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Free radical reaction products and antioxidant capacity in beating heart coronary artery surgery compared to conventional bypass

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Abstract

Oxygen-derived free radicals are important agents of tissue injury during ischemia and reperfusion. The aim of this study was to investigate changes in protein and lipid oxidation and antioxidant status in beating heart coronary artery surgery and conventional bypass and to compare oxidative stress parameters between the two bypass methods. Serum lipid hydroperoxide, nitric oxide, protein carbonyl, nitrotyrosine, vitamin E, and β-carotene levels and total antioxidant capacity were measured in blood of 30 patients undergoing beating heart coronary artery surgery (OPCAB, off-pump coronary artery bypass grafting) and 12 patients undergoing conventional bypass (CABG, on-pump coronary artery bypass grafting). In the OPCAB group, nitric oxide and nitrotyrosine levels decreased after reperfusion. Similarly, β-carotene level and total antioxidant capacity also decreased after anesthesia and reperfusion. In the CABG group, nitric oxide and nitrotyrosine levels decreased after ischemia and reperfusion. However, protein carbonyl levels elevated after ischemia and reperfusion. Vitamin E, β-carotene, and total antioxidant capacity decreased after ischemia and reperfusion. Significantly decreased nitration and impaired antioxidant status were seen after reperfusion in both groups. Moreover, elevated protein carbonyls were found in the CABG group. The off-pump procedure is associated with lower degree of oxidative stress than on-pump coronary surgery.

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Abbreviations

CABG:

on-pump coronary artery bypass grafting

LPx:

lipid hydroperoxide

NOx:

nitric oxide

NT:

nitrotyrosine

OPCAB:

off-pump coronary artery bypass grafting

ROS:

reactive oxygen species

TAC:

total antioxidant capacity

References

  1. Garlick, P. B., Davies, M. J., Hearse, D. J., and Slater, T. F. (1987) Circ. Res., 61, 757–760.

    PubMed  CAS  Google Scholar 

  2. Flaherty, J. T., and Weisfeldt, M. L. (1988) Free Radic. Biol. Med., 5, 409–419.

    Article  PubMed  CAS  Google Scholar 

  3. McCord, J. M. (1988) Free Radic. Biol. Med., 4, 9–14.

    Article  PubMed  CAS  Google Scholar 

  4. Kuzuya, T., Hoshida, S., Kim, Y., Nishida, M., Fuji, H., Kitabatake, A., Tada, M., and Kamada, T. (1990) Circ. Res., 66, 1160–1165.

    PubMed  CAS  Google Scholar 

  5. Ambrosio, G., Zweier, J. L., and Flaherty, J. T. (1991) J. Mol. Cell Cardiol., 23, 1359–1374.

    Article  PubMed  CAS  Google Scholar 

  6. Henry, T. D., Archer, S. L., Nelson, D., Weir, E. K., and From, A. H. L. (1993) Am. J. Physiol., 264, H1478–H1484.

    PubMed  CAS  Google Scholar 

  7. Tosaki, A., Bagchi, D., Pali, T., Cordis, G. A., and Das, D. K. (1993) Biochem. Pharmacol., 45, 961–969.

    Article  PubMed  CAS  Google Scholar 

  8. Xia, Y., Khatchikian, G., and Zweier, J. L. (1996) J. Biol. Chem., 271, 10096–10102.

    Article  PubMed  CAS  Google Scholar 

  9. Hawkins, C. L., and Davies, M. J. (2001) Biochim. Biophys. Acta, 899, 196–219.

    Google Scholar 

  10. Kaneda, H., Taguchi, J., Ogasawara, K., Aizawa, T., and Ohno, M. (2002) Atherosclerosis, 162, 221–225.

    Article  PubMed  CAS  Google Scholar 

  11. Hayashi, Y., Sawa, Y., Ohtake, S., Fukuyama, N., Nakazawa, H., and Matsuda, H. (2001) Ann. Thorac. Surg., 72, 571–576.

    Article  PubMed  CAS  Google Scholar 

  12. Hayashi, Y., Sawa, Y., Fukuyama, N., Miyamoto, Y., Takahashi, T., Nakazawa, H., and Matsuda, H. (2003) J. Thorac. Cardiovasc. Surg., 126, 1813–1821.

    Article  PubMed  Google Scholar 

  13. Gutteridge, J. M. C. (1995) Clin. Chem., 41, 1819–1828.

    PubMed  CAS  Google Scholar 

  14. Nagel, E., Meyer, Z. U., Vilsendorf, A., Bartels, M., and Pichlmayr, R. (1997) Int. J. Vitam. Nutr. Res., 67, 298–306.

    PubMed  CAS  Google Scholar 

  15. Muzakova, V., Kandar, R., Vojtisek, P., Skalicky, J., Vankova, R., Cegan, A., and Certinkova, Z. (2001) Physiol. Res., 50, 389–396.

