG concentration changes, with the incidence of impaired glycemia including IGT or GDM as a secondary outcome. There were no differences in the incidence of impaired glycemia, birth weight, or pregnancy outcomes [28]. For infant health outcomes, there were no differences in number of large-for-gestational-age babies, admission to the neonatal intensive care unit, gestational age at delivery or Apgar score. The results can be explained by that there were only 15 cases of IGT and 6 cases of GDM among the participants, limiting the statistical power of the study.
Discussion
With the increasing prevalence of maternal obesity and GDM, it places enormous burden on individual and public health. Preventing obesity, insulin resistance and hyperglycemia during pregnancy shows pronounced benefits for metabolic health [33]. Currently, lifestyle interventions, including diet and exercise, have been demonstrated to prevent and treat GDM [34]. However, compliance with these interventions is low and the efficacy is limited. It showed that a behavioral intervention (including diet and physical activity) in obese pregnant women was not adequate to prevent the incidence of GDM, or to reduce the incidence of large-for-gestational-age infants [35]. Han et al. reviewed 19 trials with 1398 women with GDM and showed that no clear differences were observed among different types of dietary advice for women with GDM [36]. Other management practices, such as medication therapy for GDM are expensive but also do not always reduce the incidence of GDM [12].
According to the World Health Organization, probiotics can confer health benefits on the host microorganisms. Therefore, our systematic review aimed to assess the effects of probiotics supplementation on metabolic health in the pregnant women, including healthy pregnant women, obese pregnant women and women with GDM. Ten RCTs were included in the final systematic review. Five studies [23–27] were aimed to evaluate probiotics and its effects on metabolic health in healthy pregnant women. It demonstrated that probiotic supplementation during pregnancy had beneficial effects on glucose metabolism. One study [28] was about probiotics and metabolic health in obese pregnant women. However, there were no differences in metabolic variables or pregnancy outcomes in obese pregnant women, that may be due to the limited number of relevant studies. The remaining four studies [29–32] aimed to assess the effect of probiotics supplementation on metabolic health in pregnant women with GDM, and showed that probiotic supplementations had beneficial effects on glycemic control, rather than lipid metabolism among women with GDM. Taylor et al. reviewed the effects of probiotics on metabolic outcomes in pregnant women with GDM. Consistently, it also showed that improved glucose metabolism with a significant reduction in HOMA-IR was observed following probiotic supplementation [37]. Taken all together, probiotics supplementation during pregnancy have beneficial effects on metabolic health among pregnant women, including women with GDM, and even healthy pregnant women. Among the included ten studies, only four studies have evaluated the effects of probiotics supplementation on birth weight. However, the sample sizes were relatively small. Thus, the effects of probiotics supplementation on infant health outcomes are uncertain. More studies with larger sample sizes about the effects of probiotics supplementation on birth weight are needed.
In addition, the dose or CFU of a probiotic is an important factor for the efficacy of probiotics supplementation on metabolic health in pregnant women. Among the ten RCTs, the dose or CFU of a probiotic is variable. Three studies with 1010 CFU probiotic counts, six studies with about 109 CFU probiotic counts, and only one study with 107 CFU probiotic counts were included and evaluated. Thus, it seemingly that the dose of more than 107 CFU probiotic counts could show beneficial effects of probiotics supplementation on metabolic health in pregnant women. However, studies about specific doses of probiotics are limited, further studies about optimal dose or CFU of a probiotic supplementation in pregnant women are required. In addition, probiotic strains are also variable among the studies, and it is difficult to evaluate the effects of a specific probiotic species on metabolic health. Among the ten studies, Lactobacillus and Bifidobacterium were the mostly widely used strains. Thus, we speculated that Lactobacillus and Bifidobacterium may be beneficial probiotic strains for metabolic health in pregnant women. Currently, studies about the effects of specific probiotic strains are also limited. There is no consensus on the specific dose of probiotics and the ideal probiotic strains for the clinical intervention. Thus, further RCT studies that fully investigate and compare the efficacy among variable CFU doses and different probiotic strains are warranted, that are critically important to determine the optimal dose and ideal probiotic strains supplementation during pregnancy.
