Skip to main content
SpringerLink
Log in

Rapid Genetic Diagnosis With the Transcription—Reverse Transcription Concerted Reaction System for Cancer Micrometastasis

  • Original Articles
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Background: Detection of cancer micrometastases is required for improvement of cancer therapy. The aim of this study was to establish a rapid and practical genetic assay to detect micrometastasis in gastric cancer and to assess its clinical significance with respect to prognosis.

Methods: A novel RNA amplification system with transcription–reverse transcription concerted reaction (TRC) was introduced for quantitative detection of cancer-specific carcinoembryonic antigen messenger RNA. The sensitivity and quantitative aspects of the assay were assessed with the full-length carcinoembryonic antigen messenger RNA, a gastric cancer cell line (MKN-45), and metastatic lymph nodes obtained from patients with gastric cancer. Peritoneal lavage fluid specimens that were collected from gastric cancer surgery were subjected to the assay, and the clinical significance of the results was examined for prediction of recurrence and survival.

Results: The quantification, sensitivity, and reproducibility of the assay with the TRC reaction were equal to those of quantitative reverse transcriptase-polymerase chain reaction with LightCycler. The most important advantages of the assay were its simplicity and rapidity. Molecular diagnosis of peritoneal lavage fluid by the TRC reaction significantly correlated with depth of invasion, peritoneal metastasis, clinical stage, overall survival, and peritoneal recurrence-free survival.

Conclusions: Molecular diagnosis of peritoneal lavage fluid with the TRC reaction could be a useful prognostic indicator for peritoneal recurrence and survival. Because the TRC reaction is more rapid and simpler than reverse transcriptase-polymerase chain reaction as a format for detecting RNA sequences, it may enhance the genetic diagnosis of cancer micrometastasis and may improve cancer therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Raj GV, Moreno JG, Gomella LG. Utilization of polymerase chain reaction technology in the detection of solid tumors. Cancer 1998; 82: 1419–42.

    Google Scholar 

  2. Datta YH, Adams PT, Drobyski WR, Ethier SP, Terry VH, Roth MS. Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction. J Clin Oncol 1994; 12: 475–82.

    Google Scholar 

  3. Mori M, Mimori K, Inoue H, Barnard GF, Tsuji K, Nanbara S. Detection of cancer micrometastases in lymph nodes by reverse transcriptase-polymerase chain reaction. Cancer Res 1995; 55: 3417–20.

    CAS  PubMed  Google Scholar 

  4. Noguchi S, Aihara T, Motomura K, Inaji H, Imaoka S, Koyama H. Detection of breast cancer micrometastasis in axillary lymph nodes by means of reverse-transcriptase polymerase chain reaction. Am J Pathol 1996; 148: 649–56.

    Google Scholar 

  5. Hayashi N, Ito I, Yanagisawa A, et al. Genetic diagnosis of lymph-node metastasis in colorectal cancer. Lancet 1995; 345: 1257–9.

    CAS  PubMed  Google Scholar 

  6. Liefers GJ, Jansen AMC, Velde CJH, et al. Micrometastasis and survival in stage II colorectal cancer. N Engl J Med 1998; 339: 223–8.

    Article  CAS  PubMed  Google Scholar 

  7. Miyake Y, Fujiwara Y, Ohue M, et al. Quantification of micrometastasis in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction. Int J Oncol 2000; 16: 289–93.

    Google Scholar 

  8. Ishiguro T, Saitoh J, Horie R, et al. Intercalation activating fluorescence DNA probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel. Anal Biochem 2003; 314: 77–86.

    Google Scholar 

  9. Baba H, Korenaga D, Okamura T, Saito A, Sugimachi K. Prognostic factors in gastric cancer with serosal invasion. Arch Surg 1989; 124: 1061–4.

    Google Scholar 

  10. Hiratsuka M, Iwanaga T, Furukawa H, et al. Important prognostic factors in surgically treated gastric cancer patients. Gan To Kagaku Ryoho 1995; 22: 703–8.

    Google Scholar 

  11. Yoo CH, Noh SH, Shin DW, Choi SH, Min JS. Recurrence following curative resection for gastric carcinoma. Br J Surg 2000; 87: 236–42.

    Google Scholar 

  12. Koga S, Kaibara N, Iitsuka Y, Kudo H, Kimura A, Hiraoka H. Prognostic significance of intraperitoneal free cancer cells in gastric patients. J Cancer Res Clin Oncol 1984; 108: 236–8.

    CAS  PubMed  Google Scholar 

  13. Abe S, Yoshimura H, Tabara H, et al. Curative resection of gastric cancer: limitation of peritoneal lavage cytology in predicting the outcome. J Surg Oncol 1995; 59: 226–9.