    PubMed  CAS  Google Scholar 

  16. Mezzetti, A., Lapenna, D., Pierdomenico, S. D., Di Giammarco, G., Bosco, G., Di Ilio, C., Santarelli, P., Calafiore, A. M., and Cuccurullo, F. (1993) J. Card. Surg., 8, 167–171.

    Article  PubMed  CAS  Google Scholar 

  17. Coghlan, J. G., Flitter, W. D., Clutton, S. M., Panda, R., Daly, R., Wright, G., Ilsley, C. D., and Slater, T. F. (1994) J. Thorac. Cardiovasc. Surg., 107, 248–256.

    PubMed  CAS  Google Scholar 

  18. De Vecchi, E., Pala, M. G., Di Credico, G., Agape, V., Paolini, G., Bonini, P. A., Grossi, A., and Paroni, R. (1998) Heart, 79, 242–247.

    PubMed  Google Scholar 

  19. Nourooz-Zadeh, J., Tajaddini-Sarmadi, J., and Wolff, S. P. (1994) Anal. Biochem., 220, 403–409.

    Article  PubMed  CAS  Google Scholar 

  20. Carty, J. L., Bevan, R., Waller, H., Mistry, N., Cooke, M., Lunec, J., and Griffiths, H. R. (2000) Biochem. Biophys. Res. Commun., 273, 729–735.

    Article  PubMed  CAS  Google Scholar 

  21. Griffiths, H. R., Aldred, S., Dale, C., Nakano, E., Kitas, G. D., Grant, M. G., Nugent, D., Taiwo, F. A., Li, L., and Powers, H. J. (2006) Free Radic. Biol. Med., 40, 488–500.

    Article  PubMed  CAS  Google Scholar 

  22. Lee, B. L., Chua, S. C., Ong, H. Y., and Ong, C. N. (1992) J. Chromatogr., 581, 41–47.

    Article  PubMed  CAS  Google Scholar 

  23. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. (1999) Free Radic. Biol. Med., 26, 1231–1237.

    Article  PubMed  CAS  Google Scholar 

  24. Halliwell, B., and Gutteridge, J. (1999) Free Radicals in Biology and Medicine, Oxford University Press, New York.

    Google Scholar 

  25. Tortolani, A. J., Powell, S. R., Misik, V., Weglicki, W. B., Pogo, G. J., and Kramer, J. H. (1993) Free Radic. Biol. Med., 14, 421–426.

    Article  PubMed  CAS  Google Scholar 

  26. Cavarrochi, N. C., England, M. D., and O’Brain, J. F. (1986) J. Surg. Res., 40, 519–527.

    Article  Google Scholar 

  27. Coghlan, J. G., Flitter, W. D., Clutton, S. M., Ilsley, C. D., Rees, A., and Slater, T. F. (1993) J. Thorac. Cardiovasc. Surg., 106, 268–274.

    PubMed  CAS  Google Scholar 

  28. Davies, S. W., Duffy, J. P., Wickens, D. G., Underwood, S. M., Hill, A., Alladine, M. F., Feneck, R. O., Dormandy, T. L., and Walesby, R. K. (1993) J. Thorac. Cardiovasc. Surg., 105, 979–987.

    PubMed  CAS  Google Scholar 

  29. Eiselt, J., Racek, J., and Opatrny, K. J. (2001) Cas. Lek. Cesk., 140, 238.

    PubMed  CAS  Google Scholar 

  30. Matata, B. M., Sosnowski, A. W., and Galinanes, M. (2000) Ann. Thorac. Surg., 69, 785–791.

    Article  PubMed  CAS  Google Scholar 

  31. Starkopf, J., Tamme, K., Zilmer, M., Talvik, R., and Samarutel, J. (1997) Clin. Chim. Acta, 262, 77–88.

    Article  PubMed  CAS  Google Scholar 

  32. Hadjinikolaou, L., Alexiou, C., Cohen, A. S., Standbridge, R. D. L., McColl, A. J., and Richmond, W. (2003) Eur. J. CardioThoracic Surg., 23, 969–975.

    Article  Google Scholar 

  33. Carlucci, F., Tabucchi, A., Biagioli, B., Simeone, F., Scoletta, S., Rosi, F., and Marinello, E. (2002) Biomed. Pharmacother., 56, 483–491.

    Article  PubMed  CAS  Google Scholar 

  34. Gerritsen, W. B., Boven, W. J., Driessen, A. H., Haas, J. F., and Aarts, L. P. (2001) Eur. J. Cardio-Thorac., 20, 923–929.