There are several limitations that should be considered: (1) the sample sizes were relatively small, ranging from 60 to 256 subjects. This might limit the power to estimate the effects of probiotics supplementation on pregnancy outcomes; (2) intervention durations of some studies are not clear, and several studies were with short duration, from diagnosis until delivery for 6–8 weeks; (3) the follow-up duration is short; (4) the stages of gestation are variable among healthy, obese pregnant women, and women with GDM, which can be a confounding factor for pooling the studies. Therefore, high-quality studies with longer intervention and larger sample size are needed.
To the best of our knowledge, this is the first systematic analysis to assess the effects of probiotics supplementation on metabolic health in the pregnant women, including healthy pregnant women, obese pregnant women and women with GDM. Despite the above limitations, this systematic analysis was more convincing than any previous single study. Indeed, our review has some notable strengths: (1) this review was strictly adhered to the PRISMA guidelines and PICO principle, and these methodologies can increase the robustness and validity of the results and conclusions; (2) only RCT studies were included in this review and all of them were objectively judged to be of high quality; (3) the parameters used to assess the clinical outcomes in all studies are strict and repeatable.
Next, the potential mechanisms underlying the beneficial effects of probiotics supplementation on pregnancy outcomes and metabolic health in pregnancy should be discussed [15]. Several potential points could explain the beneficial effects of probiotics during pregnancy. Pregnant women are susceptible to increased insulin resistance and glucose intolrance, and probiotics supplementation can improve glycemic control and insulin resistance. A systematic review with 17 RCTs showed that probiotic consumption significantly reduced FPG, fasting plasma insulin and HOMA-IR [38]. Oxidative stress has been shown to be present in hyperglycemia [39], and specific strains of lactic acid bacteria have been demonstrated to have antioxidant properties [40]. Moreover, probiotics can regulate the function of microbiota. Firstly, specific probiotics can balance the properties of aberrant indigenous microbiota [41]. Secondly, probiotics can ameliorate intestinal permeability [42]. It indicated that prebiotics improved gastric motility and gastric electrical activity in preterm newborns [43]. Thirdly, probiotics can regulate the secretion of proinflammatory cytokines [32]. It indicated that probiotics significantly decreased interleukin-6 (IL-6), tumor necrosis factor-a (TNF-a) and high sensitivity C reactive protein (hsCRP) levels. However, it is still unclear whether the impacts on the infants are due to the direct effects of probiotics through the milk or the placenta or adaptive responses to altered metabolism in mothers. Thus, further studies to elaborate the underlying mechanisms are urgently warranted.
Conclusions
In summary, the prevalence of GDM and maternal obesity is increasing rapidly worldwide. Intervention during pregnancy is proven to be challenging, with limited efficacy and low compliance. Our study demonstrated that probiotics supplementation during pregnancy has beneficial effects on glucose metabolic health among pregnant women, including women with GDM, and even healthy pregnant women. More importantly, the safety and easy implementation of probiotics supplementation has been widely accepted. A better understanding of the role of probiotics supplementation can provide critical implications for the early prevention and treatment of abnormal metabolic status among pregnant women, and thus ensure a healthier future for the mothers, infants, and even throughout young adulthood. However, high-quality, large-scale clinical trials are urgently warranted to assess the optimal dose and ideal bacterial composition of probiotics, and long-term outcome of probiotics among pregnant women.
References
- 1.
International Diabetes Federation. IDF Diabetes Atlas, 8th edn. 2017.
- 2. Pinhas-Hamiel O, Zeitler P. The global spread of type 2 diabetes mellitus in children and adolescents. The Journal of pediatrics. 2005;146(5):693–700. Epub 2005/05/05. pmid:15870677.
- 3. Rando OJ, Simmons RA. I'm eating for two: parental dietary effects on offspring metabolism. Cell. 2015;161(1):93–105. pmid:25815988; PubMed Central PMCID: PMCPMC4465102.
- 4. Patel N, Pasupathy D, Poston L. Determining the consequences of maternal obesity for offspring health. Exp Physiol. 2015;100(12):1421–8. pmid:26464224.