    Google Scholar 

  14. Ribeiro U Jr, Gama-Rodrigues JJ, Safatle-Ribeiro AV, et al. Prognostic significance of intraperitoneal free cancer cells obtained by laparoscopic peritoneal lavage in patients with gastric cancer. J Gastrointest Surg 1998; 2: 244–9.

    Google Scholar 

  15. Bando E, Yonemura Y, Takeshita Y, et al. Intraoperative lavage for cytological examination in 1,297 patients with gastric carcinoma. Am J Surg 1999; 178: 256–62.

    Article  CAS  PubMed  Google Scholar 

  16. Nakanishi H, Kodera Y, Yamamura Y, et al. Rapid quantitative detection of carcinoembryonic antigen-expressing free tumor cells in the peritoneal cavity of gastric-cancer patients with real-time RT-PCR on the LightCycler. Int J Cancer 2000; 89: 411–7.

    Google Scholar 

  17. Nakanishi H, Kodera Y, Torii A. Detection of carcinoembryonic antigen-expressing free tumor cells in peritoneal washes from patients with gastric carcinoma by polymerase chain reaction. Jpn J Cancer Res 1997; 88: 687–92.

    Google Scholar 

  18. Kodera Y, Nakanishi H, Yamamura Y, et al. Prognostic value and clinical implications of disseminated cancer cells in the peritoneal cavity detected by reverse transcriptase-polymerase chain reaction and cytology. Int J Cancer 1998; 79: 429–33.

    Google Scholar 

  19. Kodera Y, Nakanishi H, Ito S, et al. Quantitative detection of disseminated free cancer cells in peritoneal washes with real-time reverse transcriptase-polymerase chain reaction: a sensitive predictor of outcome for patients with gastric carcinoma. Ann Surg 2002; 235: 499–506.

    Google Scholar 

  20. Ueno H, Yoshida K, Hirai T, Kono F, Kambe M, Toge T. Quantitative detection of carcinoembryonic antigen messenger RNA in the peritoneal cavity of gastric cancer patients by real-time quantitative reverse transcription polymerase chain reaction. Anticancer Res 2003; 23: 1701–8.

    Google Scholar 

  21. Sobin LH. TNM, sixth edition: new developments in general concepts and rules. Semin Surg Oncol 2003; 21: 19–22.

    Article  PubMed  Google Scholar 

  22. Yoshioka S, Fujiwara Y, Sugita Y, et al. Real-time rapid reverse transcriptase-polymerase chain reaction for intraoperative diagnosis of lymph node micrometastasis: clinical application for cervical lymph node dissection in esophageal cancers. Surgery 2002; 132: 34–40.

    Google Scholar 

  23. Mori T, Fujiwara Y, Sugita Y, et al. Application of molecular diagnosis for detection of peritoneal micrometastasis and evaluation of preoperative chemotherapy in advanced gastric carcinoma. Ann Surg Oncol 2004; 11: 14–20.

    Google Scholar 

  24. Morton DL, Wen DR, Wong JH, et al. Technical details of intraoperative lymphatic mapping for early stage melanoma. Arch Surg 1992; 127: 392–9.

    CAS  PubMed  Google Scholar 

  25. Giuliano AE, Kirgan DM, Guenther JM, Morton DL. Lymphatic mapping and sentinel lymphadenectomy for breast cancer. Ann Surg 1994; 220: 391–401.

    PubMed  Google Scholar 

  26. Kitagawa Y, Fujii H, Mukai M, et al. The role of the sentinel lymph node in gastrointestinal cancer. Surg Clin North Am 2000; 80: 1799–809.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan

    Takaaki Ishii MD, Yoshiyuki Fujiwara MD, Hirokazu Taniguchi MD, Shuji Takiguchi MD, Takushi Yasuda MD, Masahiko Yano MD & Morito Monden MD

  2. Scientific Instruments Division, Tosoh Corporation, Kanagawa, Japan

    Satoru Ohnaka & Toshinori Hayashi

  3. Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, 2-2 E2, Yamadaoka, Suita-City, Osaka, 565-0871, Japan

    Yoshiyuki Fujiwara MD

Authors
  1. Takaaki Ishii MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshiyuki Fujiwara MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Satoru Ohnaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Toshinori Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hirokazu Taniguchi MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shuji Takiguchi MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Takushi Yasuda MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Masahiko Yano MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Morito Monden MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yoshiyuki Fujiwara MD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ishii, T., Fujiwara, Y., Ohnaka, S. et al. Rapid Genetic Diagnosis With the Transcription—Reverse Transcription Concerted Reaction System for Cancer Micrometastasis. Ann Surg Oncol 11, 778–785 (2004). https://doi.org/10.1245/ASO.2004.12.043

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1245/ASO.2004.12.043

Key Words:

Search

Navigation