    Article  CAS  Google Scholar 

  35. Stadtman, E. R., and Oliver, C. N. (1991) J. Biol. Chem., 266, 2005–2008.

    PubMed  CAS  Google Scholar 

  36. Berlett, B. S., and Stadtman, E. R. (1997) J. Biol. Chem., 272, 20313–20316.

    Article  PubMed  CAS  Google Scholar 

  37. Uchida, K. (2000) Free Radic. Biol. Med., 28, 1685–1696.

    Article  PubMed  CAS  Google Scholar 

  38. Stadtman, E. R., and Berlett, B. S. (1997) Chem. Res. Toxicol., 10, 485–494.

    Article  PubMed  CAS  Google Scholar 

  39. Serdar, Z., Aslan, K., Dirican, M., Sarandol, E., Yesilbursa, D., and Serdar, A. (2006) Clin. Biochem., 39, 794–803.

    Article  PubMed  CAS  Google Scholar 

  40. Fu, S., Davies, M. J., Stocker, R., and Dean, R. T. (1998) Biochem. J., 333, 519–525.

    PubMed  CAS  Google Scholar 

  41. Kinlay, S., Libby, P., and Ganz, P. (2001) Curr. Opin. Lipidol., 12, 383–389.

    Article  PubMed  CAS  Google Scholar 

  42. Moncada, S. (1999) J. R. Soc. Med., 92, 164–169.

    PubMed  CAS  Google Scholar 

  43. Ignarro, L. J., Napoli, C., and Loscalzo, J. (2002) Circ. Res., 90, 21–28.

    Article  PubMed  CAS  Google Scholar 

  44. Node, K., Kitakaze, M., Kosaka, H., Komamura, K., Minamino, T., Tada, M., Inoue, M., Hori, M., and Kamada, T. (1995) Biochem. Biophys. Res. Commun., 211, 370–374.

    Article  PubMed  CAS  Google Scholar 

  45. Node, K., Kitakaze, M., Sato, H., Koretsune, Y., Karita, M., Kosaka, H., and Hori, M. (1998) J. Am. Coll. Cardiol., 32, 63–68.

    Article  PubMed  CAS  Google Scholar 

  46. Ma, X. L., Weyrich, A. S., Lefer, D. J., and Lefer, A. M. (1993) Circ. Res., 72, 403–412.

    PubMed  CAS  Google Scholar 

  47. Gohra, H., Fujimura, Y., Hamano, K., Hiroshi, N., Tomoe, K., Nobuya, Z., Kensuke, E., Toshiko, U., Daikai, S., and Tsuyoshi, M. (1999) World J. Surg., 23, 1249–1253.

    Article  PubMed  CAS  Google Scholar 

  48. Fukuyama, N., Takebayashi, Y., Hida, M., Ishida, H., Ichimori, K., and Nakazawa, H. (1997) Free Radic. Biol. Med., 22, 771–774.

    Article  PubMed  CAS  Google Scholar 

  49. Miwa, K., Igawa, A., Nakagawa, K., Hirai, T., and Inoue, H. (1999) Cardiovasc. Res., 41, 291–298.

    Article  PubMed  CAS  Google Scholar 

  50. Persad, S., Takeda, S., Panagia, V., and Dhalla, N. S. (1997) J. Mol. Cell. Cardiol., 29, 545–558.

    Article  PubMed  CAS  Google Scholar 

  51. Price, J. E., and Fowkes, F. G. (1997) Eur. Heart J., 18, 719–727.

    PubMed  CAS  Google Scholar 

  52. Marchioli, R. (1999) Pharmacol. Res., 40, 227–238.

    Article  PubMed  CAS  Google Scholar 

  53. Tribble, D. L., AHA Science Advisory (1999) Circulation, 99, 591–595.

    PubMed  CAS  Google Scholar 

  54. Haramaki, N., Stewart, D. B., Aggarwal, S., Ikeda, H., Reznick, A. Z., and Packer, L. (1998) Free Radic. Biol. Med., 25, 329–339.

    Article  PubMed  CAS  Google Scholar 

  55. Barsacchi, R., Pelosi, G., Maffei, S., Baroni, M., Salvatore, L., Ursini, F., Verunelli, F., and Biagini, A. (1992) Int. J. Cardiol., 37, 339–343.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmacy, Gazi University, Ankara, Turkey

    A. Gonenc, A. Hacışevki, M. Torun & B. Simsek

  2. Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK

    H. R. Griffiths

  3. Department of Cardiac Surgery, Guven Hospital, Ankara, Turkey

    B. Bakkaloglu

Authors
  1. A. Gonenc
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  2. A. Hacışevki
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  3. H. R. Griffiths
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  4. M. Torun
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  5. B. Bakkaloglu
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  6. B. Simsek
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Correspondence to A. Gonenc.

Additional information

Published in Russian in Biokhimiya, 2011, Vol. 76, No. 6, pp. 829–839.

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Gonenc, A., Hacışevki, A., Griffiths, H.R. et al. Free radical reaction products and antioxidant capacity in beating heart coronary artery surgery compared to conventional bypass. Biochemistry Moscow 76, 677–685 (2011). https://doi.org/10.1134/S0006297911060083

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  • DOI: https://doi.org/10.1134/S0006297911060083

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