- 5. Stevens GA, Singh GM, Lu Y, Danaei G, Lin JK, Finucane MM, et al. National, regional, and global trends in adult overweight and obesity prevalences. Population health metrics. 2012;10(1):22. Epub 2012/11/22. pmid:23167948; PubMed Central PMCID: PMCPMC3543235.
- 6. Hillier TA, Pedula KL, Schmidt MM, Mullen JA, Charles MA, Pettitt DJ. Childhood obesity and metabolic imprinting: the ongoing effects of maternal hyperglycemia. Diabetes care. 2007;30(9):2287–92. Epub 2007/05/24. pmid:17519427.
- 7. Metzger BE, Lowe LP, Dyer AR, Trimble ER, Chaovarindr U, Coustan DR, et al. Hyperglycemia and adverse pregnancy outcomes. The New England journal of medicine. 2008;358(19):1991–2002. Epub 2008/05/09. pmid:18463375.
- 8. Catalano PM, McIntyre HD, Cruickshank JK, McCance DR, Dyer AR, Metzger BE, et al. The hyperglycemia and adverse pregnancy outcome study: associations of GDM and obesity with pregnancy outcomes. Diabetes care. 2012;35(4):780–6. Epub 2012/02/24. pmid:22357187; PubMed Central PMCID: PMCPmc3308300.
- 9. Kubo A, Ferrara A, Windham GC, Greenspan LC, Deardorff J, Hiatt RA, et al. Maternal hyperglycemia during pregnancy predicts adiposity of the offspring. Diabetes care. 2014;37(11):2996–3002. Epub 2014/08/26. pmid:25150158; PubMed Central PMCID: PMCPmc4207207.
- 10. Wallack L, Thornburg K. Developmental Origins, Epigenetics, and Equity: Moving Upstream. Maternal and child health journal. 2016;20(5):935–40. Epub 2016/04/01. pmid:27029539.
- 11. Baird J, Jacob C, Barker M, Fall CH, Hanson M, Harvey NC, et al. Developmental Origins of Health and Disease: A Lifecourse Approach to the Prevention of Non-Communicable Diseases. Healthcare (Basel, Switzerland). 2017;5(1). Epub 2017/03/12. pmid:28282852.
- 12. Barrett HL, Dekker Nitert M, Conwell LS, Callaway LK. Probiotics for preventing gestational diabetes. The Cochrane database of systematic reviews. 2014;(2):Cd009951. Epub 2014/02/28. pmid:24574258.
- 13. Sonnenburg JL, Backhed F. Diet-microbiota interactions as moderators of human metabolism. Nature. 2016;535(7610):56–64. Epub 2016/07/08. pmid:27383980.
- 14. Charbonneau MR, Blanton LV, DiGiulio DB, Relman DA, Lebrilla CB, Mills DA, et al. A microbial perspective of human developmental biology. Nature. 2016;535(7610):48–55. Epub 2016/07/08. pmid:27383979; PubMed Central PMCID: PMCPmc5358965.
- 15. Indrio F, Neu J. The intestinal microbiome of infants and the use of probiotics. Current opinion in pediatrics. 2011;23(2):145–50. Epub 2011/03/19. pmid:21415831; PubMed Central PMCID: PMCPmc3155417.
- 16. Indrio F, Di Mauro A, Riezzo G, Civardi E, Intini C, Corvaglia L, et al. Prophylactic use of a probiotic in the prevention of colic, regurgitation, and functional constipation: a randomized clinical trial. JAMA pediatrics. 2014;168(3):228–33. Epub 2014/01/16. pmid:24424513.
- 17. Liberati A, Altman DG, Tetzlaff J, Mulrow C, Gotzsche PC, Ioannidis JP, et al. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration. Journal of clinical epidemiology. 2009;62(10):e1–34. Epub 2009/07/28. pmid:19631507.
- 18. Moher D, Hopewell S, Schulz KF, Montori V, Gotzsche PC, Devereaux PJ, et al. CONSORT 2010 Explanation and Elaboration: Updated guidelines for reporting parallel group randomised trials. Journal of clinical epidemiology. 2010;63(8):e1–37. Epub 2010/03/30. pmid:20346624.
- 19. Moher D, Jadad AR, Tugwell P. Assessing the quality of randomized controlled trials. Current issues and future directions. International journal of technology assessment in health care. 1996;12(2):195–208. Epub 1996/01/01. pmid:8707495.
- 20. Higgins JP, Thompson SG, Deeks JJ, Altman DG. Measuring inconsistency in meta-analyses. BMJ (Clinical research ed). 2003;327(7414):557–60. Epub 2003/09/06. pmid:12958120; PubMed Central PMCID: PMCPmc192859.
- 21. Dickersin K, Berlin JA. Meta-analysis: state-of-the-science. Epidemiologic reviews. 1992;14:154–76. Epub 1992/01/01. pmid:1289110.
- 22. Chootrakool H, Shi JQ, Yue R. Meta-analysis and sensitivity analysis for multi-arm trials with selection bias. Statistics in medicine. 2011;30(11):1183–98. Epub 2011/05/04. pmid:21538449.
- 23. Laitinen K, Poussa T, Isolauri E. Probiotics and dietary counselling contribute to glucose regulation during and after pregnancy: a randomised controlled trial. The British journal of nutrition. 2009;101(11):1679–87. Epub 2008/11/20. pmid:19017418.
- 24. Luoto R, Laitinen K, Nermes M, Isolauri E. Impact of maternal probiotic-supplemented dietary counselling on pregnancy outcome and prenatal and postnatal growth: a double-blind, placebo-controlled study. The British journal of nutrition. 2010;103(12):1792–9. Epub 2010/02/05. pmid:20128938.
- 25. Ilmonen J, Isolauri E, Poussa T, Laitinen K. Impact of dietary counselling and probiotic intervention on maternal anthropometric measurements during and after pregnancy: a randomized placebo-controlled trial. Clinical nutrition (Edinburgh, Scotland). 2011;30(2):156–64. Epub 2010/10/26. pmid:20970896.
- 26. Asemi Z, Samimi M, Tabassi Z, Naghibi Rad M, Rahimi Foroushani A, Khorammian H, et al. Effect of daily consumption of probiotic yoghurt on insulin resistance in pregnant women: a randomized controlled trial. European journal of clinical nutrition. 2013;67(1):71–4. Epub 2012/11/29. pmid:23187955.
- 27. Jamilian M, Bahmani F, Vahedpoor Z, Salmani A, Tajabadi-Ebrahimi M, Jafari P, et al. Effects of Probiotic Supplementation on Metabolic Status in Pregnant Women: a Randomized, Double-blind, Placebo-Controlled Trial. Archives of Iranian medicine. 2016;19(10):687–2. Epub 2016/10/16. pmid:27743432.
- 28. Lindsay KL, Kennelly M, Culliton M, Smith T, Maguire OC, Shanahan F, et al. Probiotics in obese pregnancy do not reduce maternal fasting glucose: a double-blind, placebo-controlled, randomized trial (Probiotics in Pregnancy Study). The American journal of clinical nutrition. 2014;99(6):1432–9. Epub 2014/03/22. pmid:24646819.
- 29. Dolatkhah N, Hajifaraji M, Abbasalizadeh F, Aghamohammadzadeh N, Mehrabi Y, Abbasi MM. Is there a value for probiotic supplements in gestational diabetes mellitus? A randomized clinical trial. Journal of health, population, and nutrition. 2015;33:25. Epub 2016/01/31. pmid:26825666; PubMed Central PMCID: PMCPmc5026018.
- 30. Lindsay KL, Brennan L, Kennelly MA, Maguire OC, Smith T, Curran S, et al. Impact of probiotics in women with gestational diabetes mellitus on metabolic health: a randomized controlled trial. American journal of obstetrics and gynecology. 2015;212(4):496.e1–11. Epub 2015/02/18. pmid:25687568.
- 31. Karamali M, Dadkhah F, Sadrkhanlou M, Jamilian M, Ahmadi S, Tajabadi-Ebrahimi M, et al. Effects of probiotic supplementation on glycaemic control and lipid profiles in gestational diabetes: A randomized, double-blind, placebo-controlled trial. Diabetes & metabolism. 2016;42(4):234–41. Epub 2016/05/23. pmid:27209439.
- 32. Jafarnejad S, Saremi S, Jafarnejad F, Arab A. Effects of a Multispecies Probiotic Mixture on Glycemic Control and Inflammatory Status in Women with Gestational Diabetes: A Randomized Controlled Clinical Trial. Journal of nutrition and metabolism. 2016;2016:5190846. Epub 2016/07/19. pmid:27429803; PubMed Central PMCID: PMCPmc4939193.
- 33. Ohno MS, Sparks TN, Cheng YW, Caughey AB. Treating mild gestational diabetes mellitus: a cost-effectiveness analysis. American journal of obstetrics and gynecology. 2011;205(3):282.e1–7. Epub 2011/11/11. pmid:22071065; PubMed Central PMCID: PMCPmc3443977.
- 34. Chuang CH, Weisman CS, Hillemeier MM, Schwarz EB, Camacho FT, Dyer AM. Pregnancy intention and health behaviors: results from the Central Pennsylvania Women's Health Study cohort. Maternal and child health journal. 2010;14(4):501–10. Epub 2009/02/14. pmid:19214724; PubMed Central PMCID: PMCPmc2896424.
- 35. Poston L, Bell R, Croker H, Flynn AC, Godfrey KM, Goff L, et al. Effect of a behavioural intervention in obese pregnant women (the UPBEAT study): a multicentre, randomised controlled trial. The lancet Diabetes & endocrinology. 2015;3(10):767–77. Epub 2015/07/15. pmid:26165396.
- 36. Han S, Middleton P, Shepherd E, Van Ryswyk E, Crowther CA. Different types of dietary advice for women with gestational diabetes mellitus. The Cochrane database of systematic reviews. 2017;2:Cd009275. Epub 2017/02/27. pmid:28236296.
- 37. Taylor BL, Woodfall GE, Sheedy KE, O'Riley ML, Rainbow KA, Bramwell EL, et al. Effect of Probiotics on Metabolic Outcomes in Pregnant Women with Gestational Diabetes: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Nutrients. 2017;9(5). Epub 2017/05/06. pmid:28475161; PubMed Central PMCID: PMCPMC5452191.
- 38. Ruan Y, Sun J, He J, Chen F, Chen R, Chen H. Effect of Probiotics on Glycemic Control: A Systematic Review and Meta-Analysis of Randomized, Controlled Trials. PloS one. 2015;10(7):e0132121. Epub 2015/07/15. pmid:26161741; PubMed Central PMCID: PMCPmc4498615.
- 39. Ferreira L, Teixeira-de-Lemos E, Pinto F, Parada B, Mega C, Vala H, et al. Effects of sitagliptin treatment on dysmetabolism, inflammation, and oxidative stress in an animal model of type 2 diabetes (ZDF rat). Mediators of inflammation. 2010;2010:592760. Epub 2010/07/24. pmid:20652060; PubMed Central PMCID: PMCPmc2905949.
- 40. Amaretti A, di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A. Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Applied microbiology and biotechnology. 2013;97(2):809–17. Epub 2012/07/14. pmid:22790540.
- 41. Dahiya DK, Renuka , Puniya M, Shandilya UK, Dhewa T, Kumar N, et al. Gut Microbiota Modulation and Its Relationship with Obesity Using Prebiotic Fibers and Probiotics: A Review. Frontiers in microbiology. 2017;8:563. Epub 2017/04/20. pmid:28421057; PubMed Central PMCID: PMCPmc5378938.
- 42. Rokana N, Singh R, Mallappa RH, Batish VK, Grover S. Modulation of intestinal barrier function to ameliorate Salmonella infection in mice by oral administration of fermented milks produced with Lactobacillus plantarum MTCC 5690—a probiotic strain of Indian gut origin. Journal of medical microbiology. 2016;65(12):1482–93. Epub 2016/12/03. pmid:27902414.
- 43. Indrio F, Riezzo G, Raimondi F, Francavilla R, Montagna O, Valenzano ML, et al. Prebiotics improve gastric motility and gastric electrical activity in preterm newborns. Journal of pediatric gastroenterology and nutrition. 2009;49(2):258–61. Epub 2009/06/30. pmid:19561548.